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...essentially any gene,” she says. The technical leap from mutating and deactivating a gene or genes to selectively...that the SAM system is robust, specific, and can facilitate genome-scale gain-of-function screening when ...

...excited to see how this exchange of genetic tools facilitates plant science. Challenge #3: Intellectual property...in 2004, she accepted a commission in the United States Air Force. She left active duty to pursue a degree...

...Assign each meeting a leader from the group in a rotation. The leader for that meeting is responsible for...and meet to debrief on the topic (career) or presentation style (science). Have a potluck dinner. Invite...

... in the orientation of the two fragments. However, rigidity can be introduced through mutation, allowing...

...sometimes assumed that the direct method is more quantitative than the indirect method. But in reality, multiple...immunoimaging assays do not allow for absolute quantitative analysis of a sample. Instead, relative quantification...

...routinely fused to ‘tag’ or ‘label’ proteins to facilitate its detection. Fluorescent proteins are most ...chemogenetic receptor. This may not be an important limitation in many studies, and could even be beneficial...

...selective small molecule binders. To overcome this limitation, we created a generalizable tag-based strategy...context. Immunoblotting is recommended, while quantitative proteomics experiments will provide the best...

...RANbody platform from the Sanes Lab overcomes this limitation and allows for the flexible design and small ...you own DNA ladders with these plasmids from Penn State Need help picking the right fluorescence microscopy...

...and involved presentations and a handout on protocol sharing, material sharing, computational reproducibility...

...We are delighted at the early outcomes and the ecstatic reception by scientists. The success of these...candidates from internal referrals. Our long reaching reputation helps us hire great people from farther away ...

...therapeutic delivery by AAV, which has strict capacity limitations. New efforts have recently been made to increase...engineering goals expand beyond generating frameshift mutations or single base pair edits, these enzymes are worth...

...plasmid. Each gRNA combination can be tracked and quantitatively analyzed by sequencing its set of barcodes....: Forward oligo: 5’- CACCGNNNNNNNNNNNNNNNNNNNGTTTGGGTCTTCGAGAAGACCTATTCXXXXXXXXC -3’; Reverse oligo: 5...

...at this event added to a growing dialogue on the state of a system that, despite all of its successes, ...Andrew Young School of Policy Studies at Georgia State University, is an expert on the academic labour ...

...the take rate of mouse xenografts after cell implantation. Their goal was to figure out how to optimize...lung cancer cell line containing an activating mutation in epidermal growth factor receptor (EGFR) with...

... that flank their DNA fragment in an inverse orientation. This allows for multiple DNA components (promoters...amplification can be used to create silent point mutations at internal recognition site(s) thus eliminating...

...genetic engineering on a genomic scale, with the expectation for major advances in agriculture, materials,...could fix these things, then I think there is no limitation regarding the size of the networks you could ...

...sense to get my master’s and leave. I was really devastated when that happened. I didn't know if I should.... The choice to not go to medical school was devastating. At the same time, she has an entirely new path...

...MuLE (Multiple Lentiviral Expression) system facilitates the simultaneous introduction of multiple genetic...overexpression, knockdown (shRNA, miR-30-shRNA), mutation/editing (CRISPR-Cas9) or deletion (Cre), together...

...paper, but positive outcomes can include poster presentations, talks about your work, or even the successful...Learn How to Become a Grant Writing Professional Facilitate Career Planning and Personal Development with...

...scientific articles, patents, and even some doctoral dissertations. Streamline the question asking process Having...answer, consider asking to avoid unrealistic expectations. And finally, provide feedback. If you are...