We narrowed to 614 results for: CaS;

...fluorophore-conjugated antibody commercially available. In other cases, fluorophore-conjugated antibodies may exist, but...may want to look at other methods. In the above cases, a good alternative is using a two-protein detection... antibodies that block surface proteins. In that case, you would want to look at negative selection: labeling...+ T cells, B cells, and dendritic cells. In this case, we simply need to label the lineage markers of ... a separate fix/perm cytosolic procedure in that case.     Figure 3: For intracellular labeling...

... Today on the Addgene Podcast, we have another interview conducted by European Outreach Scientist Benoit... Listen to the interview with Joachim Goedhart Podcast breakdown Intro Chapter 1 - How Joachim ended up...the audio engineer and producer for the Addgene Podcast.      Additional Resources from the Addgene Blog... addgene podcast...

...still be taken when using them. There are suspected cases of insertional mutagenesis in humans (1) and AAVs...Currently, AAVs are being used to deliver CRISPR/Cas9 but have the potential to deliver other therapeutic...used with new exciting technologies (such as CRISPR/Cas9) as they allow for gene delivery in an efficient... 24086725. PubMed Central PMCID: PMC3785459. 4. Castle, Michael J., et al. "Adeno-associated virus serotypes...Expression in Mammals A Match Made in Heaven: CRISPR/Cas9 and AAV Viral Vector Elements Additional Resources...

...Today’s episode of the Addgene Podcast features guest host and Addgene European Outreach Scientist, Benoit...researcher. Listen to the interview with Tom Ellis Podcast breakdown Intro: How Tom ended up in academia and...the audio engineer and producer for the Addgene Podcast.      Additional Resources from the Addgene Blog... addgene podcast...

...used for each gene. At the moment, 13 full genome SpCas9 knockout libraries designed by different academic...Library, for example related to strategies beyond SpCas9, or species not currently included in Green Listed...libraries. As of now I have focused on human and mouse SpCas9 knockout libraries, but there are of course great...activity and minimize off-target effects of CRISPR-Cas9." Nature biotechnology 34.2 (2016): 184-191. PubMed...PMC5445660. 7. Chari, Raj, et al. "Unraveling CRISPR-Cas9 genome engineering parameters via a library-on-library...

...Optogenetic Tools Like pDawn  Making cassava plants resistant to cassava mosaic virus Dr. Fiona Robertson,...and Biotechnology expert. In order to preserve Cassava yields and to increase the potential for this drought...plant, Dr. Robertson aims to make use of the CRISPR/Cas9 technology to engineer plants that are able to destroy...

...including several pooled libraries: Cas13X and Cas13Y, two compact Cas13 proteins found in the metagenome...end-point sequencing assays. These plasmids use “Cas9-mediated adenosine transient reporter for editing...

...preps for the delivery of Cas9 endonuclease, Cas9 nickase, Cas9 activators, Cas9 repressors, select gRNAs...

...repository use Cas9 to cut DNA, Addgene Science Communications Intern Mary Gearing explains how Cas9 homolog,... suggestions. 8. A Match Made in Heaven: CRISPR/Cas9 and AAV Adeno-associated viral (AAV) vectors are...Validating Your Genome Edit Once you’ve used CRISPR/Cas9 to make a change to the genome, you need to check...

...pluripotent stem cell (iPS) technology and CRISPR-Cas9 constructs from Addgene to gene-edit mutations into...looking at a bunch of different strategies with CRISPR-Cas9. There are so many different variants and strategies... of those mutations, they now plan to use CRISPR-Cas9 technology to create cell lines and/or mouse models...ve proposed to use plasmids with modified nickase Cas9 protein in order to target the specific gene region...