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Showing: 481 - 500 of 919 results

  1. New Antibody Animation!

    Type
    Blog Post
    Published
    Feb. 22, 2024, 3:13 p.m.
    ...isotypes found in mammals.      Subscribe to Addgene's YouTube channel for more helpful animations, useful...

  2. Cloning Mammalian Cells with the Agarose Method

    Type
    Blog Post
    Published
    Sept. 7, 2017, 12:17 p.m.
    ...interest; more recently we have also been using Addgene CRISPR vectors to generate cell lines with knockouts...figure 1.   Find Additional Useful Protocols on Addgene's Protocol Pages Agarose cloning method procedure...som.umaryland.edu.   Additional Resources on the Addgene Blog Tips for a 1st Time CRISPR User (by a 1st...Plasmids 101: Mammalian Vectors Resources on Addgene.org Check Out Our Protocols Pages Browse Fluorescent...

  3. Quick Guide to Working with Drosophila Part 1: Getting Started with Flies

    Type
    Blog Post
    Published
    July 13, 2017, 2:30 p.m.
    ...own mutant flies. Find Drosophila Resources at Addgene The birds and the bees when it comes to fruit ...basic knowledge to start working with flies. Like Addgene, the Drosophila community is highly sharing-oriented... a “pound symbol”. Additional Resources on the Addgene Blog Methods for Site Specific Recombination in...Drosophila Learn about Cre-Lox Resources on Addgene.org Find Drosophila Plamids Drosophila CRISPR Resources...

  4. Deep Dive: Fixing and Permeabilizing for Immunofluorescence

    Type
    Blog Post
    Published
    Aug. 30, 2022, 1:15 p.m.
    ...Antibodies Resources on Addgene.org Find recombinant antibodies at Addgene Find plasmids encoding recombinant...the-process-of-fixation-and-the-nature-of-fixatives/  Additional resources on the Addgene blog: Antibodies 101: Introduction to Immunofluorescence...recombinant antibodies at Addgene  ...

  6. New Coomassie Protocol Video

    Type
    Blog Post
    Published
    Oct. 31, 2023, 1:15 p.m.
    ... complete collection of protocol videos on the Addgene YouTube page. ...

  8. New CRISPR Web Resources and #12DaysofCRISPR Recap

    Type
    Blog Post
    Published
    Dec. 12, 2017, 3:13 p.m.
    ...Since the start of the CRISPR revolution, Addgene has distributed over 100,000 CRISPR plasmids. But that...Editing the Epigenome (plus bonus podcast with Addgene Advisory Board member Alex Chavez) Day 11: Pooled...

  9. Who Gives a Tweet? 9 Facts About Scientists on Twitter

    Type
    Blog Post
    Published
    June 26, 2017, 1:31 p.m.
    ...scientistswhotweet. And don’t forget to follow us @Addgene.   References Ke, Q., Ahn, Y., & Sugimoto, C....scientist? Check out this post. Resources on the Addgene Blog 6 Tools & Tips: Online Social Networking ...

  10. Fluorescent Proteins 101: Fluorescent Biosensors

    Type
    Blog Post
    Published
    May 18, 2017, 2:30 p.m.
    ... OR enzyme activity. Small molecule biosensors Addgene has many sensors that measure the concentration...fluorescent molecule   Additional Resources on the Addgene Blog Check out the Rosella Autophagy Sensor Read...Addgenie Mary Gearing contributed to the content of this article. Biosensors (‘biological sensors’) are...CRISPRainbow for Genome Visualization Resources on Addgene.org Find Biosensor Plasmids Find Optogenetics Plasmids...

  11. SunTag and Fluorescent Imaging

    Type
    Blog Post
    Published
    March 28, 2017, 2:30 p.m.
    ...protein-by-protein basis. However, since many of Addgene’s SunTag plasmids have coveted “blue flames,” it...PMCID: PMC4252608. Additional Resources on the Addgene Blog Sign Up to Receive the Fluorescent Protein...about Small Molecule Fluorphores Resources on Addgene.org Find Fluorescent Protein Empy Backbones Visit...

  12. Using Ultrasound to Image Bacteria in vivo: Acoustic Reporter Genes

    Type
    Blog Post
    Published
    June 19, 2018, 1:38 p.m.
    ...research? arg1 and arg2 plasmids are available from Addgene! References 1 Bourdeau, R.W., Lee-Gosselin, A....PubMed PMID: 29300010. Additional Resources on the Addgene Blog Learn more about in vivo imaging tools The...fluorescent protein reporter gene Resources on Addgene.org Find plasmids for in vivo imaging with fluorescent...

  13. Plasmids 101: Positive and Negative Selection for Plasmid Cloning

    Type
    Blog Post
    Published
    Aug. 22, 2019, 12:43 p.m.
    ...counter-selection Resources on Addgene.org Find plasmids at Addgene Read our molecular biology reference...plasmid cloning. Additional resources on the Addgene blog Read our Plasmids 101 blog posts Learn more...

  14. X-CHIME: Context Dependent Germline Knockout in Immune Cells

    Type
    Blog Post
    Published
    Jan. 16, 2024, 6:13 p.m.
    ...new X-CHIME systems, developed and deposited at Addgene by Arlene Sharpe’s lab, allow for wide deployment....1038/s41467-019-09656-2 More resources on the Addgene blog CRISPR101: Validating Your Genome Edit How...Genome Editing CRISPR Cheat Sheat Resources from Addgene.org CRISPR Guide CRISPR: Protocol for Genome Deletions...

  15. IBEX Knowledge-Base: A Data Resource for Multiplex Tissue Imaging

    Type
    Blog Post
    Published
    June 8, 2023, 1:15 p.m.
    .../10.1073/pnas.2018488117 More resources on the Addgene blog Antibodies 101: Multiplex Immunofluorescence...Immunofluorescence Negative Can Be Positive: Open AAV Data with Addgene Antibodies 101: Choosing the Right Isotype...

  16. 9 Tips to Achieve Success in Academia

    Type
    Blog Post
    Published
    April 1, 2014, 5:33 p.m.
    ...Principal Investigators (PIs). We've interviewed Addgene depositors who are at various stages in their careers...journalism, entertainment, politics, K-12, etc.” Addgene's team includes many PhD scientists who have stepped...

  17. Tag Your Favorite Yeast Genes with Ease

    Type
    Blog Post
    Published
    Nov. 19, 2013, 2:37 p.m.
    ...product for isolation of successful transformants. Addgene distributes several ready-to-use, modular plasmids...few. Have you used these tools in your own lab? Addgene would love to hear from you, our community, about...

  18. Tips for arabidopsis transformation

    Type
    Blog Post
    Published
    Oct. 25, 2018, 1:23 p.m.
    ...Glazebrook, 2002). Find plant plasmid resources at Addgene  Choosing an expression system Choosing an appropriate...21 September 2018). Additional resources on the Addgene blog Engineering the plant genome using CRISPR...resource page Deposit arabidopsis plasmids with Addgene ...Using arabidopsis in the classroom Resources on Addgene.org Find plant plasmids Visit our plant resource...

  19. New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries

    Type
    Blog Post
    Published
    Oct. 4, 2018, 12:44 p.m.
    ...Resources on the Addgene Blog Read this blog post about CRISPR Kinome Libraries available at Addgene Learn about...Find pooled libraries available at Addgene Find CRISPRko (Brie), CRISPRi (Dolomiti), and CRISRPa (Caprano...genome-wide screening using CRISPR Resources on Addgene.org Find CRISPR plasmids and resources here Find...

  20. Neuronal labeling with Spaghetti Monster

    Type
    Blog Post
    Published
    Aug. 14, 2018, 12:42 p.m.
    ...and PNS Resources on Addgene.org Find the Spaghetti Monster probes at Addgene Browse all AAV vectors...University. Find the Spaghetti Monster probes at Addgene Uses of smFPs 1. Multi-channel connectomic tracers...PubMed PMID: 18057027. Additional Resources on the Addgene Blog Rabies and neuronal tracing Retrograde AAV...
Showing: 481 - 500 of 919 results