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We narrowed to 925 results for: Gene

Showing: 501 - 520 of 925 results

  1. Celebrating Outstanding CRISPR/Cas9 Achievements at the Dr. Paul Janssen Award Dinner

    Type
    Blog Post
    Published
    Sept. 15, 2014, 6:05 p.m.
    ...they have been able to use CRISPR/Cas9 to generate mice with genes knocked out in the insulin signaling cascades...and Dr. Emmanuelle Charpentier. I was joined by Addgene Scientist Matt Ferenc (one of our resident CRISPR... to a year or greater with older technologies. Addgene is proud to be helping get CRISPR reagents out ...  New to CRISPR/Cas9 Technology? Learn More: Addgene's CRISPR/Cas9 Guide History of CRISPR Cas - A tale...

  2. Plasmids 101: Dimers and Multimers

    Type
    Blog Post
    Published
    Jan. 10, 2023, 2:15 p.m.
    ...CGE), which has been used in plasmid vaccine and gene therapy development to examine plasmid topologies...plasmid DNA concatemers in mammalian cells. J Gene Med. 2009 May;11(5):444-53. doi: 10.1002/jgm.1310. PMID: ...resources on Addgene.org  Addgene's Plasmids 101 eBookAddgene's Molecular Biology ReferenceAddgene's Restriction...length of the monomer (~11.6 kb). Image generated by SnapGene software.  Thanks to increasing affordability...RA, James AA, Kolodner R. recA-independent general genetic recombination of plasmids. Nature. 1981 Nov... performing restriction digests on plasmids at Addgene, we sometimes observe something odd in our uncut... the short-read Illumina MiSeq sequencing that Addgene uses.) However, when some researchers received ...

  3. Behind-the-scenes of the Isolation of the Thermostable IgnaviCas9 From a Yellowstone Hot Spring

    Type
    Blog Post
    Published
    Nov. 12, 2019, 2 p.m.
    ...Neddersen characterized a few samples by 16S rRNA gene sequencing which showed the presence of many “dark...Yellowstone National Park (YNP). Extremophiles - phylogenetically diverse microbes living in extreme physical...lab and ThermoCas9 from the van der Oost lab. Phylogenetic comparison indicates that like GeoCas9 and ThermoCas9...particularly useful. Additional resources on the Addgene blog Learn about other Cas proteins Read all of...GeoCas9, another thermostable Cas9 Resources on Addgene.org Read our CRISPR guide Find CRISPR plasmids for...

  4. Bright Monomeric Fluorescent Proteins: mNeonGreen, mTFP1, and mWasabi

    Type
    Blog Post
    Published
    April 25, 2019, 3:01 p.m.
    ...Oregon created mTFP1 by transforming E. coli with a gene library of approximately 484 variants, including... your research Subscribe to the Addgene blog Resources on Addgene.org Learn about the Michael Davidson...Allele Biotechnology that now can be deposited at Addgene. What makes these fluorescent proteins unique and...was then screened through successive rounds of mutagenesis for the brightest teal fluorescent proteins that...scientists from Florida State University, they used a mutagenesis approach and found that specific amino acid changes...scientific community, please deposit the plasmids at Addgene! References Ai, Hui-wang, et al. "Directed evolution...PMCID: PMC3811051. Additional resources on the Addgene blog Read our fluorescent protein blog posts Find...

  6. Antibodies 101: Affinity Tags

    Type
    Blog Post
    Published
    Sept. 19, 2023, 1 p.m.
    ...Solubility Enhancer. In P. E. Vaillancourt, E. coli Gene Expression Protocols (Vol. 205, pp. 99–118). Humana...-59259-301-1:99 Resources on Addgene.org Addgene antibodies Addgene’s antibody guide Antibody protocols...having solubility issues!). Need a GST antibody? Addgene has you covered! His His tags, often called polyhistidine...Purification of His fusion proteins from E. coli is generally more straightforward and of a higher purity than...and other qualities of each tag class, they are generally best suited for their primary application. Ready...protocols  Resources on the Addgene blog Plasmids 101: Protein tags Antibodies 101: Epitope tags To Each...

  7. What the HEK?

    Type
    Blog Post
    Published
    April 18, 2023, 1:15 p.m.
    ...affect factors that could skew research results (gene expression, growth rates, etc.). Thus, it’s important...present in this line, making it genetically female. HEKs are generally cultured as an adherent monolayer...plasmids with the SV40 origin of replication. To generate a virus, you need only transfect the ‘helper’ .../fbioe.2021.796991 Additional resources on the Addgene blog Five Popular Model Organisms 10 Tips for...and Troubleshooting Additional resources on Addgene.org AAV Production in HEK-293T Cells  ...

  8. Why Add Sucrose? Improved Yields for Adeno-associated Virus Preparation

    Type
    Blog Post
    Published
    Dec. 13, 2018, 1:14 p.m.
    ...during adeno-associated virus manufacturing." Human gene therapy21.10 (2010): 1251-1257. PMID: 20649475. ...have probably heard about Addgene’s viral service but did you know that Addgene also performs independent...101 eBook Additional resources on Addgene.org Learn about Addgene's viral service Learn about viral production...research? Since launching the viral service in 2016 Addgene’s research team has been actively investigating ...PMCID: PMC2957237. Additional resources of the Addgene blog Read our beginner's guide to viral vectors...

  9. Bricking Science: Portraying Scientific Reality Through LEGO

    Type
    Blog Post
    Published
    Nov. 29, 2016, 3:30 p.m.
    ...by the nearly infinite regulatory capabilities of gene pathways just waiting to be discovered. As we started...Bricking Science.      Additional Resources on the Addgene Blog Learn How You Can Use Video to Share Your... 10 Ways to Share Your Science Resources on Addgene.org Find Protocols and Protocol Videos Check out...

  10. Tips for Improving Your Next Manuscript

    Type
    Blog Post
    Published
    Nov. 22, 2016, 3:30 p.m.
    ...at East Tennessee State University. He works with gene expression in Pseudomonas aeruginosa. Follow him...Reading, & Organizing Write for the Addgene Blog Resources on Addgene.org Check out Our Revamped Protocol...laborious, but helped build my understanding of how to generate a manuscript. At the end of the online course,.... Over time that portion of the paper will be generated. After you complete a publication quality figure...figure. Writing a scientific manuscript is like generating a quilt with your lab mates, you add the individual...100percentSDS.     Additional Resources on the Addgene Blog Dos & Don'ts When Publishing a Scientific...

  11. Hot Plasmids - September 2019 - CRISPR, Auxin Inducible Degradation, MerMAID Channelrhodopsins, and Genome Looping

    Type
    Blog Post
    Published
    Sept. 3, 2019, 1:15 p.m.
    ... role in the transcriptional regulation of many genes. A number of groups have designed means of studying...with mAID. Using this method, the lab was able to generate conditional alleles of a few essential proteins... al., 2019). Find the mAID tagging toolkit at Addgene Yesbolatova et al., Methods, 2019. PubMed PMID:...targeted by the Cas9 nuclease. The Streptococcus pyogenes Cas9 (SpCas9) requires an NGG PAM to recognize...difficult. Find SpCas9 and Target-AID-NG plasmids at Addgene! Nishimasu H et al., Science, 2018. PubMed PMID... MerMAIDs - marine channelrhodopsins for the optogenetic toolbox Article contributed by Susanna Bachle...quickly and with high precision - a valuable optogenetics feature when studying neuronal activity. The...

  12. Science Careers: Unruly Interests Feed Many Paths

    Type
    Blog Post
    Published
    Sept. 26, 2017, 12:12 p.m.
    ...regulation of DNA replication and gene transcription during vertebrate embryogenesis. My interests, though, have...Career Blog Posts Resources at Addgene.org Find Job Openings at Addgene ...than what fits for applied research. I focus in general terms on a couple of very broad topic domains, ...Pam_Hines and LinkedIn. Additional Resources on the Addgene Blog What Do I Do Now? Academic v. Non-Academic...

  13. Visualizing Translation at the Single Molecule Level

    Type
    Blog Post
    Published
    Aug. 1, 2017, 1:15 p.m.
    ... to examine translation of Drosophila patterning gene oskar. Early in development, the osk-TRICK reporter...Find plasmids from this paper at Addgene. Additional Resources the Addgene Blog Learn More about SunTag... remained elusive. Two techniques developed by Addgene depositors have made it easier to track translation... on the nascent polypeptide recruit scFV-GFP, generating green puncta that colocalize with the red reporter...PMC2746483 Find ribosome profiling plasmids at Addgene. 2. Halstead, James M., et al. “Translation. An...: PMC4451088 Find plasmids from this paper at Addgene. 3. Yan, Xiaowei, et al. “Dynamics of Translation... Brightest Fluorescent Protein Resources on Addgene.org Find Fluorescent Protein Plasmids Check Out ...

  14. Cultivating Community Science at BosLab

    Type
    Blog Post
    Published
    Nov. 2, 2017, 1:35 p.m.
    ...journal club discussion on Kevin Esvelt’s CRISPR/Cas9 gene drive paper was very popular. People kept asking... at Addgene.org Order Plasmids from Addgene! Find Free and Open Educational Resources at Addgene! ...BosLab. Once he optimizes plant cell culture and transgenesis he wants to share his knowledge with the community...microbes. We’ve ordered a few yeast plasmids from Addgene and are eager to do our first yeast transformations... @ZebraElement     Additional Resources on the Addgene Blog Experimenting in an Open Source Lab: from...

  15. The Crispr Babies Saga Shows the Need for Action, Not More Delays

    Type
    Blog Post
    Published
    Dec. 20, 2018, 1:15 p.m.
    ...China was using CRISPR/Cas9 in an attempt to create gene-edited babies. Several hours later, the scientist...public consensus on genome editing, there was a generally agreed-upon international moratorium on utilizing...incorrectly known as “three-parent babies,” where fused genetic material from intended parents is “rehoused” in...decisions on scientific, ethical, regulatory, and general public consensus to the next meeting of experts...and the entire 2018 saga of “what’s happening in genetics this time?” She lives with her partner (in business...on theirpenmanship. Additional resources on the Addgene blog Find all of our CRISPR blog posts Download... genome engineering blog posts Resources on Addgene.org Read our CRISPR guide Find CRISPR plasmids by...

  16. Plasmids 101: How to Verify Your Plasmid Using a Restriction Digest Analysis

    Type
    Blog Post
    Published
    Aug. 28, 2014, 3:34 p.m.
    ...Congratulations, you have a plasmid expressing your gene of interest (YGOI) and are ready to dive into your...received matches the expected sequence. Here at Addgene, we use NGS-based quality control to confirm the...necessary but also an easy process. Please vist Addgene's resource for plasmid verification to find additional...DNA gel.  More Plasmid Eductional Resources: Addgene's Molecular Biology Reference Find More Protocols...

  17. CRISPR 101: Multiplex Expression of gRNAs

    Type
    Blog Post
    Published
    Sept. 10, 2020, 11:45 a.m.
    ...sequence between two target sites. Modifying multiple genes at once. Using multiplexed gRNAs can target multiple...simultaneous multiplex editing of six different genes. This system doesn’t rely on any additional or host-dependent...from this paper at Addgene. 9. Yin, Linlin, et al. “Multiplex Conditional Mutagenesis Using Transgenic ...plasmids from this paper at Addgene. Additional Resources on the Addgene Blog Browse Our CRISPR Topic...add multiple gRNAs on one plasmid. Sound good? Addgene has many tools to help you multiplex - we’ll use...multiplex gRNAs, some being: Using dual nickases to generate a knockout or edit. This can help reduce off-target...Multiplexing gRNAs: the basics One common question Addgene Senior Scientists receive is: can I express more...

  18. Opto-Nanobodies: Using Light to Manipulate Cell Signaling and Protein Purification

    Type
    Blog Post
    Published
    Nov. 19, 2019, 2:08 p.m.
    ...telephone, until the message reaches the nucleus where gene expression changes lead to cell proliferation and... on Addgene.org Find nanobody expression plasmids here Learn more about optogenetics in Addgene's optogenetics...induced binding. The lab used this general design scheme to generate two more OptoNBs, one against mCherry...loop 1 always generated OptoNBs with dark-induced binding, but insertion into loop 6 generated both blue ...on the key components of OptoNBs, how they are generated, and their use for protein purification and controlling...nanobody’s ability to bind its target protein.  Generating OptoNBs The opening and closing of the AsLOV2...spatiotemporal resolution than pharmacological or genetic manipulation of the signaling pathway.  As a proof-of-principle...

  19. Fluorescent Protein Travel Awards - Protein Variants, a Serotonin Sensor, and an Artificial Leaf Replica System

    Type
    Blog Post
    Published
    June 4, 2019, 1:02 p.m.
    ...that control cell cycle gene expression.  Additional Resources on the Addgene Blog Visit the fluorescent...work in Lin Tian’s lab at UC Davis is to generate a genetically encoded fluorescent sensor for serotonin...sequence an individual’s genome. But when they find genetic variants, what does that mean? Previous research...of Washington sought to address the effects of genetic variants in a high throughput manner. She helped...modeling, directed evolution, site-saturation mutagenesis, and medium-throughput screening, she increased...GFP Find microbiology blog posts Resources on Addgene.org Find fluorescent protein biosensors Browse Plasmids...

  20. Visualizing Genomic Loci with CRISPR-Sirius

    Type
    Blog Post
    Published
    April 18, 2019, 12:53 p.m.
    ... observing low-copy genomic loci or single copy genes. If you want to visualize genomic loci brightly,...PP7, or boxB sequences inserted into the sgRNA generates blue, green, or red colors for labeling. A combination... PP7, or boxB sequences in the same sgRNA will generate additional colors. Image from Ma et al., 2016 ...CRISPR-Sirius a try. Find CRISPR-Sirius plasmids at Addgene. References Ma, Hanhui et al. “Multicolor CRISPR...PubMed PMID: 30377374. Additional resources on the Addgene blog Download our CRISPR eBook Learn about fluorescent...tools Read all CRISPR blog posts Resources on Addgene.org Browse our CRISPR plasmids for visualization...
Showing: 501 - 520 of 925 results