We narrowed to 432 results for: sequence

...host machinery to make additional copies. ori sequences are generally high in As and Ts. Why, you ask?...that affect plasmid copy number  Although the sequence and regulation of the ori dramatically affect ...

...often used to quantify DNA for next generation sequencing. Unlike absorbance-based methods, fluorescence-based...method is often used preceding next-generation sequencing or microarray studies and is not used as much...

...vectors and plasmids with repeating sequences. Repeating sequences within a plasmid can undergo intramolecular...

.... For example, Dave Grawoig is using nanopore sequencing at BosLab to perform proof-of-concept experiments... to collect various soil samples, extract DNA, sequence it, and analyze the data to characterize the microbiomes...

...to target an ssRNA of interest, for example, a sequence specific to a virus or pathogen. Once Cas13 recognizes...recognizes and binds to the programmed sequence, it can promiscuously cleave surrounding ssRNA molecules...SHERLOCK. Cas12a is targeted to a specific DNA sequence, such as the Human papilloma virus (HPV) genome...

...amino acid sequence required for BirA recognition and biotinylation. This 13 amino acid sequence is termed...Biotin can be added to either terminus of a DNA sequence during a Polymerase Chain Reaction (PCR) by using...

... Both the plasmid (blue, backbone) and the DNA sequence of interest (green, insert) are cut with restriction...in the direction you want - usually by Sanger sequencing or further restriction digests. Of course there...appropriately sized insert, you should send it for sanger sequencing using primers that will allow you to read over...

... of DNA to create a synthetically designed DNA sequence. There are multiple methods of DNA assembly available...experimental and primer design based on fragment sequences and the polymerase used in the amplification. ...

...eukaryote (S. cerevisiae) to have its genome fully sequenced There are several human disease models established...Genome manipulation K. phaffii’s genome has been sequenced for some time, clearing the greatest hurdle for...

... downstream methods, such as qPCR or next-gen sequencing. ChIP is an incredibly useful application for...suitable for isolated and purified DNA, such as sequencing or qPCR. Yay!  Where’s the challenge in that?...