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We narrowed to 644 results for: Uty

Showing: 251 - 260 of 644 results
  1. Truncated gRNAs for Regulating Gene Expression

    Type
    Blog Post
    Published
    Jan. 10, 2017, 3:37 p.m.
    ...Inspired Engineering. CRISPR/Cas9 technology has revolutionized the fields of molecular biology and bioengineering...Traditional Cas9 regulators In addition to its utility in making targeted modifications to DNA, Cas9 can...Furthermore, among those Cas9 orthologs that have been scrutinized, the majority have been shown to exhibit more...
  2. PhD Applications After COVID

    Type
    Blog Post
    Published
    Jan. 5, 2023, 2:15 p.m.
    ...department during the application process. Many institutions recommend that you reach out to potential advisors...you can’t visit. By talking with people at the institution, you can get a better sense of the environment...answer process can also help you to see if the institution is right for you outside of just the program ...
  3. Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells

    Type
    Blog Post
    Published
    Dec. 10, 2020, 1 p.m.
    ...the use of AdV for in vivo CRISPR-Cas delivery utilized the Non Homologous End Joining (NHEJ) DNA repair..., 2014). An alternative approach to NHEJ is to utilize the Homology Directed Repair (HDR) to knock in ...and very efficient introduction of DNA into therapeutically relevant, non-transformed mammalian cells. ...
  4. Multiplexed Capture of Promoter-enhancer 3D Chromatin Structures Using CRISPR

    Type
    Blog Post
    Published
    July 2, 2020, 12:40 p.m.
    ...hard to make out the details. To increase the resolution, the Xu lab created a dCas9-based CAPTURE (CRISPR...enhancers. CAPTURE 2.0’s multiplexing and high resolution allowed the Xu Lab to simultaneously characterize...chromatin's dynamic role in gene regulation The high resolution and multiplexed chromatin ‘photos’ generated ...
  5. Genome-wide Screening Using CRISPR

    Type
    Blog Post
    Published
    Aug. 18, 2015, 2:30 p.m.
    ...are treated with a drug of interest and gRNA distribution is analyzed in the drug-resistant population... resistance to drugs and can identify future therapeutic targets for diseases causing uncontrolled cell...6 gRNAs per target gene, and maintaining the distribution of each gRNA within the population is key. Loss...
  6. Transferable Skills Guide: Identifying Your Transferable Skills

    Type
    Blog Post
    Published
    Jan. 11, 2018, 3:29 p.m.
    ...their problem and piecing together a really great solution that covered all the bases. You may have also ...skills to convey your empathy and your awesome solution to the person over email. Examine your current...Speaking Time management Managing a team Conflict resolution Organization Leadership Problem Solving Creativity...
  7. Antibodies 101: Producing Recombinant Antibodies

    Type
    Blog Post
    Published
    Aug. 13, 2024, 1:15 p.m.
    ...unlimited production, reliable expression, and easy distribution as DNA (Trimmer, 2020), you may be interested...The beads are then washed, followed by antibody elution. Whatever method you choose, you may not be able...any temperature shifts. Of course, we are also cautious during the aliquoting and storage process to prevent...
  8. AAVs CREATed for Gene Delivery to the CNS and PNS

    Type
    Blog Post
    Published
    Sept. 28, 2017, 2:01 p.m.
    ... challenge. Find the PHP Plasmids Here! Their Solution: Use the CREATE method to engineer two new IV ...stands for Cre Recombinase-based AAV Targeted Evolution. In this technique, a pool of mutant AAV capsid...labeling is to inject a mix of AAV vectors that constitutively express a red, green, or blue (RGB) fluorescent...
  9. CRISPR 101: RNA Editing with Cas13

    Type
    Blog Post
    Published
    July 31, 2020, 12:30 p.m.
    ...They then performed sixteen rounds of directed evolution across the full ADAR2DD to further optimize cytidine...   Addressing the potential use of RESCUE in therapeutic applications, the authors tested various dRanCas13b...of RNA editing in research and its potential therapeutic uses We are also curious if REPAIR and RESCUE...
  10. Introducing Addgene's New Search - Find Plasmids More Easily For Your Research!

    Type
    Blog Post
    Published
    Dec. 3, 2019, 11:38 a.m.
    ... GCaMP containing plasmids are available for distribution. If your experiment involves delivering a calcium...types of searches including depositing labs and institutions. For example, search for “Melina Fan” and you...Lab published in eLife. Addgene is currently distributing 94 plasmids expressing validated antibodies ...
Showing: 251 - 260 of 644 results