We narrowed to 512 results for: ato

1. Hot Plasmids: Summer 2024

   Type

   Blog Post

   Published

   Aug. 27, 2024, 1:15 p.m.

   ...dCas9, zinc finger proteins, and transcription activator-like effectors (TALEs), with fifteen total variants...

2. Antibodies 101: Beyond Surface Labeling

   Type

   Blog Post

   Published

   June 27, 2024, 1:15 p.m.

   .... His projects include investigating negative regulators of T cells and cytokine signaling in tumor immunity...

3. Antibodies 101: Introduction to Gating in Flow Cytometry

   Type

   Blog Post

   Published

   April 4, 2024, 1:15 p.m.

   .... His projects include investigating negative regulators of T cells and cytokine signaling in tumor immunity...

4. Deep Mutational Scanning with One Pot Saturation Mutagenesis

   Type

   Blog Post

   Published

   Feb. 22, 2017, 3:30 p.m.

   ... combine pairs of mutations together to test combinatory effects. One-pot saturation mutagenesis The ...

5. Proximity Labeling: A Powerful Tool for Protein Complex Purification and Proteomic Mapping

   Type

   Blog Post

   Published

   Dec. 2, 2019, 2 p.m.

   ...Spatial Proximity." Journal of Investigative Dermatology 137.12 (2017): e197-e203. PubMed PMID: 29169465...

6. Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection

   Type

   Blog Post

   Published

   April 9, 2019, 1:13 p.m.

   ...et al. "Expanded dynamic range of fluorescent indicators for Ca2+ by circularly permuted yellow fluorescent...

7. Antibodies 101: Single Chain Fragment Variables (scFvs)

   Type

   Blog Post

   Published

   June 3, 2021, 1:15 p.m.

   ..., and enzymes for cancer, autoimmune, and/or inflammatory therapeutic applications. Antibody fragments...

8. Filming Science Videos in the Age of Social Distancing

   Type

   Blog Post

   Published

   May 20, 2021, 1:15 p.m.

   ... their mask. But while the camera is here, the operator is in a different room.   Design: The previous...

9. Fluorescent Proteins 101: When GFP lets you down

   Type

   Blog Post

   Published

   Aug. 23, 2018, 12:05 p.m.

   ...25831507 PubMed Central PMCID: PMC4394297. 16. Iwano, Satoshi, et al. "Single-cell bioluminescence imaging of...

10. Replacing Paper: Tips for Choosing an Electronic lab Notebook

    Type

    Blog Post

    Published

    July 10, 2018, 1:08 p.m.

    ...lab (user, date, time, action) are required for regulatory purposes in many labs. Besides this being of ...

11. Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus

    Type

    Blog Post

    Published

    Sept. 1, 2020, 1:15 p.m.

    ...10% expression in primary human T cells and hematopoietic stem and progenitor cells (HSPC)s to ~40% expression...

12. Site Directed Mutagenesis by PCR

    Type

    Blog Post

    Published

    Aug. 2, 2016, 2:30 p.m.

    ...introduce or remove restriction sites), in mapping of regulatory elements (to mutate promoters/enhancers in reporter...

13. Evolution of Lab Techniques

    Type

    Blog Post

    Published

    June 21, 2016, 2:30 p.m.

    ...DNA technologies that are now commonplace in laboratories. As scientific technologies rapidly progress...

14. Plasmids 101: Gibson Assembly and Other Long-Homology Based Cloning Methods

    Type

    Blog Post

    Published

    March 1, 2016, 3:30 p.m.

    ...loop (as might be anticipated to occur within a terminator sequence), since this would directly compete ...

15. Viral Vectors 101: AAV Serotypes and Tissue Tropism

    Type

    Blog Post

    Published

    Jan. 16, 2025, 3:42 p.m.

    ... receptor 1 (FGFR1)3 αVβ5 and α5β1 integrins3 Hepatocyte growth factor receptor (HGFR)3 Laminin receptor...

16. Viral Vectors 101: AAV Variables That Matter

    Type

    Blog Post

    Published

    June 1, 2023, 1:15 p.m.

    ...Response against the Tetracycline-Dependent Transactivator Correlates with Long-Term Doxycycline-Regulated...

17. Scientific Reproducibility - Focusing on Solutions at the Minisymposium on Reproducibility

    Type

    Blog Post

    Published

    May 18, 2018, 7:32 p.m.

    ... tries 5 separate experiments at once to get confirmatory data for a hypothesis and presents only the ...

18. Technique: Probe Phage Genomes for Host Binding Proteins

    Type

    Blog Post

    Published

    Sept. 29, 2016, 2:30 p.m.

    ...a hose to a nitrogen gas tank with a pressure regulator. Other methods for physical shearing exist as ...

19. Building Global Connections with the International Mentorship Program USA-EUROPE

    Type

    Blog Post

    Published

    Nov. 6, 2015, 3:30 p.m.

    ..., setting up bases and contacts with Spanish laboratories to start working on projects together. List ...

20. Lambda Red: A Homologous Recombination-based Technique for Genetic Engineering

    Type

    Blog Post

    Published

    Dec. 15, 2016, 3:57 p.m.

    ...currently a research scientist in the Department of Laboratory Medicine at the University of Washington. She...