We narrowed to 897 results for: ANT

...water of HR work. What do you think is the most important resource that Addgene provides the scientific ... how to operate during the pandemic, but it’s important to develop a plan for moving forward. Sooner or...

...accomplishments. Employers and academic departments want to be able to tell that you know about something...Learned Sooner by Peggy Klaus Looking for a mentor or want to start a peer mentoring group? "What Makes a ...

...choosing a specific serotype, but occasionally you may want to further control where your gene of interest is... many more tips and ideas on how to use these fantastic tools in your experiments.  Many thanks to Hongkui...

... they’ve engineered SECURE adenine base editor variants using a protein-truncation strategy with reduced...Transient expression of CRISPR/Cas9 in cells has advantages like reduced off-target activity and decreased...

...and see if we should start celebrating papers and grants too with pizza or pub.” The Cepko Lab celebrates...required to achieve success in the lab, it is important to take the occasional breather and acknowledge...

...Organize you experiments, keep track of upcoming grant deadlines, and sync everything to your calendar.... updates to protocols in real time. You may also want to consider moving your paper spreadsheets for reagent...

...double strand breaks. Researchers have long taken advantage of this natural process to integrate protein tags...selectable gene product for isolation of successful transformants. Addgene distributes several ready-to-use, modular...

...physical scientists are underrepresented. It’s important to note that these results could be biased for...Resources Check out the Top 50 Science Stars on Twitter Want suggestions on how to use social media as a scientist...

...increasing transgene cargo capacity, enhancing anterograde transport efficiency, and engineering viral capsids...development of this practical guide by the workshop participants  - a testament to openness and sharing in science...

... for you to find the plasmids and publications relevant to your research interests. We do this by promoting...other resources. If you think we’re missing an important collection or if you think a particular plasmid...

...loss-of-function mutants could provide a streamlined, one-size-fits-most approach. evoCAST is an important step toward...

...Scholars summer program. I was unsure whether or not I wanted to major in biochemistry. There, I had exchanged...has changed my life for the better, and I wouldn’t want it any other way. Toj Toj-Villagran is a rising ...

...reagents.  mAb3D atlas validated antibodies: A database of validated antibodies for mouse, human, and marmoset...

...researchers to select and generate physiologically relevant models. In complex diseases, it can aid in generation... line knock-ins of patient mutations which are variants of unknown significance – mutations with unclear...

... Society, or maybe you think about a local food pantry or community arts organization. Many people are...resources continuously raising money from donations or grants to fulfill their mission. Addgene is fortunate ...

...Experiments Experiment  Detection Method Notes Quantification of a process Plate-based assays (plate reader... HPLC (proteins or other molecules) Isolate or quantify cells, proteins or other molecules that have been...

...worthless. But simply writing down your data doesn’t guarantee a publication. There is an art to composing manuscripts.... Practice writing everyday Overall, the most important instruction the classes taught me was to write...

...effector gene. Effector genes, any gene that you want to control the expression of using the SPARC system...length of the truncated attP site. The three SPARC variants, based on the length of the attP site, allow for...

... et al calls this method acoustic collapse.  Importantly, acoustic collapse doesn’t affect bacterial viability... of ARG constructs with new acoustic phenotypes. Want to start using acoustic reporters for your research...

...different cellular responses. One of the biggest advantages of such a tool is being able to identify specific...screening technique. Lastly, the activity of both antagonists and inverse-agonists could be measured using ...

...two inverted terminal repeats (ITRs); but when recombinant AAV (rAAV) is made in the lab, rep and cap are...2020) Adeno-Associated Virus Genome Interactions Important for Vector Production and Transduction. Human ...

...This series was written for selfish reasons: I wanted to learn about careers in science communication...summarizing mechanisms and applications that are relevant to things IDT does. I also work on our website...

...chemistry degrees and provide advice to students who want to study chemistry. The society currently has 352...engaging with young scholars. We believe it’s very important to develop a network of fellow scientists that...

...simply put, I moved into this position because I want to help make the research enterprise work better...at Harvard, I recognized that I would ultimately want to go into science communication but thought that...

... and data with authors and referees. And I am constantly teaching – often explaining a technical piece...focuses at Science on developmental neurobiology and plant sciences, viewed through the lens of development...

...as a drug-screening platform to identify new antifungals that target calcineurin. Current calcineurin ...consisting of a quickly-degraded destabilized GFP variant fused to a 4X CDRE repeat. In addition to showing...

...through manuscripts, seminars, or posters. It is important to note that it is the actual communication itself...quickly!  The question   The first, and most important question, you should ask for any science communication...

...that produce useful natural materials, such as antibiotics. Simon Moore and Paul Freemont’s lab previously...mCherry and GFP expression to check for ABE’s. b) Quantification of base-editing efficiency using XMAS-TREE-...

... plasmids now packaged in AAVrg. jGCaMP7s, the variant with the most sensitive response to action potentials...to measure glutamate levels. iGluSnFR.A184S, a variant with higher affinity to glutamate, has been packaged...

...were known to be diversity hotspots containing abundant biological dark matter and so the lab organized...application from the publication is the removal of unwanted cDNA derived from 16S rRNA in bacterial RNA-Seq...