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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 718 results for: tat

Showing: 481 - 500 of 718 results

  1. Form Your Own Peer Mentoring Group: A How-To Guide for Scientists

    Type
    Blog Post
    Published
    Feb. 18, 2014, 4:22 p.m.
    ...Assign each meeting a leader from the group in a rotation. The leader for that meeting is responsible for...and meet to debrief on the topic (career) or presentation style (science). Have a potluck dinner. Invite...

  2. dTAG - You're it!

    Type
    Blog Post
    Published
    June 21, 2018, 2:06 p.m.
    ...selective small molecule binders. To overcome this limitation, we created a generalizable tag-based strategy...context. Immunoblotting is recommended, while quantitative proteomics experiments will provide the best...

  3. RANbodies: Reporter Nanobody Fusions

    Type
    Blog Post
    Published
    April 10, 2018, 12:56 p.m.
    ...RANbody platform from the Sanes Lab overcomes this limitation and allows for the flexible design and small ...you own DNA ladders with these plasmids from Penn State Need help picking the right fluorescence microscopy...

  5. Celebrating 15 Years of Scientific Sharing

    Type
    Blog Post
    Published
    Jan. 14, 2019, 2:08 p.m.
    ...We are delighted at the early outcomes and the ecstatic reception by scientists. The success of these...candidates from internal referrals. Our long reaching reputation helps us hire great people from farther away ...

  6. Antibodies 101: Affinity Reagents

    Type
    Blog Post
    Published
    April 14, 2022, 1:15 p.m.
    ... in the orientation of the two fragments. However, rigidity can be introduced through mutation, allowing...

  7. Antibodies 101: Secondary Antibodies

    Type
    Blog Post
    Published
    Dec. 21, 2021, 2:15 p.m.
    ...sometimes assumed that the direct method is more quantitative than the indirect method. But in reality, multiple...immunoimaging assays do not allow for absolute quantitative analysis of a sample. Instead, relative quantification...

  8. CRISPR 101: Cas9 vs. The Other Cas(s)

    Type
    Blog Post
    Published
    Dec. 8, 2022, 2:15 p.m.
    ...therapeutic delivery by AAV, which has strict capacity limitations. New efforts have recently been made to increase...engineering goals expand beyond generating frameshift mutations or single base pair edits, these enzymes are worth...

  10. Pairing CombiGEM and CRISPR for Combinatorial Genetic Screening

    Type
    Blog Post
    Published
    April 12, 2016, 2:30 p.m.
    ...plasmid. Each gRNA combination can be tracked and quantitatively analyzed by sequencing its set of barcodes....: Forward oligo: 5’- CACCGNNNNNNNNNNNNNNNNNNNGTTTGGGTCTTCGAGAAGACCTATTCXXXXXXXXC -3’; Reverse oligo: 5...

  11. The Future of Research Symposium Boston 2015

    Type
    Blog Post
    Published
    Oct. 20, 2015, 2:30 p.m.
    ...at this event added to a growing dialogue on the state of a system that, despite all of its successes, ...Andrew Young School of Policy Studies at Georgia State University, is an expert on the academic labour ...

  12. New Tool for Lineage Tracing: The ClonTracer Library

    Type
    Blog Post
    Published
    Sept. 22, 2015, 2:30 p.m.
    ...the take rate of mouse xenografts after cell implantation. Their goal was to figure out how to optimize...lung cancer cell line containing an activating mutation in epidermal growth factor receptor (EGFR) with...

  13. Plasmids 101: Golden Gate Cloning

    Type
    Blog Post
    Published
    Aug. 27, 2015, 2:30 p.m.
    ... that flank their DNA fragment in an inverse orientation. This allows for multiple DNA components (promoters...amplification can be used to create silent point mutations at internal recognition site(s) thus eliminating...

  15. Popular Retroviral Vectors and Their Uses in Scientific Research

    Type
    Blog Post
    Published
    July 24, 2018, 12:51 p.m.
    ...MuLE (Multiple Lentiviral Expression) system facilitates the simultaneous introduction of multiple genetic...overexpression, knockdown (shRNA, miR-30-shRNA), mutation/editing (CRISPR-Cas9) or deletion (Cre), together...

  16. Meet an Addgenie: Insights into Grad School, Career Paths, and More

    Type
    Blog Post
    Published
    June 25, 2020, 1:15 p.m.
    ...sense to get my master’s and leave. I was really devastated when that happened. I didn't know if I should.... The choice to not go to medical school was devastating. At the same time, she has an entirely new path...

  17. 6 Tips for Grant Writing

    Type
    Blog Post
    Published
    Oct. 18, 2016, 2:30 p.m.
    ...paper, but positive outcomes can include poster presentations, talks about your work, or even the successful...Learn How to Become a Grant Writing Professional Facilitate Career Planning and Personal Development with...

  18. Tips for Technical Support Calls

    Type
    Blog Post
    Published
    Sept. 20, 2016, 2:39 p.m.
    ...scientific articles, patents, and even some doctoral dissertations. Streamline the question asking process Having...answer, consider asking to avoid unrealistic expectations. And finally, provide feedback. If you are...

  19. Plasmids 101: Protein Expression

    Type
    Blog Post
    Published
    June 7, 2018, 1:17 p.m.
    ..., tobacco, rice, sugarcane, and even tubers of potatoes have been used for protein expression. Plant ...well-defined media and can be easily adapted to fermentation allowing for large-scale, stable production ...

  20. xCas9: Engineering a CRISPR Variant with PAM Flexibility

    Type
    Blog Post
    Published
    March 28, 2018, 6:52 p.m.
    ... Base editing, another method to create point mutations, also has a strict editing window about 15 nt ...mutagenesis plasmid (MP6) permits quick, large-scale mutation of the gene of interest. They created a selection...
Showing: 481 - 500 of 718 results