We narrowed to 815 results for: lars;
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TypeBlog PostPublishedAug. 28, 2018, 12:04 p.m....microscopes work as conventional microscopes and give a similar optical quality (magnification of 140X and 2 micron... to microbes by using non-invasive methods for cellular imaging with ultrasound. In his research he applies...applies naturally occurring bacterial “molecular buoyancy devices” (also known as gas vesicles) as acoustic...
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Measuring Kinase Activity at the Single-Cell Level with Kinase Translocation Reporters (KTRs)
TypeBlog PostPublishedJuly 26, 2018, 12:46 p.m....phosphorylated on at least one site. Phosphorylation is particularly important for regulating signal transduction...their Kinase Translocation Reporters (KTRs) whose cellular localization serves as a proxy measurement of ... used to measure kinase activity in specific subcellular compartments. Second, because KTRs shuttle between... -
Fluorescent Proteins 101: History of Fluorescent Proteins
TypeBlog PostPublishedAug. 7, 2017, 1:58 p.m....like GFP came to be used as research tools is particularly interesting. Although humans have known about...crystal jellyfish Aequorea victoria (Figure 1) in molecular detail. On the underside of the “umbrella” of ...Soon, the number of people studying it grew. In particular, studies in the laboratory of UCSD biochemist... -
Designer PUF Proteins for Any RNA Target
TypeBlog PostPublishedJune 17, 2014, 7:57 p.m....Urbana-Champaign. The PUF proteins include eight modular repeats, each of which binds a consecutive base...on a version that she hopes will change the intracellular localization of RNA. “You can think of changing...the kit.” Reference Abil Z, Denard CA, Zhao H. Modular assembly of designer PUF proteins for specific ... -
A new platform for fast functional characterization of potentially cancerous mutations
TypeBlog PostPublishedMay 7, 2019, 12:39 p.m.... responsible for cancer from genomics data, and large-scale patient cohort data from the MD Anderson Cancer...constructs using the High-Throughput Mutagenesis and Molecular Barcoding (HiTMMoB) technique (Tsang et al., 2016... perturbations in vitro using Ba/F3 and MCF10A cellular models. Ba/F3 and MCF10A are growth factor dependent... -
Phage Directory: From Phage Therapy to a Repository of Phage Information
TypeBlog PostPublishedJan. 10, 2019, 2:38 p.m....correct phages will test. These includes labs with a large number of different phages or with phages targeting...that phage information was very fragmented. A particular phage researcher may only know how to prepare...networking Additional resources on Addgene.org Find molecular biology protocols here Visit the Addgene careers... -
CRISPR Protocol for Genomic Deletions in Mammalian Cell Lines [Video]
TypeBlog PostPublishedFeb. 18, 2015, 3:09 p.m....production of deletions by paired DSBs may be particularly useful. The first is the study of non-coding...and Harvard Medical School. His lab studies the molecular genetics of blood cell development and disease...genomic deletion efficiency mediated by clustered regularly interspaced palindromic repeats (CRISPR)/Cas9 ... -
IBEX Knowledge-Base: A Data Resource for Multiplex Tissue Imaging
TypeBlog PostPublishedJune 8, 2023, 1:15 p.m.... Affinity Reagent Working Group of the Human Biomolecular Atlas Program (HuBMAP) antibody metadata standards.... Users can also recommend or not recommend a particular reagent in the given conditions, along with the...building both the community and the database. They particularly encourage people to upload and share their negative... -
Who Gives a Tweet? 9 Facts About Scientists on Twitter
TypeBlog PostPublishedJune 26, 2017, 1:31 p.m....Instagram Social media and news sites were also popular. Perhaps scientists, like nonscientists, blur the...the more friends they have in common. Only the largest connected part of the follower network is shown...physical (blue) and social (orange) scientists largely cluster by discipline, suggesting that scientists... -
6 Steps to Submitting a Resume That Gets Seen
TypeBlog PostPublishedJuly 9, 2015, 2:30 p.m....excellent resume. 2) Follow job boards and check them regularly Use your research skills to determine which job... to take next steps and follow up especially in large companies. It is fine to address the cover letter...might be able to help you. If the organization is large, you will need to be more directed and target someone... -
9 Tips to Achieve Success in Academia
TypeBlog PostPublishedApril 1, 2014, 5:33 p.m....follow their passion. “There are many ways to have a large positive impact in society. We need much, much more...studies. They suggest that you make time for extracurricular activities both to develop new skills and to...information about the benefits of pursuing extracurricular activities. Success evolves from passion True... -
Five Questions to Ask If You're Deciding Between a Master's or PhD Program
TypeBlog PostPublishedFeb. 16, 2021, 2:15 p.m....scientist, or so I thought. So I entered a molecular and cellular biology PhD program in 2011, which I left...different however. In industry the career ladder is larger and there are more opportunities for promotion.... -
Tips for Improving Your Next Manuscript
TypeBlog PostPublishedNov. 22, 2016, 3:30 p.m....welcoming us. Then it was time to work! Set up similarly to the online class, we met as a group for sessions...colleagues’ manuscripts and discuss changes to particular sections. The focus groups allowed us to try ...try out techniques that were discussed in the larger sessions and reinforced our newfound knowledge with... -
Bringing Sustainable Practices to the Lab: Recycling
TypeBlog PostPublishedFeb. 14, 2019, 2:35 p.m....Anyone who works in a lab is familiar with the alarming amount of waste that is generated. This is a systemic...first post will be based on recycling! With the popularity of single-use items, plastic is a substantial... still be interested as it offsets the cost of regular waste services. Learn about how to start a glove... -
Addgene-Seeding Labs Plasmid Grant: Accelerating Science Globally - 5 Scientists, 4 Countries & 3 Continents!
TypeBlog PostPublishedJuly 18, 2017, 12:41 p.m....Robertson (far left) is a Senior Lecturer and a Plant Molecular Biology and Biotechnology expert. In order to ...engineer plants that are able to destroy DNA viruses, similar to the anti-phage CRISPR effect observed in bacteria...Nair, Associate Professor (Right), focuses on molecular biotechnology for improving expression of nutritionally... -
Plasmids 101: Positive and Negative Selection for Plasmid Cloning
TypeBlog PostPublishedAug. 22, 2019, 12:43 p.m....plasmids at Addgene Read our molecular biology reference Watch molecular biology protocol videos ...that have lost the sacB gene. SacB can be used similarly to CcdB. For example, in the gene expression plasmid... -
Networking and Inspiring Young Scientists at Freshers Fairs
TypeBlog PostPublishedNov. 17, 2015, 3:30 p.m.... events are one of the main ways budding young scholars across Europe get introduced to the cultures and...collaborations and conferences for their students. Another large goal of the organisation is to encourage iGEM alumni...these biological societies engaging with young scholars. We believe it’s very important to develop a network... -
Multifunctional Neural Fibers: Modulation from Gut to Brain
TypeBlog PostPublishedAug. 31, 2023, 1 p.m....undeniably difficult connection to study on a molecular level. The brain is constantly influenced by signals...organisms, including the Anikeeva lab’s work on a similar neural fiber (Park et al., 2019). This provided...., Anikeeva, P., & Chandrakasan, A. P. (2020). Modular Optoelectronic System for Wireless, Programmable... -
Addgene Receives NIH BRAIN Initiative Grant to Create Open-Access Recombinant Antibody Resource
TypeBlog PostPublishedMay 25, 2021, 2:29 p.m.... including more secure archiving, unambiguous molecular definition, and potential for further engineering...is a critical unmet need for an open-access, molecularly defined, and well validated collection. I’m thrilled..., Wong J, Koh XY, Eng H-Y, Lane D, Lefranc M-P, Clark M, Dübel S (2018) When monoclonal antibodies are... -
Viral Genome Sequencing - A One Stop Shop for AAV Identity and Serotype Confirmation
TypeBlog PostPublishedApril 23, 2020, 1:15 p.m....but for viral vector core facilities producing a large number of viral preparations in parallel, there ...Single-stranded Virus Next-generation Sequencing. Molecular Therapy - Nucleic Acids 4:e260 . https://doi.org...Batch-to-Batch Consistency Evaluation of AAV Preparations. Molecular Therapy 25:1375–1386 . https://doi.org/10.1016...