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Showing: 481 - 500 of 766 results

  1. Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins

    Type
    Blog Post
    Published
    March 19, 2019, 1:31 p.m.
    ...the LOV2 domain unfold, disrupting the protein conformation and thus, inhibiting function of Acr. This allows...photoswitchable proteins, which change in protein conformation in response to light, offers a way to spatially...of the photoswitchable cPYP, which changes in conformation in response to blue light (445nm). The discovery...

  2. Meet an Addgenie: Insights into Grad School, Career Paths, and More

    Type
    Blog Post
    Published
    June 25, 2020, 1:15 p.m.
    ...the six months of my co-op, I have conducted informational interviews with nine Addgenies. There are a ...interested to learn more when I was introduced to bioinformatics in the previous programs. I thought, ‘Let me...flexibility and be up to date with the current world. Bioinformatics it is.’” Though she misses the bench, the change...

  3. Overcoming the Challenges of Lentiviral Production

    Type
    Blog Post
    Published
    Aug. 7, 2018, 12:31 p.m.
    ...packaging systems were designed to prevent the formation of replication competent lentivirus (RCL). Replication...requires the viral transactivator, Tat. For more information on the different generations of lentiviral packaging...to obtain the best titer possible. While this information provides a great starting point, it may not translate...

  5. Plasmids 101: Modular Cloning

    Type
    Blog Post
    Published
    March 12, 2024, 1:15 p.m.
    ...cloning strategy designed by synthetic biologists to perform large scale, one-tube cloning reactions. In this...used to assemble multigene constructs capable of performing production and subsequent necessary modification... them to these organisms via another optimized platform. Gene Stacking As the name implies, gene stacking...

  6. Synthetic promoter AAVs for cell-type specific expression in retinal cells

    Type
    Blog Post
    Published
    March 12, 2020, 1:15 p.m.
    ... containing the active promoters at Addgene. Performance of synthetic promoter AAVs in mice All of these...promoter plasmids! Comparing synthetic promoters performance in mouse, monkey, and human retinas Next, the...in monkey and human retinas. By comparing the performance of promoters across these three species, the ...

  7. Open Resources and Plasmid Tools For Studying C. elegans

    Type
    Blog Post
    Published
    July 18, 2019, 12:55 p.m.
    ...CeNGEN is planned for August 15th, 2019. For more information about the aims and approach of CeNGEN, check ... C. elegans. This database provides detailed information about C. elegans 3’UTRs structures, alternative...PMC3531189. Mangone, Marco, et al. "UTRome. org: a platform for 3′ UTR biology in C. elegans." Nucleic acids...

  8. Savvy Advocates Needed to Navigate a Scientific Enterprise in Flux

    Type
    Blog Post
    Published
    Sept. 13, 2016, 2:30 p.m.
    ...symposium includes a panel discussion and keynote by former congressman and CEO of the American Association...bootcamp” for a focused group of participants. More information can be found at http://futureofresearch.org/advocating-for-science-boston...symposium will culminate with a keynote address by former congressman and CEO of the American Association...

  9. Plasmids 101: 5 factors to help you choose the right cloning method

    Type
    Blog Post
    Published
    Aug. 21, 2018, 12:31 p.m.
    ...consider Gibson cloning. In this technique, you first perform a round of PCR to generate DNA fragments with 15...your insert, DpnI digest away the templates, and transform the PCR products into E. coli. Ligases within ...available cloning kits that one could choose from to perform these experiments. In most cases, there will be...

  10. Production of Virus in Insect Versus Mammalian Cells

    Type
    Blog Post
    Published
    Nov. 5, 2024, 8:32 p.m.
    ...understanding the differences can help you make an informed decision. Mammalian cells Mammalian cells are ...Ararat, M., & Graham, F. L. (2002). Preferential transformation of human neuronal cells by human adenoviruses... S., & Ng, S. K. (2021). HEK293 cell line as a platform to produce recombinant proteins and viral vectors...

  11. PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

    Type
    Blog Post
    Published
    June 25, 2024, 1:15 p.m.
    ...thousands of designs in parallel. To do this, we performed high-throughput ‘self-targeting’ screens with ...PRIDICT2.0 score. For the best prime editing performance, we suggest that users select some of the best...learning–based model with high generalization performance. Science Advances, 5(11), eaax9249. https://doi.org...

  12. Academic vs. Industry Postdocs

    Type
    Blog Post
    Published
    Nov. 13, 2018, 1:22 p.m.
    ...project will not involve proprietary compounds or information. Where publication is expected, the process is...ensure that it does not divulge any propriety information. Working as a team: Industry is all about teamwork...at once. Many companies will hire a postdoc to perform a specific project, while others will hire a group...

  13. Experimenting with New Careers while in Grad School

    Type
    Blog Post
    Published
    Feb. 25, 2016, 3:30 p.m.
    ...submitted was hilarious, full of interesting information, and received a lot of attention on social media...Museum and others when I was trying to find local informal science educators, and ever since, the State Museum...the network I built during graduate school is my informal advisory board. Although my goals may not have...

  14. Genome-wide Screening Using CRISPR

    Type
    Blog Post
    Published
    Aug. 18, 2015, 2:30 p.m.
    ...discuss how CRISPR libraries are being used to perform genome-wide screens and highlight some of the reagents...What are the steps involved in a CRISPR screen? Performing a forward genetic screen using CRISPR libraries...your phenotype of interest. For more detailed information on CRISPR screens check out Addgene’s 2016 publication...

  15. Sharing Your Lab Protocols: Using Apps to Save Time & Track Your Experiments

    Type
    Blog Post
    Published
    Sept. 23, 2014, 3:05 p.m.
    ... up after trying out the new protocols.io beta platform for sharing, annotating, and storing life science...associated protocols. When you upload your plasmid information, you’ll have the option to add additional documents...restriction digest of plasmid DNA, and bacterial transformation. And more plasmid-related educational resources...

  16. CRISPR 101: RNA Editing with Cas13

    Type
    Blog Post
    Published
    July 31, 2020, 12:30 p.m.
    ...resulted in 15% C-to-U editing activity. They then performed sixteen rounds of directed evolution across the... cytidine deaminase activity. While RESCUE can perform C-to-U edits, the authors note that it retains ...whole-transcriptome RNA-sequencing, the authors performed rational mutagenesis and identified that a S375A...

  17. To Codon Optimize or Not: That is the Question

    Type
    Blog Post
    Published
    Nov. 12, 2020, 2:15 p.m.
    ...protein. Codon optimization could affect protein conformation, folding and stability, change post-translational...  In addition codon optimization could remove information encoded in the primary sequence of a particular...updated resource for codon usage tables. BMC Bioinformatics 18: . https://doi.org/10.1186/s12859-017-1793...

  18. Plasmids 101: Shuttle Vectors

    Type
    Blog Post
    Published
    May 20, 2025, 1:15 p.m.
    ...bacterial ones. The mammalian analogue of bacterial transformation is transfection. In stable transfection, DNA...focused this post on shuttle vectors delivered by transformation and transfection and left the business of transduction...Resources on the Addgene blog Plasmids 101: Transformation, Transduction, Bacterial Conjugation, and Transfection...

  19. Beyond the Blood Brain Barrier: PHP.B Enters the Central Nervous System Via the LY6A Receptor

    Type
    Blog Post
    Published
    July 23, 2019, 12:46 p.m.
    ... may have contributed to this mouse-specific performance, why LY6A is an unlikely receptor for PHP.B, ...selection of a virus with mouse-strain dependent performance. This surreptitious finding illustrates how the...2019): 538421. If you’re interested in how PHP.B performed in non-human primates, check out these two references...

  20. Lab to Office Culture Shock

    Type
    Blog Post
    Published
    Feb. 5, 2019, 1:04 p.m.
    ...of time that doesn’t involve critical thinking. Former Addgenie Jessica Welch says, “[It] seems counter-intuitive... yourself a Scientist. You work in a lab. You formulate hypotheses and conduct experiments. You transfer...work environments outside of academia provide a formal training regime and the right set of tools to help...
Showing: 481 - 500 of 766 results