We narrowed to 786 results for: KIN

1. Open Resources and Plasmid Tools For Studying C. elegans

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   Blog Post

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   July 18, 2019, 12:55 p.m.

   ...associate, self-excise, and covalently ligate their flanking peptides together (Wang et al., 2018).” With the...

2. Plasmids 101: Terminators and PolyA signals

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   Blog Post

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   March 31, 2016, 2:30 p.m.

   ...eBook to get all the background you need to start working with plasmids! What are termination and polyadenylation...

3. Plasmids 101: Degron Tags

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   Blog Post

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   March 14, 2024, 1:15 p.m.

   ...round of evolution (AID-2) to reduce some of the leakiness associated with the system, but the general concept...

4. No Llamas Required - Synthetic Nanobodies Against Membrane Proteins

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   Blog Post

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   June 18, 2020, 1:15 p.m.

   ...membrane-bound spike protein, in a remarkable 12 working days. While the lab used this method for COVID-...

5. Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria

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   Blog Post

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   April 4, 2019, 12:53 p.m.

   ...gene in the recipient's genome. Antibiotic plates lacking DAP select against donor bacteria and for recipients...

6. Starter guide to induced pluripotent stem cells (iPSCs) part 1:  A renaissance in regenerative medicine

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   Blog Post

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   Oct. 30, 2018, 1:04 p.m.

   ...were the only realistic options for scientists working in regenerative medicine, but the longing for a...

7. Plasmids 101: Protein Expression

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   Blog Post

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   June 7, 2018, 1:17 p.m.

   ...antibodies and pharmaceuticals, specifically interleukins. Yeast expression systems Yeast are a great ...

8. PCR Applications for Copy Number Variation Assays

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   Blog Post

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   April 26, 2022, 1:15 p.m.

   ...relative quantification - that is, since you’re looking at the amount of DNA in your sample relative to...

9. When is a Monomer not a Monomer? The Top Three Ways Your Favorite Fluorescent Protein Oligomerizes in Cells

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   Blog Post

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   April 19, 2016, 2:30 p.m.

   ... experiments. Considerable effort has gone into making FPs monomeric, yet many investigators remain ignorant...

10. Anatomy of a Plasmid Page at Addgene

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    Blog Post

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    Feb. 4, 2016, 3:30 p.m.

    ...found yourself bamboozled by all of the different kinds of information on our plasmid pages? Well, to help...

11. Great Results Start with Great Standard Curves

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    Blog Post

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    Aug. 15, 2024, 1:15 p.m.

    ...complex trendlines. If you don’t have access to this kind of software, don’t give up! Rather than plotting...

12. An Introduction to Adenovirus

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    Blog Post

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    Oct. 3, 2023, 1:15 p.m.

    ...resources References Bauzon, M., Castro, D., Karr, M., Hawkins, L. K., & Hermiston, T. W. (2003). Multigene expression...

13. Tips for arabidopsis transformation

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    Blog Post

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    Oct. 25, 2018, 1:23 p.m.

    ...phenotypic analysis it is appropriate to begin working in the T2 generation. The selection process itself...

14. New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries

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    Blog Post

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    Oct. 4, 2018, 12:44 p.m.

    ... Addgene Blog Read this blog post about CRISPR Kinome Libraries available at Addgene Learn about the ...

15. INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

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    Blog Post

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    Dec. 7, 2020, 2:53 p.m.

    ...array. Workflow and technical considerations Making insertions in bacterial genomes with the V. cholera...

16. AAVs CREATed for Gene Delivery to the CNS and PNS

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    Blog Post

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    Sept. 28, 2017, 2:01 p.m.

    ...Multi-color labeling of neurons is for more than making pretty pictures (see figure 4). It also enables...

17. Minigenomes - a Safe Way to Study Dangerous Viruses Like the Ebola Virus

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    Blog Post

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    March 17, 2016, 2:30 p.m.

    ...been limited, in part, due to the necessity for working with this virus under the highest biosafety level...

18. Hot Plasmids: Fall 2024

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    Blog Post

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    Nov. 21, 2024, 1:30 p.m.

    ...assembly of the split luciferase is reversible, making PILATeS suitable for live imaging with a time resolution...

19. How to Write a Scientific Review Article

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    Blog Post

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    Feb. 16, 2017, 3:30 p.m.

    ...So, as long as it doesn't look done, just keep working on it. Also bear in mind that this is just a review...

20. The PAM Requirement and Expanding CRISPR Beyond SpCas9

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    Blog Post

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    Aug. 20, 2020, 2:30 p.m.

    ...Cas13 enzymes require a single base protospacer flanking sequence (PFS) adjacent to the target, but many...

21. Four Base Editing Reporters to Monitor and Enrich Editing in Real-time

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    Blog Post

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    July 7, 2020, 1:15 p.m.

    ...APOBEC-Cas9 editosome would restore eGFP fluorescence, making it easy to quantify single base editing efficiency...

22. CRISPR-mediated Plant Base Editors

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    Blog Post

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    Jan. 3, 2019, 1:35 p.m.

    ... repaired by cellular mismatch repair machinery making an original C-G pair converted to T-A or reverted...

23. Bacterial Toxin-antitoxin Systems as Molecular Biology Tools

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    Blog Post

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    Nov. 1, 2018, 12:35 p.m.

    ...through a second recombination event between the two flanking repeats. In cells where recombination has not ...

24. New Tools Enable CRISPRa for Neuroscience Applications

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    Blog Post

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    July 2, 2019, 12:51 p.m.

    ...neuron-specific promoters, including calmodulin-dependent kinase II alpha (aCaMKII; excitatory neurons) and Synapsin...

25. Plasmids 101: Control Plasmids

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    Blog Post

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    April 9, 2015, 3:29 p.m.

    ...results can be compared; results from an experiment lacking the proper controls are invariably inconclusive...

26. CRISPR 101: Homology Directed Repair

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    Blog Post

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    Jan. 26, 2023, 2:15 p.m.

    .... Li, K et al. PLoS ONE 9(8); (2014). PubMed. Making designer mutants in model organisms. Peng, Y. et...

27. CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

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    Blog Post

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    Oct. 11, 2016, 2:30 p.m.

    ... you chose for your experiment, but, if you’re working with plants, don’t be afraid to try your hand at...

28. Quick Guide to Near-Infrared Fluorescent Proteins

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    Blog Post

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    Oct. 5, 2021, 1:15 p.m.

    ...lamps are better, and xenon lamps are the best for working in this spectral range. You can also check that...

29. Fluorescent Proteins 101: When GFP lets you down

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    Blog Post

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    Aug. 23, 2018, 12:05 p.m.

    ...Chartrand. "Using fluorescent proteins to study mRNA trafficking in living cells." Methods in cell biology 85 ...

30. Treating Muscular Dystrophy with CRISPR Gene Editing

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    Blog Post

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    Jan. 26, 2016, 3:30 p.m.

    ...low to no off-target activity at the ten highest-ranking predicted off-target sites. Each paper characterizes...