We narrowed to 798 results for: KIN

...of conjugation, and these are encoded by certain kinds of plasmids — more on that in a minute! Fun fact...

...tips and tricks for overcoming these hurdles. Talking 'bout my (lentiviral) generation The first step...

...process. Figure 1: Begin the deposit process by clicking “Submit Online Deposit”or “Download Deposit Spreadsheet...

...pipelines (viral), and researchers are currently working on giving this treatment a CRISPR spin. CRISPR ...

...References Lu, Q. L., & Partridge, T. A. (1998). A New Blocking Method for Application of Murine Monoclonal Antibody...

...HY, Tortorici MA, Veesler D, Murphy M, Pettie D, King NP, Balazs AB, Bloom JD (2020) Protocol and Reagents...

...associate, self-excise, and covalently ligate their flanking peptides together (Wang et al., 2018).” With the...

...Nanoblades? They’re easily produced in a BSL2 lab, making them a versatile and accessible genome editing ...

...mean it’s suitable for your experiment. If you’re looking for more pan-genome PAM flexibility, you’ll want...

...eBook to get all the background you need to start working with plasmids! What are termination and polyadenylation...

...antibodies and pharmaceuticals, specifically interleukins. Yeast expression systems Yeast are a great ...

...membrane-bound spike protein, in a remarkable 12 working days. While the lab used this method for COVID-...

...relative quantification - that is, since you’re looking at the amount of DNA in your sample relative to...

...gene in the recipient's genome. Antibiotic plates lacking DAP select against donor bacteria and for recipients...

...round of evolution (AID-2) to reduce some of the leakiness associated with the system, but the general concept...

...were the only realistic options for scientists working in regenerative medicine, but the longing for a...

... experiments. Considerable effort has gone into making FPs monomeric, yet many investigators remain ignorant...

...found yourself bamboozled by all of the different kinds of information on our plasmid pages? Well, to help...

...complex trendlines. If you don’t have access to this kind of software, don’t give up! Rather than plotting...

... Addgene Blog Read this blog post about CRISPR Kinome Libraries available at Addgene Learn about the ...

...Multi-color labeling of neurons is for more than making pretty pictures (see figure 4). It also enables...

...resources References Bauzon, M., Castro, D., Karr, M., Hawkins, L. K., & Hermiston, T. W. (2003). Multigene expression...

...array. Workflow and technical considerations Making insertions in bacterial genomes with the V. cholera...

...phenotypic analysis it is appropriate to begin working in the T2 generation. The selection process itself...

...been limited, in part, due to the necessity for working with this virus under the highest biosafety level...

... version of TnpB is just 408 amino acids long — making it one of the most compact genome editors to date...

...choose the best method based on the plasmid. By working with the Data team, the QC team has also improved...

...assembly of the split luciferase is reversible, making PILATeS suitable for live imaging with a time resolution...

...So, as long as it doesn't look done, just keep working on it. Also bear in mind that this is just a review...

...Cas13 enzymes require a single base protospacer flanking sequence (PFS) adjacent to the target, but many...