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Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 705 results for: ANG

Showing: 501 - 520 of 705 results

  1. New Tools Enable CRISPRa for Neuroscience Applications

    Type
    Blog Post
    Published
    July 2, 2019, 12:51 p.m.
    ...AAV). This issue was recently circumvented by the Yang Lab with the development of a Suntag-p65-HSF1 (SPH...as well as individual neuronal populations.  The Yang Lab’s AAV plasmids used to express Cre under neuron-specific...

  3. Data Freedom: The Expansion of Data Sharing in Research Publications

    Type
    Blog Post
    Published
    Aug. 5, 2014, 6:51 p.m.
    ...supporting datasets must be logically annotated and arranged, as in an actual publication and not require interaction...take interest in how it is released, we will be in danger of having to deliver on well-meaning but impractical...

  4. Quick Guide to Near-Infrared Fluorescent Proteins

    Type
    Blog Post
    Published
    Oct. 5, 2021, 1:15 p.m.
    ...lamps are the best for working in this spectral range. You can also check that no filters and/or cold ...tell us about cellular events via  fluorescence changes occurring due to protein synthesis, degradation...

  5. Tips for Getting a Faculty Position

    Type
    Blog Post
    Published
    July 20, 2023, 1:15 p.m.
    ...the nuances of the research in as technical of language as possible. Seriously. My suggested litmus test...knowledge that you have given everyone a simple plain language map of where you are going and what you expect...

  6. Plasmids 101: Expressing shRNAs for RNA Interference

    Type
    Blog Post
    Published
    June 17, 2025, 1:15 p.m.
    ... Choosing the promoter Mature siRNAs typically range from 19–22 nucleotides and are 100% complementary... Taxman, D. J., Moore, C. B., Guthrie, E. H., & Huang, M. T. (2010). Short hairpin RNA (SHRNA): Design...

  7. Viral Vectors 101: Integrase-Deficient Lentiviral Vectors

    Type
    Blog Post
    Published
    May 13, 2025, 1:15 p.m.
    ...for researchers looking to generate long-lasting changes in cells. However, for some applications, integration...construct has the D64V mutation, a single amino acid change (from aspartic acid to valine at position 64) which...

  8. How to Negotiate a Successful Lab Start Up Budget

    Type
    Blog Post
    Published
    Sept. 1, 2015, 2:30 p.m.
    ...techs’ financial books.  To find personnel salary ranges, go to online job information sites (like, indeed.com...focused on research relevant objectives and their tangible resources. For example, if a dean/chair asks “...

  9. Antibodies 101: Multiplex Immunofluorescence

    Type
    Blog Post
    Published
    May 23, 2023, 1:15 p.m.
    ...palette for display (like green-magenta-blue or orange-blue rather than red-green). Even better, show ..., Ferling, I., Gazda, M. A., Hoeppner, L. H., Ilangovan, V., Lo, H., Olson, M., Mohamed, S. Y., Sarabipour...

  10. Hot Plasmids: Spring 2025

    Type
    Blog Post
    Published
    June 3, 2025, 1:15 p.m.
    ... a wide variety of genomic edits — single-base changes, small deletions, and large insertions up to 12... B., Tran, T., … Dimidschstein, J., Fishell, G., Wang, Y. (2024). An enhancer-AAV toolbox to target and...

  11. PiggyBac-ing Through the Genome Editing Field

    Type
    Blog Post
    Published
    May 31, 2016, 3:30 p.m.
    ...s chromatin structure undergoes significant rearrangement (such as when a stem cell initiates differentiation...researchers to avoid making unnecessary base pair changes as would be the case with ssODNs. One minor drawback...

  12. Plasmids 101: Using Transposons in the Lab

    Type
    Blog Post
    Published
    July 6, 2021, 1:28 p.m.
    ...McClinntock who first discovered transposons due to changes in coloration of corn kernels. Aside from corn,... et al., 2021). Target site preference and host range are important to consider when selecting a transposon...

  13. Transferable Skills Guide: Teamwork

    Type
    Blog Post
    Published
    Nov. 28, 2017, 2 p.m.
    ...Make one yourself. Instead of thinking “What’s my angle on attacking this problem?”, try thinking “Who can...can I assemble to attack this problem from every angle?” Your science will be better for it. Additional...

  14. Quick Guide to All Things Lentivirus

    Type
    Blog Post
    Published
    March 21, 2017, 2:30 p.m.
    ...retroviral vectors because they target a broader range of cells and their integration into the genome is...you put in your culture. As viral production can change from one batch to another. For experimental consistency...

  15. Plasmids 101: Modular Cloning Applications and Kits

    Type
    Blog Post
    Published
    May 14, 2024, 1:15 p.m.
    ...service (Lee et al., 2015). This kit offers a broad range of parts, including copy number machinery, chromosomal...elements such as promoters or fluorophores are interchangeable across organisms. When using the MK system,...

  18. CRISPR Guide Update

    Type
    Blog Post
    Published
    Oct. 10, 2024, 1:15 p.m.
    ... to use CRISPR plasmids in the lab and the vast range of possibilities at their disposal. With this update...
Showing: 501 - 520 of 705 results