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TypeBlog PostPublishedJune 17, 2021, 1:17 p.m.... light. Inspired by @UriAlonWeizmann, here my song about our paper recently published in @nchembio ...
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Plasmids 101: TOPO Cloning
TypeBlog PostPublishedOct. 27, 2016, 2:30 p.m.... Lianna Swanson... -
Hot Plasmids - June 2020 - Barcoded CRISPR Library, Sparse Cell Labeling, Calcineurin Reporter, and DNA Staining Dye Alternative
TypeBlog PostPublishedJune 2, 2020, 1:15 p.m....purification of tTALE-eGFP and tTALE-mCherry. In comparison to intercalating fluorescent dyes which can cause... -
Viral Genome Sequencing - A One Stop Shop for AAV Identity and Serotype Confirmation
TypeBlog PostPublishedApril 23, 2020, 1:15 p.m....strands that are packaged at the same frequency, we reasoned that, following DNA extraction, the [+] and [-... -
New and Upcoming Viral Vectors - December 2019
TypeBlog PostPublishedDec. 19, 2019, 2:40 p.m....Plasmid Serotype Name 124603 AAV9 pAAV-EF1a-DIO-ChrimsonR-mRuby2-KV2.1-WPRE-SV40 124650 AAV9 pAAV-CamKIIa-C1V1... -
Bright Monomeric Fluorescent Proteins: mNeonGreen, mTFP1, and mWasabi
TypeBlog PostPublishedApril 25, 2019, 3:01 p.m....Resources on Addgene.org Learn about the Michael Davidson fluorescent protein collection Check out Addgene... -
Tetbow: Bright Multicolor Labeling for Neuronal Tracing
TypeBlog PostPublishedJan. 24, 2019, 2:24 p.m....introduced at ~2 copies per cell per color, following a Poisson distribution. To overcome the limited expression... -
Identifying Sequence Elements with SnapGene's Feature Database
TypeBlog PostPublishedDec. 21, 2017, 2:06 p.m....mismatches or indels. Empirical tests indicated that a reasonable rule is to require at least 96% sequence identity... -
Scientific Peer-review: Providing Critical and Kind Feedback and Advocating for Open Science
TypeBlog PostPublishedAug. 8, 2019, 1:19 p.m....she will start her own research group at Boyce Tompson Institute. References Baldwin, Melinda. "Credibility... -
Comparing Cas9 to NgAgo: Can the Argonautes Best CRISPR?
TypeBlog PostPublishedJune 9, 2016, 2:30 p.m....shift won’t happen overnight, but there are a few reasons why you might choose NgAgo over CRISPR proteins... -
Plasmid Cloning by PCR
TypeBlog PostPublishedMarch 29, 2016, 2:30 p.m....to add overhangs to your insert of interest for Gibson assembly. The steps following primer design and... -
Rewiring Metabolic Circuitry with CRISPR RNA Scaffolds [Video]
TypeBlog PostPublishedApril 7, 2015, 4:21 p.m....functionality of the RNA scaffolds follows the rules of Watson-Crick base pairing, allowing for rational, straightforward... -
Hot Plasmids: FRET-Based Biosensors
TypeBlog PostPublishedMay 6, 2014, 1:07 p.m....contribution to the field is a series of fluorescence resonance energy transfer (FRET)-based calcium biosensors... -
Interview: Ed Boyden on Optogenetics, Neuroscience, and the Future of Neuroengineering
TypeBlog PostPublishedMarch 4, 2014, 4:49 p.m....maybe we could treat patients suffering from Parkinson's, chronic pain, deafness, or other conditions.... -
The MTA at Addgene
TypeBlog PostPublishedJuly 23, 2024, 2:17 p.m.... Rachel Leeson... -
Hot Plasmids - January 2023
TypeBlog PostPublishedJan. 31, 2023, 2:15 p.m....reproducibility of a (recombinant) monoclonal. Another reason is that some antibodies may just not perform as... -
With Our New Viral Service, We're Taking CRISPR Further!
TypeBlog PostPublishedDec. 6, 2016, 3:30 p.m....that total with the indicated function. One huge reason CRISPR has become such a popular genome editing... -
Virtual Science Conference Coming Up? Three Addgenies Share Their Experience
TypeBlog PostPublishedJune 30, 2020, 1:15 p.m....be more difficult in the virtual format than in-person. You might miss out on the short chats between ... -
Scientists Map the SARS-CoV-2-Human Interaction Network
TypeBlog PostPublishedApril 9, 2020, 1:15 p.m....takes time away from our research. For all these reasons, the QCRG is delighted to be partnering with Addgene... -
Hot Plasmids - December 2019 - CRISPR pooled library, LipoGlo, CRISPRa, and Voltage Imaging Tools
TypeBlog PostPublishedDec. 10, 2019, 1:58 p.m....the mouse CRISPR knockout library at Addgene. Henriksson et al., Cell, 2019. PubMed PMID: 30639098. LipoGlo...