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..., it’s essential to understand them in order to design the most specific assays possible. In this blog...-target effects should impact your experimental design differently. Yes, it is always better to reduce...introducing a specified edit). However, if your design does have wiggle room, you can minimize off targets...

...facilitate the selection of cells containing your desired edit. The selection is marker is then seamlessly...sites is cytotoxic for proliferating cells and provides a simple tool for conditional cell ablation.,”...Zinc-finger nuclease-induced gene repair with oligodeoxynucleotides: wanted and unwanted target locus modifications...

...experiments to minimize it. Overview of DIO/FLEx vector design Recombinase-dependent vectors utilize pairs of ...between them. Early recombinase-dependent vector designs used a lox-STOP-lox (LSL) or frt-STOP-frt (FSF)...you intend to use performs in your system. This includes a thorough testing of both the serotype and promoter...

...good starting point for most researchers as it provides a list of putative off-target sites that they ... approach misses larger mismatches (e.g. six nucleotides) that may still lead to off-target double-stranded...contains both the rare off target event and the desired edit is low. Therefore, unbiased sequencing may...

...tetroxide). These conditions destroy most PA-FPs, but, in 2015, the Looger Lab designed 2 variants of EosFP that...to green). For example, mEosFP (named after the goddess of dawn in Greek mythology) switches from green...

...in the development of CBEs and ABEs. This post includes a lot of acronyms, so we’ve included a glossary... in different cell types, available at www.crisprbehive.design. CGBEs fused to pioneer factors to improve...strand that does not base pair with the guide RNA. Despite being called the "non-target strand" in CRISPR ...

...second edition includes new and updated content in a clean and easy-to-navigate design. You can find it...

...quest, which led us to Seqera Labs, a company that provides open source workflow orchestration software for...on how Addgene has used Nextflow to improve and redesign our pipeline. In it, they break down the unique...

...#12DaysOfCRIPSR hashtag to share new CRISPR content designed to help you with your CRISPR experiments and to...CRISPR, Addgene gave to me: tips and tricks for designing gRNAs for CRISPR editing. On the fourth day of...

...distribution. Lab tip #3: Barcode everything Use barcodes and scanners to keep track of reagents in your...materials quickly and easily. Learn more about using barcodes. Check out our “Lab Tip” filter on the blog...

...anniversary! We've done so much in the past two (2!) decades that it's hard to summarize, but we wanted to mark...experience we're sharing through blogs, eBooks, videos, guides, protocols, and of course, the friendly folks at...

...and find our guides, plasmid kits and other resources for microbial research. Kits, guides, plasmids, ...

...article describing a sucrose supplementation strategy to improve yield. In addition, as described in this...

...CRISPR 101 eBook. This new edition of the eBook includes the details on advances in CRISPR including: ...bacteria that allow the bacteria to target and destroy viruses that infect them. Commonly referred to ...

...10,000 plasmid deposits per year in recent years. Despite COVID-19 and lab closures, we’re expecting to reach...globally. *All data presented in this blog post includes data until November 9, 2020. Between then and ...

...below the ordering table. Click on it, select your desired serotype and volume, and submit your request.  ...simply request your plasmid of interest in your desired serotype, place your order when ready, and we’ll...

...of lab materials to create their decorations! Despite all the craziness of 2020, for our Zoom #HolidayParty...microbial pigment. Check out the hashtag to see the description of all the ornaments. #DeckTheLab @Addgene pic.twitter.com...

...and point out one or more additional posts that deserve a peek in case you missed them. July 2016 was a...Fessenden at Brigham and Women's Hospital. This post describes how to target and use FRET flurophores for a variety...

... the United States while caring for a patient, despite wearing protective gear, and now comes news of ...Virginia School of Medicine have also recently described how the glycoprotein transforms itself to escape...

...First described in the 1980s, protein tags are now one of the most useful items in a scientist’s toolbox...IT10 expressed well in cells and did not form undesirable aggregates. Using budding yeast as a model system...