...Optogenetic Tools Like pDawn  Making cassava plants resistant to cassava mosaic virus Dr. Fiona Robertson,...and Biotechnology expert. In order to preserve Cassava yields and to increase the potential for this drought...plant, Dr. Robertson aims to make use of the CRISPR/Cas9 technology to engineer plants that are able to destroy...

...preps for the delivery of Cas9 endonuclease, Cas9 nickase, Cas9 activators, Cas9 repressors, select gRNAs...

...including several pooled libraries: Cas13X and Cas13Y, two compact Cas13 proteins found in the metagenome...end-point sequencing assays. These plasmids use “Cas9-mediated adenosine transient reporter for editing...

...repository use Cas9 to cut DNA, Addgene Science Communications Intern Mary Gearing explains how Cas9 homolog,... suggestions. 8. A Match Made in Heaven: CRISPR/Cas9 and AAV Adeno-associated viral (AAV) vectors are...Validating Your Genome Edit Once you’ve used CRISPR/Cas9 to make a change to the genome, you need to check...

...pluripotent stem cell (iPS) technology and CRISPR-Cas9 constructs from Addgene to gene-edit mutations into...looking at a bunch of different strategies with CRISPR-Cas9. There are so many different variants and strategies... of those mutations, they now plan to use CRISPR-Cas9 technology to create cell lines and/or mouse models...ve proposed to use plasmids with modified nickase Cas9 protein in order to target the specific gene region...

...targeting, with a leg up on the alternatives - RNAi and Cas13. CRISPR-Csm doesn’t degrade proximal RNAs nor does...levels of off-target effects, both of happen with Cas13. CRISPR-Csm degrades both nuclear and cytoplasmic...nuclear-RNA targeting, where siRNAs are ineffective and Cas13 has steep off-target costs. Additionally, this system...imaging tool.   Fig. 1: Csm, shRNA, and Cas13 were compared head-to-head for gene expression changes...

...Producing yeast expression cassettes Traditionally, generating expression cassettes for yeast transformation...Cloning sgRNAs Production of Yeast Expression Cassettes Generating constructs with multiple point mutations...regions of homology and inserted into a linearized cassette, ready for transformation into yeast that same...

...Scientists SHOULD Be Using Twitter" podcast from Hello PhD In this podcast the hosts, Josh and Dan, discuss...monitoring that conference via social media. The podcast also features an interview with Dr. Stephani Page...and offers for postdoc positions. Check out the podcast for more tips on using Twitter as a scientist. ...

... absence of a protein, a yes-no plot is the best-case result. For example, you have introduced a GFP protein... not be as obvious in a dot plot. If that is the case for you, switching to a contour plot should indicate...interest. Created with biorender.com.   In such cases, instead of trying to quantify based on percentage...a good statistic to compare between samples. In cases where there is no clear distinction between the ...

.... Expert reviews on blog posts are done on a case-by-case basis, as many of our blog contributors primarily...webinars, or use other research methods. In the case of the antibody guide, primary research and previously...changes and require updating or retiring. CRISPR/Cas9, for example, has gone through rapid change in the...