We narrowed to 612 results for: Sis
-
TypeBlog PostPublishedFeb. 13, 2024, 2:15 p.m....3D models of the human lymphatic system. PBMCs consist of various immune cells, and can be used as a whole...
-
K. phaffii: Rising to the Occasion
TypeBlog PostPublishedApril 4, 2023, 1 p.m....the strain tractable for peroxisomal studies on biogenesis, degradation, and proliferation. Genome manipulation... -
The Power Behind NGS Plasmid Validation: seqWell
TypeBlog PostPublishedApril 19, 2017, 3:25 p.m....technologies that permit large-scale creation and synthesis (“writing”) of longer pieces of synthetic DNA,... -
FPbase: A new community-editable fluorescent protein database
TypeBlog PostPublishedMay 16, 2018, 1 p.m....lineages, 2-photon spectra, more elaborate spectrum analysis tools, and a better way to store and represent... -
Photosensitizer Induced Cell Ablation with FAP-TAP MG-2I-dL5**
TypeBlog PostPublishedSept. 19, 2017, 1:20 p.m....., Vitriol, E., & Hahn, K. (2008). Chromophore-assisted laser inactivation in cell biology. Trends in ... -
Pushing the Limits of DNA Assembly
TypeBlog PostPublishedSept. 6, 2017, 1:01 p.m....HiFi can simplify multi-position site-directed mutagenesis by assembling multiple fragments containing the... -
Visualizing Translation at the Single Molecule Level
TypeBlog PostPublishedAug. 1, 2017, 1:15 p.m....References 1. Ingolia, Nicholas T., et al. “Genome-wide analysis in vivo of translation with nucleotide resolution... -
Fluorescent Protein Travel Awards - FLiPs and Fluorescent Protein Biosensors
TypeBlog PostPublishedJuly 27, 2017, 1:11 p.m....sample. The second sensor is a fusion protein consisting of sfGFP fused to a His-tagged ABC transporter... -
What Good Citizenship Can Do for Reproducibility in Science
TypeBlog PostPublishedNov. 7, 2019, 2:18 p.m....found, to providing reagents in an accessible and consistent way so other researchers can use them, to making... -
Celebrate National Book Month with Addgene
TypeBlog PostPublishedOct. 3, 2019, 12:47 p.m....plants, among others. I particularly enjoyed the emphasis that microbes are neither bad or good - they're... -
Revamp Your Lab Meetings With Creative Virtual Collaboration
TypeBlog PostPublishedFeb. 9, 2021, 2:15 p.m....industry”. To date, aside from doing science, he has assisted many researchers in gaining the confidence to ... -
Networking Follow-up: I Met a Person. Now What?
TypeBlog PostPublishedJan. 12, 2021, 2:15 p.m....touch with all of their connections on a regular basis. It is also not at all necessary. Once you’ve talked... -
25 Work From Home Tips for Scientists
TypeBlog PostPublishedMarch 19, 2020, 1:15 p.m....communities are looking for bench scientists to assist in labs that are testing for COVID-19. You can ... -
Optimizing Donor DNA for Enhanced CRISPR Genome Editing
TypeBlog PostPublishedMarch 24, 2016, 2:30 p.m....the break and 91bp on the PAM-proximal side, consistently supported higher frequencies (up to 60%!) of... -
Components of CRISPR/Cas9
TypeBlog PostPublishedFeb. 2, 2016, 5 p.m....historical notes CRISPR arrays in bacterial genomes consist of repeated elements separated by unique sequences... -
Addgene Welcomes John Doench to the Advisory Board
TypeBlog PostPublishedAug. 13, 2015, 2:30 p.m.... The Addgene Advisory Board consists of prominent members of the bioscience and tech transfer communities... -
Management for Scientists: Delegating is Key
TypeBlog PostPublishedFeb. 20, 2015, 8:30 p.m....new measure of success. It is imperative that you resist the temptation to “just do it myself” or micromanage... -
From Lab Bench to Leadership: Transitioning into Management as a PhD
TypeBlog PostPublishedJan. 21, 2025, 2:15 p.m....burdensome task of explaining the steps to a lab assistant. However, I recognized the need to shift my mindset... -
Viral Vectors 101: Gamma-Retroviral Packaging Systems
TypeBlog PostPublishedAug. 6, 2024, 1:15 p.m....polypurine tract; recognition site for proviral DNA synthesis. Increases transduction efficiency and transgene... -
15 Years of Addgene: The Top 15 Plasmids
TypeBlog PostPublishedJan. 8, 2019, 1:54 p.m....customizable sgRNA from the U6 promoter and puromycin resistance from the EF-1a promoter. It’s a 3rd generation...