We narrowed to 904 results for: CAN

... you can do this. If you are going to complete your colony PCR analysis in the same day, you can save ... on an LB plate. You can use this plate to start liquid cultures. Lastly, you can start small overnight...with restriction enzyme digest; however colony PCR can accomplish the same thing in less time and for less...clone without the insert. This type of primer pair can tell you if the insert is the correct size and whether...the size of the insert and if it's in the plasmid Can be used to screen clones that were created with the...Taq polymerase is sufficient. Controls: Controls can make or break an experiment. The best controls for... insert. These controls are quick references you can use when you run your PCR products out on a gel to...

...: Cut: CRISPR can be used to cut the DNA sequence. With a repair template, CRISPR can be used to introduce...100% specific and can cut DNA sequences that researchers don’t intend it to. CRISPR cannot cut all sequences...DNA. Fusing the nuclease to an adenosine deaminase can convert adenosine to inosine. Activate/Repress: Modified...proteins continue to expand the targeting range. CRISPR can be difficult to deliver. Not all cells efficiently... Modified versions of CRISPR nucleases that can’t cut DNA (dCas9, for example) that are used to increase...

...function downstream of DNA, such as RNA interference, can reversibly exert their effect independent of gene...of different small RNAs (Figure 1). These siRNAs can exert their gene silencing effect through targeted...sequences (Figure 1). As a result, gene silencing can be easily induced by exogenous application of long...machinery. Alternatively, endogenous miRNA precursors can be engineered to created artificial miRNAs. The processed...CRISPR-generated mutants believed to be knockouts can maintain partial function due to the use of exon ...trigger RNAi activation. A typical RNAi experiment can be divided into three fundamental steps: creation... (Figure 1). With this technique, multiple genes can be simultaneously silenced if a consensus sequence...

...Overexpression methods flood the cell with protein, which can both interfere with a cell’s normal function and ...to ensure the edited cells are behaving normally can be prohibitively expensive for many labs. We’ve created...information and images generated from these cell lines can be found in the Cell Catalog on our data portal. ...components to physically enter the cells so they can go to work. Figure 3: Inducing the double ...Cell Catalog on the Allen Cell Explorer, where you can also find the certificate of analysis provided with... The editing strategy and design described above can be used to create similar donor plasmids for introducing...

...vaccine design. We all want tools that expand what we can achieve, but are simple to pick up and work well ...cell? By analogy to Click Chemistry, Click Biology can be defined as a set of reactions derived from the...throughout living organisms, Click Biology reactions can add new functionality to existing genes, while being...dialing up the right nucleic acid sequence (Addgene can help with this!). Besides requiring stable covalent... are oxidized in disulfide bonds. Reactions also can happen at both body temperature and on ice, when ...There are also SNAP-tag and HaloTag modules, which can connect proteins to any kind of small molecule probes...degradation or electrophile signaling. Many of these tools can be found in Addgene's repository (Table 1). ...

...that can impact reproduction include embryonic lethality, a short lifespan, or infertility and can arise...modeling blog series, we covered techniques that can be used to introduce genetic modifications into mouse...growing colony of genetically engineered mice, what can you do? In this post, we’ll cover why and how to ...transgene in order to see an effect. Hemizygous mice can be included in breeding strategies identical to those...mutation at one, but not both, of the alleles. You can cross homozygous or heterozygous mice from your F1... of integration of the transgene. These problems can be mitigated in different ways. Prior to engineering...transgene. In existing strains of mice, the transgene can be maintained on a hemizygous or heterozygous background...

... subtype in the brain that can either activate or inhibit neurons. You can find information more about...inflammation, and neurotransmission. These receptors can be activated by an array of different ligands, including...signaling  So with the hundreds of GPCRs out there, how can scientists decipher the signaling pathways from ligand...the transcription factor from the receptor, which can then activate expression of a luciferase reporter...potential to discover new ligands for unknown GPCRs. You can find the entire PRESTO-TANGO kit at Addgene! TRUPATH...the 16 non-visual heterotrimeric G proteins that can be activated downstream of GPCRs. This collection...subunits of the heterotrimeric G protein complex that can be used to detect G protein activation via bioluminescence...

...is highly flexible and can accommodate any genetic elements of interest. It can thus be tailored to address... Alan Wong. The complexity of biological systems can hinder our attempts to study and engineer them, but...specific genetic perturbations. The ease with which one can design and synthesize CRISPR guide RNAs (gRNAs) for...provides a means to create barcoded gRNA libraries that can be used to combinatorially modify the genome, screen...different gRNAs in each plasmid. Each gRNA combination can be tracked and quantitatively analyzed by sequencing... With a list of gRNA targeting sequences, you can then readily generate your barcoded gRNA library ...interrogate and understand complex biological systems. He can be contacted at [email protected].   References 1. Cheng...

...The computer scientists can get involved in modelling, the electronics folks can build a robot worm on ...meaning that we can obtain known mutants at $7.00 a strain. Makerspaces like ours can also make accounts...technologies and spaces where interested parties can do research. This interest manifested itself in the...on top of that model, and the biologists can get involved a in variety of aspects from the molecular biology...but that would be useful to our model  so that we can use CRISPR/cas9 to build the mutants, analyse their...intend to focus on motor neuron knockouts so that we can perturb factors related to worm motion. Working on... area, please stop by. And if not, I suspect you can find a makerspace somewhere near you. The place to...

...sensitivity, which can be problematic if you are trying to show that your antibody can distinguish between...results so that others in the community can benefit and we can all help improve antibody reproducibility...antibody has been appropriately validated so that you can have confidence in your results. But what is appropriately...that you receive; citing existing validation data can be acceptable. However, it is your responsibility...for the target antigen - Just because an antibody can bind to its target, does not mean that is all it ...represent five experimental strategies researchers can use to show that their antibody performs as expected... types are amenable to genetic manipulation, nor can all genes be easily knocked down or out.   Example...