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Showing: 481 - 500 of 756 results

  1. Savvy Advocates Needed to Navigate a Scientific Enterprise in Flux

    Type
    Blog Post
    Published
    Sept. 13, 2016, 2:30 p.m.
    ...symposium includes a panel discussion and keynote by former congressman and CEO of the American Association...bootcamp” for a focused group of participants. More information can be found at http://futureofresearch.org/advocating-for-science-boston...symposium will culminate with a keynote address by former congressman and CEO of the American Association...

  2. Production of Virus in Insect Versus Mammalian Cells

    Type
    Blog Post
    Published
    Nov. 5, 2024, 8:32 p.m.
    ...understanding the differences can help you make an informed decision. Mammalian cells Mammalian cells are ...Ararat, M., & Graham, F. L. (2002). Preferential transformation of human neuronal cells by human adenoviruses... S., & Ng, S. K. (2021). HEK293 cell line as a platform to produce recombinant proteins and viral vectors...

  3. PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

    Type
    Blog Post
    Published
    June 25, 2024, 1:15 p.m.
    ...thousands of designs in parallel. To do this, we performed high-throughput ‘self-targeting’ screens with ...PRIDICT2.0 score. For the best prime editing performance, we suggest that users select some of the best...learning–based model with high generalization performance. Science Advances, 5(11), eaax9249. https://doi.org...

  4. Academic vs. Industry Postdocs

    Type
    Blog Post
    Published
    Nov. 13, 2018, 1:22 p.m.
    ...project will not involve proprietary compounds or information. Where publication is expected, the process is...ensure that it does not divulge any propriety information. Working as a team: Industry is all about teamwork...at once. Many companies will hire a postdoc to perform a specific project, while others will hire a group...

  5. Plasmids 101: 5 factors to help you choose the right cloning method

    Type
    Blog Post
    Published
    Aug. 21, 2018, 12:31 p.m.
    ...consider Gibson cloning. In this technique, you first perform a round of PCR to generate DNA fragments with 15...your insert, DpnI digest away the templates, and transform the PCR products into E. coli. Ligases within ...available cloning kits that one could choose from to perform these experiments. In most cases, there will be...

  6. Experimenting with New Careers while in Grad School

    Type
    Blog Post
    Published
    Feb. 25, 2016, 3:30 p.m.
    ...submitted was hilarious, full of interesting information, and received a lot of attention on social media...Museum and others when I was trying to find local informal science educators, and ever since, the State Museum...the network I built during graduate school is my informal advisory board. Although my goals may not have...

  7. Genome-wide Screening Using CRISPR

    Type
    Blog Post
    Published
    Aug. 18, 2015, 2:30 p.m.
    ...discuss how CRISPR libraries are being used to perform genome-wide screens and highlight some of the reagents...What are the steps involved in a CRISPR screen? Performing a forward genetic screen using CRISPR libraries...your phenotype of interest. For more detailed information on CRISPR screens check out Addgene’s 2016 publication...

  8. CRISPR 101: RNA Editing with Cas13

    Type
    Blog Post
    Published
    July 31, 2020, 12:30 p.m.
    ...resulted in 15% C-to-U editing activity. They then performed sixteen rounds of directed evolution across the... cytidine deaminase activity. While RESCUE can perform C-to-U edits, the authors note that it retains ...whole-transcriptome RNA-sequencing, the authors performed rational mutagenesis and identified that a S375A...

  9. Plasmids 101: Shuttle Vectors

    Type
    Blog Post
    Published
    May 20, 2025, 1:15 p.m.
    ...bacterial ones. The mammalian analogue of bacterial transformation is transfection. In stable transfection, DNA...focused this post on shuttle vectors delivered by transformation and transfection and left the business of transduction...Resources on the Addgene blog Plasmids 101: Transformation, Transduction, Bacterial Conjugation, and Transfection...

  10. Lab to Office Culture Shock

    Type
    Blog Post
    Published
    Feb. 5, 2019, 1:04 p.m.
    ...of time that doesn’t involve critical thinking. Former Addgenie Jessica Welch says, “[It] seems counter-intuitive... yourself a Scientist. You work in a lab. You formulate hypotheses and conduct experiments. You transfer...work environments outside of academia provide a formal training regime and the right set of tools to help...

  11. Beyond the Blood Brain Barrier: PHP.B Enters the Central Nervous System Via the LY6A Receptor

    Type
    Blog Post
    Published
    July 23, 2019, 12:46 p.m.
    ... may have contributed to this mouse-specific performance, why LY6A is an unlikely receptor for PHP.B, ...selection of a virus with mouse-strain dependent performance. This surreptitious finding illustrates how the...2019): 538421. If you’re interested in how PHP.B performed in non-human primates, check out these two references...

  12. To Codon Optimize or Not: That is the Question

    Type
    Blog Post
    Published
    Nov. 12, 2020, 2:15 p.m.
    ...protein. Codon optimization could affect protein conformation, folding and stability, change post-translational...  In addition codon optimization could remove information encoded in the primary sequence of a particular...updated resource for codon usage tables. BMC Bioinformatics 18: . https://doi.org/10.1186/s12859-017-1793...

  13. CRISPR Activation: A Practical Guide

    Type
    Blog Post
    Published
    Aug. 18, 2016, 2:30 p.m.
    ...deliver the appropriate isoform(s) of a given gene, as, many times, the isoform(s) common to a particular...Tuttle is a medical student at Tufts University and former researcher at the Wyss Institute for Biologically...

  14. Fluorescent Proteins 101: When GFP lets you down

    Type
    Blog Post
    Published
    Aug. 23, 2018, 12:05 p.m.
    ... GFP is such a well-validated, all-round good performing probe, it is the first choice when selecting ...Zacharias et al., 2002). GFP needs oxygen Chromophore formation requires a fully folded beta-barrel structure,...C-terminal fusions (Adjobo-Hermans, 2011). Structural information facilitates the choice of the proper site, although...

  15. Harnessing Bacterial Toxins for Allelic Exchange

    Type
    Blog Post
    Published
    Aug. 15, 2019, 12:30 p.m.
    ...rely on exogenous enzymes, require multiple transformation and plasmid curing steps, and can generate ... markers for allelic exchange. To do this, we performed the following modifications to pDS132 (Philippe...Hospital where he is now an attending physician. He performs post-doctoral research in the lab of Matthew Waldor...

  16. Five Popular Model Organisms, Part 2

    Type
    Blog Post
    Published
    Sept. 29, 2020, 1:15 p.m.
    ...model in medical research with an archive of information and data that has yet to be produced with mice...Arabidopsis. There are also important tips for plant transformations and the use of Arabidopsis in the classroom...functional genomics study in bacteria with superior performance. Nature Communications, 9(1). https://doi.org...

  17. Using Phosphoserine to Study Protein Phosphorylation

    Type
    Blog Post
    Published
    June 23, 2016, 2:30 p.m.
    ...generate non-phosphorylated recombinant protein and perform in vitro kinase reactions. Often, however, researchers... serves as an intermediate on the path to the formation of cysteinyl-tRNA (4). The discovery of a phosphoserine...PMC4383998. 3. Li, Tong, et al. "Cysteinyl‐tRNA formation: the last puzzle of aminoacyl‐tRNA synthesis."...

  18. Plasmids 101: Common Lab E. coli Strains

    Type
    Blog Post
    Published
    Nov. 7, 2014, 2:56 p.m.
    ...are wild-type unless indicated otherwise fhuA (formerly tonA) ferric hydroxamate uptake, iron uptake receptor...Propagation of ccdB-containing plasmids Hte   High transformation efficiency deoR constitutive expression of...For a more comprehensive list and additional information, visit OpenWetWare's E.coli genotype resource...

  19. A Control for All Seasons

    Type
    Blog Post
    Published
    Oct. 5, 2023, 1:15 p.m.
    ...different types of controls and be able to use this information to design thoughtfully and thoroughly controlled...be due to natural biological variation. This information is critical for correctly interpreting experimental...experimental technique before starting. For more information about antibodies and antibody-based experiments...

  20. Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

    Type
    Blog Post
    Published
    April 5, 2018, 12:42 p.m.
    ...T7 promoter upstream of the left homology arm, perform in vitro transcription, and use the IVT product... and infundibulum. In vivo electroporation is performed immediately after injection. Image from Ohtsuka...contrast to standard microinjection, i-GONAD is performed directly on pregnant female mice using a small...
Showing: 481 - 500 of 756 results