We narrowed to 805 results for: Cre

...made use of mazF as a counterselectable marker to create unmarked genetic modifications in the yeast Pichia...counter selection in one plasmid, they were able to create an efficient cloning tool. The plasmid pKSCTMF ...Morin and James Kaper used the hok/sok system to create strains that can stably express antigens in sufficient...

...expression transposases leading to increased integration frequency. (C) Increasing rounds of transfer on transposition-inducing...chromosomal integration of cargo without using screening markers. Capability to construct a strain library...

...each project. This creates accountability on the part of each developer and creates trust amongst the ... Addgene grows and evolves, we will be here to increase all of our teams’ efficiency and productivity....

...just might personally hand your credentials to HR, greatly increasing your chances of getting a phone ...can use to better prepare for your career, and increase your chances of landing that next coveted job!...

...glutathione reductase to faciliate proper folding; may increase multimer formation Δ(ara-leu)7697 ΔlacX74 ΔphoA...salts may be preferable if the protein product is secreted to the medium or if slow expression is required...required due to solubility concerns. Insoluble and Secreted Proteins: The most common purification protocols...

...you need to speak to start creating slides or thinking about content. Create a Google.slides or Powerpoint...capture interesting key results, striking images, creative ideas, and engaging stories as you encounter or...

...percentage of dystrophin-expressing muscle cells increases over time, and all three groups confirm dystrophin...muscle stem cells are modified by this approach, increasing the possibility that edits will persist long-...off-target risk must be evaluated separately, increasing the time to clinical approval. Even with the ...

...should be able to apply someone else's new and incredibly useful technique simply by following the abbreviated...49:45 - 1:18:45), there are also initiatives to create more reliable reagents for future research. I’ve...IPI is working to address this issue directly by creating well characterised antibodies against all human...

...fluorescent dyes, and fluorescent ligands. Figure created with BioRender.com. Fluorescence refers to...fluorescent protein tags, such as high specificity, increased versatility in probe attachment, and compatibility...exciting the fluorophore at peak wavelength can also decrease the rate of photobleaching. Interaction with receptors...

...of antibody validation, and it is recommended to screen multiple candidate antibodies when possible. If...interest. By correlating your data sets, you can increase confidence that the labeling observed represents...experiment. We find it useful to start with a screening procedure in easily genetically modified cell ...

...is a significant hurdle made worse by an ever increasing marketplace. Since 2013 about 11 tools have been...the user is expected to interact with the tool (a screen shot and overview of the table can be found above...re engaged in heavy CRISPR design (I’m talking screens and/or very large genomes), then it is worthwhile...

...for controlling protein activity, methods for increasing CRISPR editing efficiency, new CRISPR base editors...Segment  Hot Plasmid 2: Ouabain selection for increased CRISPR editing efficiency. Listen to the Segement...

...various CRISPR applications (such as visualize, screen, activate, and repress) on Thursday. We’d also ...from nickases to base editing to pooled library screening. It also has information for planning your CRISPR...

...platform changes, such as limiting access and increased priority given to paid content regardless of user...preserve the knowledge, conversations, and community created and shared on our feed, we will not delete @addgene...

...celebrations! We're kicking it off by sharing all the incredible guest posts and stories featured in our blog ...Plasmid Request Data Author: Joachim Goedhart  Created in collaboration with Addgene writers High Complexity...

...and Instagram and we’re excited to share these creative entries as the winners of the 2019 contest. ...material tree We often get submissions of decorations created from lab materials. This year, one of the winners...

... guide that curates this information. And so I created the Codon Guide to Synthetic Biology in the hopes...open-access resources whenever possible.  Image credit: Niko McCarty; adapted from Bruce Wetzel and Harry...

...at the University of Amsterdam. In addition to creating and sharing many great fluorescent protein tools...probes and more. Chapter 5 - mTurquoise II and the creation of improved fluorescent proteins through mutagenesis...

...blogger Ava Shipman explains how the Wu lab is screening over 3,000 antibodies for use in mice and human...from 2022! Wow! Our depositors do some really incredible science - I hope you've enjoyed learning about...

...Microbiology Collection are sorted by organism (see screenshot above). Don’t see your favorite organism listed...Addgene has so many of these resources that we created filters just for them on our search page. Use the...