We narrowed to 798 results for: Arc
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TypeBlog PostPublishedJan. 28, 2014, 3:10 p.m....coli Transformation Kit and Buffer Set from Zymo Research to make competent cells because cells prepared...shock. Detailed protocols are available via Zymo Research. In brief, we grow our E. coli in LB to log phase...
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Quick, Versatile Plant Transgenesis with GreenGate Plasmids
TypeBlog PostPublishedJan. 23, 2014, 3:23 p.m....A few years ago, researchers introduced what’s been called a major breakthrough in cloning technology...GreenGate – offers all of those same advantages to researchers working in plant model systems. “The cloning ... -
Editor's Choice, September 2016
TypeBlog PostPublishedOct. 7, 2016, 4 p.m....Addgene Director of Biology, Lianna Swanson, and researcher at Sanofi Pharmaceuticals. This post highlights... Addgene's Cancer Collection Pages Connect the Research Community Grad School Advice Part 1: Picking a... -
What's New in CRISPR - December 2019
TypeBlog PostPublishedDec. 17, 2019, 2:37 p.m....will help you find more new CRISPR tools for your research! This time: CRISPRi in Burkholderia Base editors...lentivirus-like particle. Read the paper in Nucleic Acids Research Find the gRNA and packaging plasmids Cytosine... -
Editor's Choice, August 2016
TypeBlog PostPublishedSept. 2, 2016, 1:32 p.m....you might expect with any nascent technology, researchers are having trouble getting NgAgo genome editing...successes, and to find out what delivery techniques researchers are using. The detailed results of his survey... -
Scientist Networking: What is an Informational Interview?
TypeBlog PostPublishedJuly 1, 2014, 3:42 p.m....interesting work. Consider this to be like any other research project. You’ve met someone whose career interests...Informational Interview is a great next step in your research. What an informational interview is not If ... -
Seeing Red: Simple GFP Photoconversion
TypeBlog PostPublishedAug. 4, 2015, 2:30 p.m....Since the first research applications of GFP were published in the 1990s, biologists have spent a lot... cells, and it may find wide use in biological research. Photoconvertible fluorescent proteins Newly... -
Easing the Protein Purification Process with pCri
TypeBlog PostPublishedJune 19, 2015, 3:08 p.m.... purification specialists recommend doing some research, notably determining the subcellular localization...for cloning (see schematic below), enabling a researcher to test multiple tags for one protein quickly... -
Three Tips to Organize Your Lab Notebooks in the New Year
TypeBlog PostPublishedJan. 2, 2014, 3:44 p.m....) to make it easier to know where to start your search. Then identify the most frequently used pages in...checklists that link to other notes, and use the search bar to quickly find what you’re looking for. Tip... -
IBEX Knowledge-Base: A Data Resource for Multiplex Tissue Imaging
TypeBlog PostPublishedJune 8, 2023, 1:15 p.m....representing imaging from 47 different tissues. Researchers upload and share both their successes (recommended...detergents that they used in their experiments. Researchers can then visit the IBEX imaging community discussion... -
Who Gives a Tweet? 9 Facts About Scientists on Twitter
TypeBlog PostPublishedJune 26, 2017, 1:31 p.m.... a scientist). Profiles and Twitter lists were searched for scientific titles generated from the United...Twitter. Or maybe scientist prefer to share links to research that isn’t behind paywalls. 5. The #1 (re)tweeted... -
Three Key Considerations for Precise Neuronal Targeting Using AAV Technologies
TypeBlog PostPublishedNov. 27, 2019, 2:03 p.m....wealth of genetically encoded tools available, researchers are also developing ways to improve delivery ...launched AAV Data Hub, an open platform where researchers using AAV from our viral service can share experimental... -
Celebrating Outstanding CRISPR/Cas9 Achievements at the Dr. Paul Janssen Award Dinner
TypeBlog PostPublishedSept. 15, 2014, 6:05 p.m....Charpentier and Doudna, who talked about their research leading up to the discovery of the mechanism of...Charpentier advocated for the crucial role of basic research in the scientific community and the importance... -
SpyLigase Irreversibly Locks Peptides Together for Efficient Cell Capture
TypeBlog PostPublishedAug. 13, 2014, 3:49 p.m....Proceedings of the National Academy of Sciences in March, he and his colleagues took another important step... Academy of Sciences paper [published online on March 17] should cover it. Addgene: OK, thanks for your... -
We're Updating Our Hold for Publication Policy
TypeBlog PostPublishedJune 24, 2025, 1:15 p.m....We all know the timeline for publishing research papers can be long and unpredictable. Fortunately, you...that aren’t distributed or for our teams to keep searching, emailing, and waiting for replies. This updated... -
CRISPR 101: Drug discovery and CRISPR
TypeBlog PostPublishedMay 4, 2023, 1 p.m....tissue types, and across species, it enables researchers to select and generate physiologically relevant... it’s an exciting time to be in the biomedical research field! References and Resources References Fellmann... -
Hot Plasmids - October 2022
TypeBlog PostPublishedOct. 6, 2022, 1:15 p.m.... HcKCRs represent a long-sought tool for researchers to control K+ gradients and can be used as a ...of the most commonly used protein tags - a quick search for ‘Myc tag’ in the Addgene catalog pulled up ... -
FastCloning: A Newer, Simpler Cloning Technique
TypeBlog PostPublishedNov. 1, 2016, 2:30 p.m....constitute foundational elements of biomedical research. Traditional restriction cloning is one of the...Resources on Addgene.org Find Empty Vectors for Your Research Find Additional Protocols Browse Our Special Collections... -
CRISPR 101: Cas9 Nickase Design and Homology Directed Repair
TypeBlog PostPublishedMarch 15, 2018, 12:59 p.m....By mutating one of two Cas9 nuclease domains, researchers created the CRISPR nickase. Nickases create a...editing. For more information and a link to the archived webinar, click here. We would like to thank IDT... -
New CRISPR Web Resources and #12DaysofCRISPR Recap
TypeBlog PostPublishedDec. 12, 2017, 3:13 p.m....high-quality educational resources to help you do better research. CRISPR is an incredibly fast-moving field, and...overview of the development of CRISPR into a varied research toolbox, as well as additional resources to help...