We narrowed to 798 results for: Cre
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TypeBlog PostPublishedJan. 26, 2016, 3:30 p.m....percentage of dystrophin-expressing muscle cells increases over time, and all three groups confirm dystrophin...muscle stem cells are modified by this approach, increasing the possibility that edits will persist long-...off-target risk must be evaluated separately, increasing the time to clinical approval. Even with the ...
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Scientific Reproducibility - Focusing on Solutions at the Minisymposium on Reproducibility
TypeBlog PostPublishedMay 18, 2018, 7:32 p.m....should be able to apply someone else's new and incredibly useful technique simply by following the abbreviated...49:45 - 1:18:45), there are also initiatives to create more reliable reagents for future research. I’ve...IPI is working to address this issue directly by creating well characterised antibodies against all human... -
Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands
TypeBlog PostPublishedMarch 21, 2024, 1:15 p.m....fluorescent dyes, and fluorescent ligands. Figure created with BioRender.com. Fluorescence refers to...fluorescent protein tags, such as higher specificity, increased versatility in probe attachment, and compatibility...exciting the fluorophore at peak wavelength can also decrease the rate of photobleaching. Interaction with receptors... -
Antibody Validation for Flow Cytometry
TypeBlog PostPublishedSept. 10, 2024, 1:15 p.m....of antibody validation, and it is recommended to screen multiple candidate antibodies when possible. If...interest. By correlating your data sets, you can increase confidence that the labeling observed represents...experiment. We find it useful to start with a screening procedure in easily genetically modified cell ... -
The CRISPR Software Matchmaker: A New Tool for Choosing the Best CRISPR Software for Your Needs
TypeBlog PostPublishedNov. 3, 2015, 3:30 p.m....is a significant hurdle made worse by an ever increasing marketplace. Since 2013 about 11 tools have been...the user is expected to interact with the tool (a screen shot and overview of the table can be found above...re engaged in heavy CRISPR design (I’m talking screens and/or very large genomes), then it is worthwhile... -
Hot Plasmids Podcast Episode 3: Optogenetics Tools, Improved CRISPR Base Editors, & More!
TypeBlog PostPublishedOct. 25, 2017, 1 p.m....for controlling protein activity, methods for increasing CRISPR editing efficiency, new CRISPR base editors...Segment Hot Plasmid 2: Ouabain selection for increased CRISPR editing efficiency. Listen to the Segement... -
Addgene is Stopping Use of X (formerly Twitter)
TypeBlog PostPublishedJan. 11, 2024, 2:15 p.m....platform changes, such as limiting access and increased priority given to paid content regardless of user...preserve the knowledge, conversations, and community created and shared on our feed, we will not delete @addgene... -
It’s CRISPR Week at Addgene!
TypeBlog PostPublishedMay 13, 2019, 12:01 p.m....various CRISPR applications (such as visualize, screen, activate, and repress) on Thursday. We’d also ...from nickases to base editing to pooled library screening. It also has information for planning your CRISPR... -
Lucky Thirteen: Guest Contributors of 2023
TypeBlog PostPublishedDec. 5, 2023, 2:15 p.m....celebrations! We're kicking it off by sharing all the incredible guest posts and stories featured in our blog ...Plasmid Request Data Author: Joachim Goedhart Created in collaboration with Addgene writers High Complexity... -
Congrats to the 2019 Deck The Lab Winners
TypeBlog PostPublishedJan. 17, 2020, 3 p.m....and Instagram and we’re excited to share these creative entries as the winners of the 2019 contest. ...material tree We often get submissions of decorations created from lab materials. This year, one of the winners... -
The Codon Guide to Synthetic Biology
TypeBlog PostPublishedSept. 14, 2023, 1:15 p.m.... guide that curates this information. And so I created the Codon Guide to Synthetic Biology in the hopes...open-access resources whenever possible. Image credit: Niko McCarty; adapted from Bruce Wetzel and Harry... -
Imaging Tools and Following a Passion for Basic Biology: Interview with Joachim Goεdhart
TypeBlog PostPublishedMarch 9, 2018, 2 p.m....at the University of Amsterdam. In addition to creating and sharing many great fluorescent protein tools...probes and more. Chapter 5 - mTurquoise II and the creation of improved fluorescent proteins through mutagenesis... -
8 Great Depositor Research Stories
TypeBlog PostPublishedDec. 15, 2022, 2:15 p.m....blogger Ava Shipman explains how the Wu lab is screening over 3,000 antibodies for use in mice and human...from 2022! Wow! Our depositors do some really incredible science - I hope you've enjoyed learning about... -
Introducing Addgene's Microbiology Pages
TypeBlog PostPublishedJune 13, 2016, 7:08 p.m....Microbiology Collection are sorted by organism (see screenshot above). Don’t see your favorite organism listed...Addgene has so many of these resources that we created filters just for them on our search page. Use the... -
#DeckTheLab with Tinsel and Test Tubes, Fa La La La La La La La La
TypeBlog PostPublishedDec. 2, 2021, 2:15 p.m....can stage in a lab diorama. Or maybe you want to create a stack of wrapped gifts using life science catalog...Twitter and Instagram shortly after. Have fun, be creative, and enjoy the end of year festivities - however... -
2018 #DeckTheLab Contest Winners
TypeBlog PostPublishedJan. 15, 2019, 2:19 p.m....materials into holiday decorations. We appreciated the creative uses of old posters, pipette tip boxes, and more...working on our reports/lab stuff for a few minutes to create this lovely winter scene out of stuff we found ... -
Editor's Choice, July 2016
TypeBlog PostPublishedAug. 5, 2016, 3 p.m....fluorophores other than fluorescent proteins can be incredibly useful in FRET experiments. Professor Fessenden's... also really helped put this technology into a concrete context. I recommend this post to anyone wondering... -
The Addgene Antibody Data Hub
TypeBlog PostPublishedSept. 12, 2024, 1:15 p.m....Data reports Each antibody submission is used to create a data report, which contains experimental data...the experimental conditions. Figure 4: A screenshot of the materials and methods section for the ... -
Bringing Sustainable Practices to the Lab: Easy Action Items
TypeBlog PostPublishedApril 22, 2019, 12:26 p.m....incorporate to reduce your lab consumption and increase your lab sustainability. For my other posts on...Subscribe to the Addgene blog Learn about other creative approaches for lab sustainability Resources on... -
2022 and 2023: Reflections and Looking Forward
TypeBlog PostPublishedFeb. 23, 2023, 2:15 p.m.... doesn’t. Reports receive a DOI for citation, crediting researchers when their data is used by the community...understand our role in opening up science to help create new knowledge and research opportunities. I have...