We narrowed to 515 results for: gan
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TypeBlog PostPublishedJuly 14, 2022, 1:15 p.m.... collection of scripts evolved into a single, organized pipeline and accelerated the processing of our...
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Antibodies 101: Reading a Flow Plot
TypeBlog PostPublishedFeb. 6, 2024, 3:13 p.m....Vegas, A., Ma, M., Sahay, G., Chiu, A., Bader, A., Langan, E., Siebert, S., Li, J., Greiner, D., & Anderson... -
Hot Plasmids Spring 2024
TypeBlog PostPublishedMay 23, 2024, 1:15 p.m....vitro and in vivo, including in human retinal organoids and live mice. Importantly, REVeRT avoids the ... -
Viral Vectors 101: Pseudotyping
TypeBlog PostPublishedApril 20, 2021, 2:30 p.m....more safely. Recently, many SARS-CoV-2 researchers began pseudotyping the SARS-CoV-2 spike protein into a... -
CRISPR-based Genome Editing Systems For Editing in E. coli
TypeBlog PostPublishedApril 13, 2021, 1:15 p.m....on the escape mechanism of genome editing in microorganisms which could be applied to optimization of genome... -
3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells
TypeBlog PostPublishedSept. 5, 2017, 1:58 p.m....versatile tool for precise gene editing in many organisms and model systems. We have used CRISPR/Cas9 extensively... -
Deep Mutational Scanning with One Pot Saturation Mutagenesis
TypeBlog PostPublishedFeb. 22, 2017, 3:30 p.m....phenotype that can be used with mutational scanning: ligand binding assays, phage display assays, ubiquitination... -
Multiple Plasmids at a Low Price: Kits from Addgene
TypeBlog PostPublishedSept. 14, 2016, 2:30 p.m....Sciences 108.38 (2011): 15870-15875. See Kendall Morgan’s blog post on the PUF Assembly Kit for some insights... -
10 Steps to a Perfect Science Talk
TypeBlog PostPublishedAug. 23, 2016, 2:30 p.m....Speaking longer just makes you seem unprepared or arrogant. A bit short is always better than too long, and... -
Evolution of Lab Techniques
TypeBlog PostPublishedJune 21, 2016, 2:30 p.m.... abundance. If a scientist wants to know if an organism has a particular gene mutation, southern blotting... -
Harnessing Bacterial Toxins for Allelic Exchange
TypeBlog PostPublishedAug. 15, 2019, 12:30 p.m....s toolbox. Depending on the growth rate of the organism of interest, from the day primers arrive, the ... -
Viral Vectors 101: Integrase-Deficient Lentiviral Vectors
TypeBlog PostPublishedMay 13, 2025, 1:15 p.m....indispensable tools for manipulating cells and organisms. They can be used for a variety of research purposes... -
Hot Plasmids: Summer 2024
TypeBlog PostPublishedAug. 27, 2024, 1:15 p.m....science.ado7082. Nuñez, J. K., Chen, J., Pommier, G. C., Cogan, J. Z., Replogle, J. M., Adriaens, C., Ramadoss,... -
CRISPR 101: Homology Directed Repair
TypeBlog PostPublishedJan. 26, 2023, 2:15 p.m....). PubMed. Making designer mutants in model organisms. Peng, Y. et al. Development 141; (2014). PubMed... -
How to Negotiate a Successful Lab Start Up Budget
TypeBlog PostPublishedSept. 1, 2015, 2:30 p.m....provide value to others This is a non-profit organization. Therefore, collaboration and community is the... -
Plasmids 101: Common Lab E. coli Strains
TypeBlog PostPublishedNov. 7, 2014, 2:56 p.m....blog post covering E. coli and others are model organisms! Our Most Popular Plasmids 101 Posts: Origin... -
Is this the right place for me? 8 tactics for choosing a lab
TypeBlog PostPublishedOct. 2, 2018, 12:56 p.m....encourage diversity in all forms? How are their organizational skills? Are they involved with colleagues ... -
Easi-CRISPR: Generating Knock-In and Conditional Mouse Models
TypeBlog PostPublishedApril 5, 2018, 12:42 p.m....made targeted insertions relatively simple in C. elegans and bacteria. But CRISPRing typical mouse models... -
Generating Mouse Models Using CRISPR/Cas9
TypeBlog PostPublishedJuly 12, 2016, 2:30 p.m....0471142905.hg1508s88 Leidy-Davis T, Cheng K, Goodwin LO, Morgan JL, Juan WC, Roca X, Ong ST, Bergstrom DE (2018... -
CRISPR 101: Epigenetics and Editing the Epigenome
TypeBlog PostPublishedJune 24, 2020, 5:45 p.m....a number of tools for this purpose. Epigenetics began as a correlative field in which covalent modifications...