We narrowed to 529 results for: arl
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TypeBlog PostPublishedNov. 15, 2017, 3 p.m....generating a standard curve of known concentration. We regularly make and validate fresh plasmid standards. We...
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Transferable Skills Guide: Career Planning Resources
TypeBlog PostPublishedNov. 14, 2017, 2 p.m....to live in your lab, right? Wrong! While it is clearly a good idea to be diligent in your research, remember... -
"Hall of Fame" AAV Enhancers from the Allen Institute for Brain Science
TypeBlog PostPublishedSept. 9, 2025, 1:15 p.m....systemically (retro-orbitally, RO; or intracerebroventricularly, ICV) or locally (stereotaxically, STX... -
Tips and Tricks for Using Golden Gate Modular Cloning (MoClo)
TypeBlog PostPublishedJuly 8, 2025, 1:15 p.m....depends directly on the choices you make during the early stages of the process. Some questions to start with... -
Plasmids 101: Shuttle Vectors
TypeBlog PostPublishedMay 20, 2025, 1:15 p.m....machinery. Alternatively, some slow-growing cells, particularly mammalian cells like neurons, may not replicate... -
CRISPR-mediated Plant Base Editors
TypeBlog PostPublishedJan. 3, 2019, 1:35 p.m.... other proteins. You can detect base editing as early as 48-72 hours post-transformation/transfection ... -
Plasmids 101: Stringent Regulation of Replication
TypeBlog PostPublishedJune 20, 2024, 1:15 p.m....replication of the plasmid proceeds (and terminates) similarly to the bacterial chromosome. Since replication... -
Antibiotic Resistance: An Old Solution but a New Problem
TypeBlog PostPublishedNov. 10, 2016, 3:30 p.m....are partially resistant. If you stop a treatment early, you may be un-wittingly selecting for less susceptible... -
Plasmids 101: Control Plasmids
TypeBlog PostPublishedApril 9, 2015, 3:29 p.m....control, the internal control vector, was described earlier. Once you are sure your conditions are conducive... -
Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus
TypeBlog PostPublishedSept. 1, 2020, 1:15 p.m....several split vector approaches developed in the early 2000’s which allow researchers to deliver oversized... -
Transferable Skills Guide: Conflict Resolution
TypeBlog PostPublishedJan. 4, 2022, 2:15 p.m....identify and address these types of harmful conflict early, because time frequently does not heal these types... -
Replacing Paper: Tips for Choosing an Electronic lab Notebook
TypeBlog PostPublishedJuly 10, 2018, 1:08 p.m....Reproducibility Project: Cancer Biology have shown similarly disappointing rates of reproducibility. These ... -
Proximity Labeling: A Powerful Tool for Protein Complex Purification and Proteomic Mapping
TypeBlog PostPublishedDec. 2, 2019, 2 p.m....signal is significantly greater than miniTurbo at earlier time points but miniTurbo exhibits less labeling... -
Plasmids 101: Expressing shRNAs for RNA Interference
TypeBlog PostPublishedJune 17, 2025, 1:15 p.m.... G., Babina, M., Bertin, N., Burroughs, A. M., Carlisle, A. J., Daub, C. O., Detmar, M., Deviatiiarov,... -
An Integrin Antibody Toolkit from IPI
TypeBlog PostPublishedMarch 28, 2024, 1:15 p.m....intra- and extracellular environments. Found on nearly every cell in the human body, integrins are key... -
CRISPR 101: Homology Directed Repair
TypeBlog PostPublishedJan. 26, 2023, 2:15 p.m....vertically at the orange arrows). If resolved dissimilarly (e.g. one junction is resolved on the crossing... -
Deep Mutational Scanning with One Pot Saturation Mutagenesis
TypeBlog PostPublishedFeb. 22, 2017, 3:30 p.m....25167058. PubMed Central PMCID: PMC4412028. 5. Araya, Carlos L., and Douglas M. Fowler. "Deep mutational scanning... -
CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9
TypeBlog PostPublishedOct. 11, 2016, 2:30 p.m....peptides (Santana et al., 2020) and plants viruses (barley yellow striate mosaic virus (Gao et al., 2019) ... -
Multiple Plasmids at a Low Price: Kits from Addgene
TypeBlog PostPublishedSept. 14, 2016, 2:30 p.m....: 2388-2393. Huang, Shijie, Xuechen Zhu, and Charles E. Melançon III. "Detection and Quantification... -
Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?
TypeBlog PostPublishedMay 10, 2016, 2:30 p.m....systems. Both approaches may prove more successful early in the course of infection, when HIV-1 is limited...