We narrowed to 932 results for: Gene

...means summer is right around the corner. Here at Addgene, we’re always inspired by the new seasons, so we've...complex questions, and giving tips for consistently generating solid data. If you’re ready for the next steps...We’re very excited to be launching the official Addgene TikTok at the end of this month! We’ll be sharing...lab, along with some behind-the-scenes peaks of Addgene’s people, culture, and workplace. Rumor has it that...

... plasmid encoding rep and serotype-specific cap genes, and a plasmid encoding adenoviral helper sequences...viral vectors are tested in vitro and in vivo for gene expression and/or function. These data are sometimes...copies of the lentiviral Rev responsive element (RRE) gene and the cellular ribonuclease P/MRP 30 kDa subunit...vector genome titers by droplet digital PCR. Human Gene Therapy Methods, 25(2), Article 2. https://doi.org...Titer by a Novel Droplet Digital PCR Method. Human Gene Therapy Methods, 29(2), 96–103. https://doi.org/...Production AAV distributed by Addgene has been produced either in-house by Addgene scientists or through collaboration...either performed by Addgene or by the University of Pennsylvania Vector Core. In general, titering is performed...

...containing cassettes of the same gene multiple times.  Bioinformatics at Addgene After a run on our Illumina...Resources on the Addgene Blog A Look at Addgene’s QC Process Bioinformatics at Addgene The Power Behind...Previously, we provided a general overview of the QC process at Addgene. All plasmids go through this...technologies that Addgene uses in the QC process, when these technologies are used, and how Addgene keeps up with...Sequencing technologies used at Addgene Sanger sequencing (“First-generation sequencing”)  Sanger sequencing...assembles the raw data.  Addgene’s de novo assembly pipeline usually generates the correct plasmid sequence...revisit the assembly in a program called Geneious. Within Geneious, the raw FASTQ reads are used for either...

...plasmid to ensure our assembly result is accurate The gene/insert or any major feature highlighted by the deposited...sequenced by Addgene’s QC team. Email help@addgene.org to get started! Addgene’s QC process Addgene prioritizes...deposited sequence(s) in SnapGene. SnapGene annotates the plasmid maps based on SnapGene’s “Common Feature” library...Quality Control Now at Addgene Bioinformatics at Addgene Resources on Addgene.org Educational Resources... Figure 2. Addgene’s basic QC process. Created with BioRender.com. Once Addgene receives deposited...receiving the replacement plasmid at Addgene. QC is a team effort Addgene’s QC team is full of dedicated scientists... Resources Additional resources on the Addgene blog Addgene's Tips for Plasmid Quality Control Plasmids...

...Halloween is only 2 days away and here at Addgene we couldn’t be more excited! As a small, tight-knit...email system.  Our 2013 winners dressed up as Addgene's favorite lunch... a BonMe noodle meal, crammed...interested in finding out who wins this year’s Addgene costume contest, like us on Facebook and checkout...  addgene...

...these expected hits, we always get hits to “random” genes. Do these hits always mean the sample is contaminated...matters is the number of hits to the same sequence (gene). In a clean sample, there are typically fewer than... However, a large number of hits to one sequence/gene - we set our threshold at >100 hits - immediately...example, AAV-68544 contained 220 hits to the CHRM4 gene. When contamination is suspected, we proceed to...Viral Preparations from Viral DNA Extracts. Human Gene Therapy 31:664–678 . https://doi.org/10.1089/hum...purity. But Addgene is also conducting more rigorous quality control, including next generation sequencing... for transgene expression. Once packaged in AAV, this pre-recombined DNA could lead to transgene expression...

...-invasive systemic gene delivery in non-human primate (NHP) brains.  Systemic gene delivery capsids previously...Monomeric StayGold variants AAV.CAP-Mac for systemic gene transfer in primate brains Endothelial-specific ...Adeno-associated viral vectors for functional intravenous gene transfer throughout the non-human primate brain....plasmid here! Chen, X., et al. (2023). Functional gene delivery to and across brain vasculature of systemic...past decade, primary cells remain difficult to genetically edit due to toxicity from electroporation or ... under CC-BY license.   The Miyawaki lab generated a series of StayGold mutants and identified a ...mentioning that Kiryl Piatkevich and colleagues generated yet another monomeric StayGold variant (Piatkevich...

...her work has always been focused on gene transfer, from transgenes to plants, chloroplast to the nucleus...easy to express genes in, which makes it a good laboratory workhorse. Tips & tricks Addgene: Do you have ...The MoClo Plant Kit makes it really easy to clone genes for expression in plant cells. Sylvester and I chose... Now there are many, so if you want to make a 10-gene construct, you don’t have to worry about repeating...constructs to allow people to, for example, knock out a gene with simultaneous complementation, with a knockout...Marillonnet share with the scientific community via Addgene, the struggles of plant scientists and how they...Everybody's favorite parts for plant synthetic biology Addgene: Tell me about the Golden Gate Modular Cloning ...

...from that of the gene of interest, or expressed from the same transcript as the gene of interest, but ...visualize when and where the gene is expressed. Click here to view Addgene’s collection of empty backbones... a wide number of functions, including: tagging genes for elucidating their expression or localization...promoter of interest can be used to effectively monitor gene expression from that promoter in a given cell type...more about optogenetics at OpenOptogenetics and find optogeneic actuators and sensors at Addgene. Cell marking...and others. Review Addgene's collection of fluorescent biosensors here. Optogenetics: Scientists can use...implantation and generation of transgenic mice. Purification: GFP can be used as a general epitope tag for...

...dealing with a defective gene, Xm, the sequence of which is identical to the correct gene, Xw, except for a ...Adenine base editors (ABE) What if you have another gene Y, which has a defective allele Ym and you need ...first created a defective chloramphenicol resistance gene (CamR) by introducing a point mutation (H193Y). ...transformed it into E. coli harboring the defective CamR gene. Screening for surviving colonies and subsequent...system. We use a single polycistronic tRNA-gRNA (PTG) gene system to clone multiple protospacers which enables... slim chance of generating indels with base editors, keep in mind that the generation of indel sometimes...biology Resources on Addgene.org Find all CRISPR plasmids and resources at Addgene Find CRISPR base editors...