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... gRNAs that target your list of selected genes. Start by choosing which “Reference Library” you’d like...

...reviewed in [Yan & Bruchez, 2015]). To get you started, two robust orthogonal labeling strategies are ...

...construct. DNA and gRNA are not to scale.   Let’s start with the simplest multiplexing situation: you only...

...Conclusion Finally, we can summarize the process from start to finish:   Figure 9: Cartoon summary of...

...plasmids here Check out the Yamanaka paper that started the field of iPSCs Find gene-specific plasmids ...

...in protein expression. (If you're just getting started, check out our Introduction to PCR.) But if you're...

...replace entire protein-coding regions (excluding start and stop codons) with kanamycin cassettes and were...

...mechanisms. Rare observations like these may be a starting point for future developments in the field of ...

... found at Addgene, so what are you waiting for? Start building up your own designer bacteria. St-Pierre...

...from full-length luciferase produced prior to the start of the screen is quickly removed. This system should...