...Science: What the Future Holds and the Scientists Making It Happen, is an exercise in restraint. The very... revelations. The issues he discusses have been making the rounds in journal editorials, science social...likely range from mildly surprising to genuinely shocking. Mainstream media outlets have addressed CRISPR...recontextualized some areas of science that I’ve been thinking about a long time. For the scientists who like... Eric J. Perkins...

...Working with AAV vectors in the brain and peripheral nervous system presents a special challenge to scientists...answered this question and used it to publish!  Working with AAV vectors in neuroscience Let’s consider...consider my first challenge when I started working with AAV vectors. I wanted to use an AAV vector to express...label neurons transduced by each serotype. Working at Addgene has shown me this was far from an isolated...Results from a survey conducted by Addgene in 2021 asking scientists what data would improve our AAV Data...

...PMCID: PMC110254. 3. In-vivo RGB marking and multicolour single-cell tracking in the adult brain. Gomez-Nicola...pre-clinical/early clinical stages. Researchers are also working to make lentiviral vectors safer. Since lentiviral...Winemiller A, Andre K, Stein D, Blick G, Greenberg RN, Kinder C, Zolopa A, Binder-Scholl G, Tebas P, June CH,...therapy approaches to sickle cell disease. Hoban MD, Orkin SH, Bauer DE. Blood. 2016 Feb 18;127(7):839-48. ...

... a molecular leash for your protein. If you’re working with an uncharacterized protein, or a protein for...for antibody-based purification; has inherent enterokinase cleavage site GST Large Protein 26 Purification...context of a cell. FLAG tags can be cleaved by enterokinase, which recognizes DDDDK^X, cleaving after the...rounds of purification, with the cleavage reaction taking place between column runs. The TEV protease itself... Eric J. Perkins...

...Fujii: In the last several years, my lab has been working on development of technologies for biochemical ... molecules even enables us to skip the step of knocking-in. In 2013, we showed that isolation of a single...in the near future. Addgene: Has your lab been working on any other technologies that you would like to...that involvement in the scientific community is a kind of duty for researchers when considering the peer-review... in my lab then when they become independent. Looking for enChIP Plasmids? All Hodaka Fujii lab plasmids...

...that end, here are my personal 10 commandments for making it all work – most of the time. #1 Thou shalt find...happy at work, you will find balance. If you enjoy working and come home happy in the evening, your friends...of support to help with elder care, carpooling, cooking on nights when you can’t get home, babysitting ... really hard not to do this. For a family with working parent(s), the sooner your kids can make a decent...(except I am glad she never told me how much hitchhiking she did in Israel until after the fact). #9 Thou...

... used for protein localization, isolation, and tracking experiments (Rizzo, 2009). With many different...aggregation and cross-react with anti-GFP antibodies, making them difficult to use in a multicolor/multichannel...fluorophore-labeled antibodies with very specific reactivities making them more useful for multicolor imaging experiments...Davidson, and David W. Piston. "Fluorescent protein tracking and detection: fluorescent protein structure and...Blog Rabies and neuronal tracing Retrograde AAV: Making the journey from axon to nucleus AAVs CREATEd for...

...cargo Optogenetic proximity labeling and TransitID Tracking cellular history with protein ticker tape Teaching...effective and with no additional off-target edits, making them ideal for size-limited AAV delivery. PE6c ..., et al. (2023). Dynamic mapping of proteome trafficking within and between living cells by TransitID.... bioRxiv – doi: 10.1101/2023.02.07.527548.    Tracking cellular history with protein ticker tape By Brian...call for thin sections of tissue, the Wu Lab is working to create a three-dimensional atlas of protein ...

...amazing 111 countries (Figure 1)! But while we were making the materials available to many countries, import...stays in transit for a certain number of days and tracking average transit time for each courier we contracted... to increase accessibility to our repository by making it easier to find materials and learn about them...information are truly accessible, not just available.   Looking forward Over the past twenty years, Addgene has... accessible to scientists across the globe?” By asking this question, and by listening to the answers ...

...make sure your plasmid has a promoter suited to working in your host organism. Because transcription machinery... and there is very little crossover. Generally speaking, promoters in bacteria are less diverse and complex...elements, insulators, and silencers; however, some "leakiness" of transcription may occur. This is normally ...expression mRNA Mammalian promoter from phosphoglycerate kinase gene.  Constitutive  Widespread expression, but...optogenetics mRNA  Ca2+/calmodulin-dependent protein kinase II promoter Specific Used for neuronal/CNS expression...