We narrowed to 514 results for: Emb
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TypeBlog PostPublishedSept. 22, 2020, 1:15 p.m....backgrounds. In fact, many of my AAAS STPF cohort members came to DC to do either a sabbatical year or transition...
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Quick Guide to All Things Lentivirus
TypeBlog PostPublishedMarch 21, 2017, 2:30 p.m....liposome) or electrical (electric current forming membrane pores) transfection. These techniques are still... -
An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency
TypeBlog PostPublishedDec. 17, 2020, 1:30 p.m....perfectly viable at 15 ºC, yet exhibit complete embryonic lethality at 25 ºC. This method resulted in efficiencies... -
Typing CRISPR Systems
TypeBlog PostPublishedMarch 18, 2025, 1:15 p.m....fact have a helicase domain due to a gene that resembles cas10. All type IV subtypes are missing adaptation... -
Fluorescent Proteins 101: Monitoring Cell Mobility Using Fluorescent Proteins
TypeBlog PostPublishedAug. 15, 2017, 1:24 p.m.... cell mobility are essential processes during embryonic development. These are required for a growing ... -
Custom CRISPR Screens & the Green Listed Software
TypeBlog PostPublishedJuly 11, 2017, 2:30 p.m....screen. Currently, eleven published libraries are embedded. These were kindly donated by six different research... -
Antibodies 101: Designing Your First Flow Panel
TypeBlog PostPublishedMay 28, 2024, 1:45 p.m....expression, CD45 is incorporated into the plasma membrane and can be detected through a cell surface label... -
A Primer on Optogenetics: Introduction and Opsin Delivery
TypeBlog PostPublishedSept. 10, 2015, 2:30 p.m....at a similar spectrum as HR and also results in membrane hyperpolarization but unlike HR, this is achieved... -
CRISPR 101: Cytosine Transversion Editors
TypeBlog PostPublishedMarch 25, 2025, 1:15 p.m....Base Editing. Advanced Science (Weinheim, Baden-Wurttemberg, Germany), 9(26), e2202957. https://doi.org/10.1002... -
How to Deposit Your Plasmids with Addgene
TypeBlog PostPublishedFeb. 27, 2024, 2:15 p.m....possible. Note that the full plasmid sequence can be assembled or theoretical — it does not have to be entirely... -
Prime Editing: Adding Precision and Flexibility to CRISPR Editing
TypeBlog PostPublishedJan. 13, 2025, 9:49 p.m....October 2019 and updated by Emily P. Bentley in December 2024. References and Resources References Anzalone... -
Antibody Validation for Flow Cytometry
TypeBlog PostPublishedSept. 10, 2024, 1:15 p.m....workshops. Let’s use TIM-1 (Single pass type-1 membrane protein), which has been designated CD365 in the... -
CRISPR 101: Cytosine and Adenine Base Editors
TypeBlog PostPublishedFeb. 13, 2025, 2:15 p.m....ribonucleoprotein particles (RNPs) to both zebrafish embryos and the mouse inner ear. Fourth-generation cytosine... -
Using AAV for Neuronal Tracing
TypeBlog PostPublishedAug. 9, 2018, 1:04 p.m.... natural RABV or HSV can). AAV does not have a membrane envelope like lentivirus, but instead is contained...