...from that of the gene of interest, or expressed from the same transcript as the gene of interest, but ...visualize when and where the gene is expressed. Click here to view Addgene’s collection of empty backbones... a wide number of functions, including: tagging genes for elucidating their expression or localization...promoter of interest can be used to effectively monitor gene expression from that promoter in a given cell type...more about optogenetics at OpenOptogenetics and find optogeneic actuators and sensors at Addgene. Cell marking...and others. Review Addgene's collection of fluorescent biosensors here. Optogenetics: Scientists can use...implantation and generation of transgenic mice. Purification: GFP can be used as a general epitope tag for...

...dealing with a defective gene, Xm, the sequence of which is identical to the correct gene, Xw, except for a ...Adenine base editors (ABE) What if you have another gene Y, which has a defective allele Ym and you need ...first created a defective chloramphenicol resistance gene (CamR) by introducing a point mutation (H193Y). ...transformed it into E. coli harboring the defective CamR gene. Screening for surviving colonies and subsequent...system. We use a single polycistronic tRNA-gRNA (PTG) gene system to clone multiple protospacers which enables... slim chance of generating indels with base editors, keep in mind that the generation of indel sometimes...biology Resources on Addgene.org Find all CRISPR plasmids and resources at Addgene Find CRISPR base editors...

... popular tools for genetic screens, enabling genome-wide interrogation of the genes required for cellular...that constitutively express Cas9 and screen for genes that affect Th2 differentiation and activation, ...inserting the NanoLuc luciferase downstream of the apoB gene. Using this reporter, the researchers explore many...-LPs and accelerate the discovery of additional genes and small-molecule probes to aid in the understanding...transduction, now enabling forward genetic screens in cells with few genetic tools. Find the mouse CRISPR ...plasmids at Addgene. Liu et al., Nature Communications, 2019. PubMed PMID: 31451697. Genetically encoded ...responsible for a phenotype can be identified by next-generation sequencing of the genomic DNA. However, some ...

...for its powerful promoter of a gene important for methanol usage. This gene promoter, AOX1, was co-opted...experimental model. In fact, approximately 25% of yeast genes have a human counterpart, enabling study of human-relevant...Saccharomyces cerevisiae to human: The important gene co‑expression modules. Biomedical Reports 7.2 (2017...fast (doubling time of ~ 2 hours) Amenable to genetic manipulation The first eukaryote (S. cerevisiae... new at all, but it’s not as well-known in the general research community. Phillips Petroleum Company...release the K. phaffii expression system to the general research community in the 1990s when they abandoned...the strain tractable for peroxisomal studies on biogenesis, degradation, and proliferation. Genome manipulation...

... for gene expression, turning transcription on and off by binding proximal to their target genes. Since...Since gene expression patterns determine everything from stem cell differentiation fate to tumor suppression...from the library to further investigate specific genes and TF isoforms. Due to the size of the collection...use to generate the relevant disease model. Accurate modeling of a disease depends on both genetic and expression... not yet available, just ask an Addgene scientist at help@addgene.org! References and Resources  References...scientific community. Recently, the Zhang lab generated a TF library (aka the MORF Collection) and an ...isoforms transcribed from these sites. The Zhang lab generated a comprehensive library of each unique TF open...

...ampicillin resistance gene, you’ll find the full name of the gene, the name of its gene product, and a description... plasmid features such as antibiotic resistance genes, selectable markers, promoters, origins, tags, and... Overview of SnapGene powered plasmid maps at Addgene  Three factors make our SnapGene powered plasmid...powered by GSL Biotech's SnapGene Server Software. With the backing of SnapGene’s sequence viewer software...the features you need quickly. SnapGene’s Feature Library - SnapGene’s software seamlessly identifies ...scientists) for suggesting these changes, and the SnapGene and Addgene software development teams for working tirelessly...example below. Our old map is on the left while the SnapGene powered map is on the right (click here to see...

....  Additional resources on the Addgene blog Subscribe to the Addgene blog Read our sustainability in...requires a significant energy expenditure here at Addgene. These are critical systems that cannot be removed...only sustainability effort we’ve implemented at Addgene. The kitchen and office areas provide multiple ... the lab as well. For a more in-depth look at Addgene’s current lab-oriented environmental efforts check... about conservation in the lab Resources on Addgene.org Sign up for our quarterly newsletter Help other...resources by sharing plasmids Find plasmids at Addgene ...

...social media. #AddgeneCRISPRweek on Twitter From May 13 (today!) to May 17, the Addgene Twitter feed will...CRISPR overview and history. Tuesday will focus on generating knockouts using CRISPR and Wednesday will highlight...CRISPR. Hope you will join us this week at #AddgeneCRISPRweek. We updated the CRISPR guide! If you’re not...

...HIV gag, pol, rev, and tet genes. It can be used with 2nd or 3rd generation lentiviral vectors and an ...3rd generation lentiviral packaging plasmid from the Trono lab that contains the gag and pol genes. It...packaging plasmid from the Trono lab contains the rev gene. It’s meant to be used with pMDLg/pRRE and the envelope...doxycycline to the grown media resulting in target gene knockdown. Note: this plasmid was called pLKO-Tet-On... 15 plasmids requested from Addgene psPAX2  - psPAX2 is a 2nd generation lentiviral packaging plasmid...their own or through Addgene!) pX330-U6-Chimeric_BB-CBh-hSpCas9 - The S. pyogenes Cas9 (SpCas9) has been...plasmid predates Addgene’s founding. Find pCMV-VSV-G. pRSV-Rev - This 3rd generation lentiviral packaging...

... also modify other properties such as endogenous gene expression, which can lead to changes in normal ...neuronal firing. What is chemogenetics? Chemogenetics relies on genetically introducing engineered receptors... overview of the chemogenetics system. Why use chemogenetics Because chemogenetic receptors are activated...neuroscience Resources on addgene.org Learn more about chemogenetics in our chemogenetics guide Find viral preps...equipped using fast-acting optogenetic tools. The advantages provided by chemogenetics are instead that it is...longer-lasting effects. Building your chemogenetic system To set up a chemogenetic system, you’ll need to choose...most common target for chemogenetic manipulation are neurons where chemogenetic receptors can be used ...