We narrowed to 526 results for: arl

1. Viral Vectors 101: Preparing Pooled Libraries

   Type

   Blog Post

   Published

   July 13, 2023, 1:15 p.m.

   ...our more popular libraries, you might receive a similarly-sized aliquot from an amplification done by Addgene...

2. Plasmids 101: Stringent Regulation of Replication

   Type

   Blog Post

   Published

   June 20, 2024, 1:15 p.m.

   ...replication of the plasmid proceeds (and terminates) similarly to the bacterial chromosome. Since replication...

3. AAV Titers: Where Do They Come From And What Do They Mean?

   Type

   Blog Post

   Published

   Nov. 15, 2017, 3 p.m.

   ...generating a standard curve of known concentration. We regularly make and validate fresh plasmid standards. We...

4. Transferable Skills Guide: Career Planning Resources

   Type

   Blog Post

   Published

   Nov. 14, 2017, 2 p.m.

   ...to live in your lab, right? Wrong! While it is clearly a good idea to be diligent in your research, remember...

5. Antibiotic Resistance: An Old Solution but a New Problem

   Type

   Blog Post

   Published

   Nov. 10, 2016, 3:30 p.m.

   ...are partially resistant. If you stop a treatment early, you may be un-wittingly selecting for less susceptible...

6. Plasmids 101: Control Plasmids

   Type

   Blog Post

   Published

   April 9, 2015, 3:29 p.m.

   ...control, the internal control vector, was described earlier. Once you are sure your conditions are conducive...

7. Replacing Paper: Tips for Choosing an Electronic lab Notebook

   Type

   Blog Post

   Published

   July 10, 2018, 1:08 p.m.

   ...Reproducibility Project: Cancer Biology have shown similarly disappointing rates of reproducibility. These ...

8. Proximity Labeling: A Powerful Tool for Protein Complex Purification and Proteomic Mapping

   Type

   Blog Post

   Published

   Dec. 2, 2019, 2 p.m.

   ...signal is significantly greater than miniTurbo at earlier time points but miniTurbo exhibits less labeling...

9. Deep Mutational Scanning with One Pot Saturation Mutagenesis

   Type

   Blog Post

   Published

   Feb. 22, 2017, 3:30 p.m.

   ...25167058. PubMed Central PMCID: PMC4412028. 5. Araya, Carlos L., and Douglas M. Fowler. "Deep mutational scanning...

10. Transferable Skills Guide: Conflict Resolution

    Type

    Blog Post

    Published

    Jan. 4, 2022, 2:15 p.m.

    ...identify and address these types of harmful conflict early, because time frequently does not heal these types...

11. Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus

    Type

    Blog Post

    Published

    Sept. 1, 2020, 1:15 p.m.

    ...several split vector approaches developed in the early 2000’s which allow researchers to deliver oversized...

12. Plasmids 101: Expressing shRNAs for RNA Interference

    Type

    Blog Post

    Published

    June 17, 2025, 1:15 p.m.

    ... G., Babina, M., Bertin, N., Burroughs, A. M., Carlisle, A. J., Daub, C. O., Detmar, M., Deviatiiarov,...

13. An Integrin Antibody Toolkit from IPI

    Type

    Blog Post

    Published

    March 28, 2024, 1:15 p.m.

    ...intra- and extracellular environments. Found on nearly every cell in the human body, integrins are key...

14. CRISPR 101: Homology Directed Repair

    Type

    Blog Post

    Published

    Jan. 26, 2023, 2:15 p.m.

    ...vertically at the orange arrows). If resolved dissimilarly (e.g. one junction is resolved on the crossing...

15. CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

    Type

    Blog Post

    Published

    Oct. 11, 2016, 2:30 p.m.

    ...peptides (Santana et al., 2020) and plants viruses (barley yellow striate mosaic virus (Gao et al., 2019) ...

16. Multiple Plasmids at a Low Price: Kits from Addgene

    Type

    Blog Post

    Published

    Sept. 14, 2016, 2:30 p.m.

    ...: 2388-2393. Huang, Shijie, Xuechen Zhu, and Charles E. Melançon III. "Detection and Quantification...

17. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

    Type

    Blog Post

    Published

    May 10, 2016, 2:30 p.m.

    ...systems. Both approaches may prove more successful early in the course of infection, when HIV-1 is limited...

18. Technologies Enabled by NanoLuc® Luciferase

    Type

    Blog Post

    Published

    Feb. 8, 2018, 12:17 p.m.

    ...orange fluorescent protein, CyOFP1. CyOFP1 was molecularly evolved from mNeptune2 through 33 mutations and...

19. Viral Vectors 101: AAV Serotypes and Tissue Tropism

    Type

    Blog Post

    Published

    Jan. 16, 2025, 2:15 p.m.

    ...al., 2023; Wu, Asokan, et al., 2006). AAV6 is particularly well-suited for transduction of lung cells via...

20. Viral Vectors 101: Optogenetic Tools

    Type

    Blog Post

    Published

    June 27, 2023, 1:15 p.m.

    ...over when and where the receptor is activated.  Similarly, PTLs are special, covalently linked molecules...