We narrowed to 605 results for: RAN-1

...functioning T cell repertoire (1). In this example, it was possible to transduce the T-cells outside the body...significant improvements in vision over a period of 1 to 3 years – the treatment appears to be both safe...research and science communication.     References 1. Gaspar, H. B., S. Howe, and A. J. Thrasher. "Gene...their mutations corrected, and the T-cells were transferred back into the infants. Almost all patients have...techniques may hold the key. In the simplest form, translating gene therapies from conceptual design to clinical...that carries a small, approximately 4.7 kb single stranded genome. AAV belongs to the family Parvoviridae...AAV vectors are also capable of infecting a wide range of cell types including but not limited to muscle...

..., check out Addgene's CRISPR Guide. Figure 1: Like this jar of jellybeans, a pooled CRISPR library... selecting a CRISPR library for your experiments. 1.  What species are your cells derived from? Currently...a different gene. Libraries may be available in a 1-plasmid system, in which Cas9 is included on the gRNA-containing... non-specific endonuclease (Cas9) and a single stranded guide RNA (gRNA). The ~20 nucleotide targeting...of wild-type Cas9 and a gRNA generates a double-strand break in the target DNA, which, when repaired through...scaled up for genome-wide screening due to the broad range of potential target sequences and ease of generating...heterogeneous population of gRNA-containing lentiviral transfer vectors, each targeting a specific gene within...

...Bacillus. Appl Microbiol Biotechnol. 2019 Jan;103(1):303-313. doi: 10.1007/s00253-018-9485-4. Epub 2018...and clonal analysis in Drosophila.” Nat Methods 9(1) (2011): 47-55. PubMed PMID: 22205518. PubMed Central...Methods Mol Biol. 2016;1478:53-78. doi: 10.1007/978-1-4939-6371-3_3. PMID: 27730575; PMCID: PMC5270762. ... and lac promoters.” Proc Natl Acad Sci U S A. 80(1) (1983): 21-5. PubMed PMID: 6337371. PubMed Central... polymerase and transcription factors. Since the promoter region drives transcription of a target gene...repressor then prevents transcription from occurring, which means that transcription is now OFF.   We’ll...tetracycline-controlled transactivator (tTA). They fused TetR with the transcriptional activation domain VP16...

...label surface proteins with a membrane-impermeable biotin substrate (Figure 1). Biotinylated proteins were...adhesion molecules, transporters, ion channels, GPCRs, and more.  Figure 1: Workflow for cell-...564–571. https://doi.org/10.1038/s41586-022-05682-1 Zhang, Y., et al. (2025). Probing condensate microenvironments... and synthetic biology. Nature Communications, 15(1), 6563. https://doi.org/10.1038/s41467-024-50767-2...required, through mitochondrial transfer. In the two decades since this transfer was first proposed, researchers...manipulation transplantation (AMT), CRISPR-modified adipocytes (fat cells) are transplanted as a mass that...visible chromoproteins in a cell-free transcription-translation system (Figure 6). This approach eliminates...

... when (1) other strategies may not be feasible and (2) large-scale proteomic and transcriptomic datasets...expected in another. You’ll need to validate that points 1 and 2 hold true under your specific experimental conditions...application and available resources.     Figure 1: Table summarizing the IWGAV five proposed antibody...validation: A view from the mountains. New Biotechnol 45:1–8. https://doi.org/10.1016/j.nbt.2018.08.002  Uhlen...Interest (GOI), and transfect it into your favorite cell line. In parallel, you transfect cells with another...antigen - Recognition of an immunogen does not guarantee recognition of the target antigen. You want to...Antibody-independent methods may include analysis of transcriptomic or proteomic data from several different tissues...

...Zhang et al., 2015). In this system a modified TIR-1 protein can degrade degron-tagged proteins in an auxin...splitting cGAL4 in two and binding each half to a gp41-1-N-intein. Split inteins are protein domains that naturally...mutations with unc- 119 + selection." MicroPublication 1 (2018): 2–3. Wang, Han, et al. "cGAL, a temperature-robust...neurons from each class to fully characterize the transcriptome. The first data release for CeNGEN is planned... The 3’UTRs of mRNA are important for post-transcriptional regulation in C. elegans. To study the role...using a split intein for refined spatiotemporal transgene control in Caenorhabditis elegans." Proceedings...

...and P. mirabilis, had only ~1 transfer per 1,000,000 colonies. Higher transfer rates are better suited for...non-selective plate. Most bacteria tested had 1 transfer in every ~100 to 1,000 colonies, but some bacteria...active for greater than 50 generations. Figure 1: Mobile-CRISPRi's modular components. Image from Peters...protocols can transfer CRISPRi into a single bacterial strain, such as a B. subtilis, or a narrow range of bacterial...can be introduced into a broader range of bacteria. Once transferred, Mobile-CRISPRi stably integrates...bacterial Tn7 transposon system. This system has two parts: a plasmid expressing the Tn7 transposition genes ...chromosomal copy of the RP4 transfer machinery mobilize these plasmids for transfer to recipient cells during...

... (which range between 0 and 100) are then converted to a weight (which ranges between 0 and 1) so that...values (ranging from -40 bp to 40 bp) are also converted to a weight (which ranges between 0 and 1) (shown...distance) This gives each guide a score ranging between 0 and 1. The cutting frequency determination (CFD...summary per batch. Ranking guides for HDR in-depth In other tools, guides are ranked by specificity of ...genome by eye. We came up with a ranking formula that does just this: Rank = (compressed CFD score) * (gaussian...interactions (e.g., scores >80) are transformed to 100. This transformation (illustrated in the graph below...employs biological best practices. For example, it ranks guides by CFD score, and it mutates HDR donor templates...

...activating kinase 1 (MEK1) with either one or two phosphoserine residues cotranslationally inserted in their...directionally assembled into a Level 1 vector creating a single transcriptional unit (Ex: a promoter, 5’UTR, ...expression. Brown et al., Genesis 2014 Jan;52(1):39-48. APEX2 for proteomic mapping and electron...engineering cells that lack release factor one (RF-1; Bacterial strain EcAR7) and minimizing the set of...comprised of three sets of cloning vectors (Level 0, 1, or 2) which can be utilized in three successive assembly...coding region, and terminator). Next, up to six Level 1 modules can be similarly assembled into a Level 2 ...vectors to allow for additional iterations of Level 1 assembly if necessary. Combining multiple Level 2 ...

...Please see Figure 1 for commonly used markers for major mammalian organelles and transport pathways. It is...cell with the images from the Allen Cell Explorer (1). When studying the function of a protein or its role...Subcellular Localization Poster here! References 1. Allen Institute Plasmid Pages 2. Cooper GM. 2000....can be gained by understanding the location and transport of the protein within a living cell. Analyzing...Analyzing differences in protein localization and transport between healthy and diseased states can also provide...endoplasmid reticulum, modified in the golgi and then transported in vesicles via the secretory pathway to their...the end of their life cycle, proteins may be transported through the vesicles to the lysosome where they...