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  2. In Vivo Biotinylation of Bacterial Fusion Proteins

    Type
    Blog Post
    Published
    Jan. 25, 2018, 2:09 p.m.
    ...through biotin-avidin binding could provide a true measurement of high affinity (i.e. sub-nanomolar) protein-ligand...map and cartoon below). Including biotin in your culture and inducing protein expression with IPTG results...

  3. Using Addgene's New Viral Service for Your Research

    Type
    Blog Post
    Published
    Nov. 17, 2016, 3:30 p.m.
    ...questions help us expand and improve our resources for future users. We’re excited to be distributing new types...fulfilled more than 140 orders for virus! We’ll be sure to keep you updated on our progress and would love...

  5. Celebrate Open Data Day with Addgene’s AAV Data Hub!

    Type
    Blog Post
    Published
    March 5, 2020, 2:15 p.m.
    ... tool. They can search the database by certain features like serotype, promoter, or tool type and each...use these questions as a guide for designing your future injection experiments. Have your data or lab notebook...

  6. New and Upcoming Viral Vectors - December 2019

    Type
    Blog Post
    Published
    Dec. 19, 2019, 2:40 p.m.
    ...spatiotemporal resolution required for precise in vivo measurements of dopamine (113049, 113050) or norepinephrine...Looger’s lab at Janelia for neuroscientists to measure glutamate levels. iGluSnFR.A184S, a variant with...

  7. Bright Monomeric Fluorescent Proteins: mNeonGreen, mTFP1, and mWasabi

    Type
    Blog Post
    Published
    April 25, 2019, 3:01 p.m.
    ...lanceolatum in 2012. Using structural modeling and structure-guided direct evolution, Nathan Shaner and colleagues...protein derived from Branchiostoma lanceolatum." Nature methods 10.5 (2013): 407. PubMed PMID: 23524392...

  8. Hot Plasmids and Viral Preps - September 2021

    Type
    Blog Post
    Published
    Sept. 21, 2021, 1:15 p.m.
    ... been difficult to modify in the past.   Figure 1: Identification and enrichment use of XMAS-TREE...splice acceptor and donor sites, and introducing premature stop codons.    New from the viral service By...

  9. Plasmids 101: A Brief History of Plasmids and an Improved eBook!

    Type
    Blog Post
    Published
    Oct. 29, 2015, 2:30 p.m.
    ... "Viral Expression Vectors" chapters, and more pictures and pro tips.  Happy reading! Learn more about...PMCID: PMC427208. Novick, R P et al. “Uniform Nomenclature for Bacterial Plasmids: A Proposal.”Bacteriological...

  10. Rewiring Metabolic Circuitry with CRISPR RNA Scaffolds [Video]

    Type
    Blog Post
    Published
    April 7, 2015, 4:21 p.m.
    ...interspaced with linkers that can fold into a secondary structure that can bind specific proteins via aptamer domains...using RNA scaffolds to assemble proteins in vivo. Nature protocols. 7(10) 1797-1807 (2012). PubMed. ...

  12. Learning Technical Skills at a Lab Central Workshop

    Type
    Blog Post
    Published
    Feb. 20, 2018, 2:30 p.m.
    ... Julie from Novartis who was working with cell culture and who was extremely helpful since she was familiar...useful pieces of information from the workshop. Future workshops for Addgene Addgene strives to be a great...

  13. Pushing the Limits of DNA Assembly

    Type
    Blog Post
    Published
    Sept. 6, 2017, 1:01 p.m.
    ...? Check Out Our Plasmids 101 eBook! One of the features that makes NEBuilder HiFi so accurate (apart from...incorporation of a 3’-5’ exonuclease in the reaction mixture. The exonuclease removes mismatched 3’ overhangs...

  14. Viral Vectors 101: Viral Vector Elements

    Type
    Blog Post
    Published
    July 25, 2023, 1 p.m.
    ...with each other. Below we will outline the common features as well as the differences within their viral ...Kulanayake, S., Tikoo, S. K. Adenovirus Core Proteins: Structure and Function. Viruses. 2021 13(3):388. DOI:10.3390...

  15. Viral Vectors 101: Viral Applications

    Type
    Blog Post
    Published
    April 13, 2023, 1:15 p.m.
    ...variable being manipulated. Thus, the adjustable nature of viral libraries makes them the ideal vector ...low immunogenicity, and cell-specific targeting features make them an ideal delivery system for human patients...

  16. Top Requested Lentivirus and AAV of 2016

    Type
    Blog Post
    Published
    Jan. 6, 2017, 3:56 p.m.
    ...lentiCas9-blast to generate Cas9-expressing cells. To ensure that this growth rate effect doesn’t confound your... that express cre recombinase. Typically, this feature is used to attain cell-specific expression in transgenic...

  17. GPCRs: How Do They Work and How Do We Study Them?

    Type
    Blog Post
    Published
    Feb. 11, 2021, 2:15 p.m.
    ...modified Tango beta-arrestin recruitment assay to measure receptor activation for more than 300 GPCRs. In...peroxidase-catalyzed proximity labeling to quantifiably measure GPCR agonist response in vivo (Paek et al., 2017...

  18. Behind the scenes: Addgene’s new search engine and more

    Type
    Blog Post
    Published
    Dec. 12, 2019, 2:22 p.m.
    ...synapsin-1, human synapsin, hSynap. Matching We capture multiple variations of the same information in ...resources from the Addgene blog Learn about other features on Addgene's website Find a step-by-step guide...

  20. Will You Be My Mentor? Finding and Asking for Mentoring Support

    Type
    Blog Post
    Published
    Feb. 4, 2014, 3:50 p.m.
    ...information so you can tap them for advice. As a future alum, these are people you can “cold call” and ...time. Consider this option but be cautious and make sure to choose one that comes highly recommended from...

  21. Plasmids 101: The protein expression toolbox

    Type
    Blog Post
    Published
    Feb. 16, 2023, 7:05 p.m.
    ...integrated (once or many times) into the genome. Make sure to choose an appropriate promoter to express your...immediate and target-specific protein degradation. Nature Chemical Biology. May 2018. 14(5): 431-441. PubMed...

  25. Rabies and Neuronal Tracing

    Type
    Blog Post
    Published
    May 29, 2018, 1:51 p.m.
    ... of us learned in biology is that the body’s architecture and plumbing are important. We started with ...oxygenates the body. However, in neuroscience, this architecture is frequently still an open question. The connections...

  26. Hot Plasmids and Viral Preps - January 2021

    Type
    Blog Post
    Published
    Jan. 5, 2021, 2:15 p.m.
    ...spatiotemporal phenotypes from large heterogeneous cultures.  HeLa cells expressed a fusion of keratin...GFAP.SF-iGluSnFR.A184S-AAV1 here New Dual and Triple feature INTRSECT viral preps from the Deisseroth Lab now...

  28. Experimenting in an Open Source lab: from CRISPR to Cats

    Type
    Blog Post
    Published
    July 23, 2015, 2:30 p.m.
    ..., in the form of workshops, presentations, and lectures. There are currently around 1,000 active makerspaces...discarded. For instance, the centrifuge in the picture had a broken controller and would not spin. It ...

  29. Plasmids 101: Degron Tags

    Type
    Blog Post
    Published
    March 14, 2024, 1:15 p.m.
    ...here, the SMASh tag does offer a few other unique features. If your protein is not amenable to being tagged...effective it will be, given the diversity of protein structures, interacting partners, and localization. Thankfully...

  30. PhD Applications After COVID

    Type
    Blog Post
    Published
    Jan. 5, 2023, 2:15 p.m.
    ...presentation. It can help soothe your nerves and make sure you cover everything you need to during the interview...students; an online meeting can take some of that pressure off. This question and answer process can also...
Showing: 751 - 780 of 870 results