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Showing: 501 - 520 of 727 results

  1. Plasmids 101: Plasmid Incompatibility

    Type
    Blog Post
    Published
    March 31, 2020, 1:15 p.m.
    ...high for both plasmids, so each plasmid “thinks” it has a higher copy number than it actually does, and neither...large, low copy virulence plasmids. This strategy has been successful in displacing, or “curing” the virulence...

  2. Antibodies 101: Introduction to Immunofluorescence

    Type
    Blog Post
    Published
    Nov. 16, 2021, 2:15 p.m.
    ...target is known to be absent naturally or in which it has been knocked-out/knocked-down. “No primary”, pre-... reliable in the future.  We hope that this post has shed some light on immunofluorescence. The technique...

  3. Viral Vectors 101: Pseudotyping

    Type
    Blog Post
    Published
    April 20, 2021, 2:30 p.m.
    ...Cytotoxicity can also be cell type dependent: if a cell has few receptors for a specific envelope protein, it...lentiviral vector to be transported retrograde and also has higher neuronal transduction efficiency and a better...

  4. An Introduction to Adenovirus

    Type
    Blog Post
    Published
    Oct. 3, 2023, 1:15 p.m.
    ... virulence (Tollefson et al., 1996) – cell death has been reported when using vectors, and immune inactivation...the infection to sometimes result in cell death, has led to interest in using adenoviral vectors for vaccine...

  5. Tips for Getting a Faculty Position

    Type
    Blog Post
    Published
    July 20, 2023, 1:15 p.m.
    ...eBook, How to Get a Faculty Position. This edition has substantial expansions on the chalk talks and Zoom...I also incorporated example documents. This part has generated a lot of positive feedback. Many of these...

  6. CRISPR 101: Homology Directed Repair

    Type
    Blog Post
    Published
    Jan. 26, 2023, 2:15 p.m.
    ...applicable in DSB-induced genome engineering, it has biological importance for the repair of breaks that...directed repair and genome engineering CRISPR-Cas9 has revolutionized the genome engineering world and made...

  7. CRISPR-based Genome Editing Systems For Editing in E. coli

    Type
    Blog Post
    Published
    April 13, 2021, 1:15 p.m.
    ...editing systems in bacteria. Our pCas/pTargetF system has been one of the most popular genomic editing systems...Yang lab.   In conclusion, the MUCICAT system has the following advantages: Fast and site-specific...

  8. Getting the Most from Your Lentiviral Transduction

    Type
    Blog Post
    Published
    April 7, 2016, 2:30 p.m.
    ...mycoplasma contamination. Mycoplasma contamination has been shown to arise from a variety of sources such...line for mycoplasma and discard it if the culture has become overly confluent or too old. Ensuring that...

  10. Addgene's Website Update

    Type
    Blog Post
    Published
    April 8, 2024, 2:15 p.m.
    ...soon as the homepage opens. You’ll notice it now has two options: “Search Repository” and “Deposit Materials...

  11. 500,000 Plasmids Shipped and Counting!

    Type
    Blog Post
    Published
    March 26, 2015, 3:24 p.m.
    ...2004 to today, you can see how plasmid distribution has grown. Additionally, more and more scientists outside...

  12. Science Communication for Everyone

    Type
    Blog Post
    Published
    April 16, 2019, 1:05 p.m.
    ...cornerstone for The Addictive Brain. Our community has also requested live question and answer sessions ...

  18. Using Phosphoserine to Study Protein Phosphorylation

    Type
    Blog Post
    Published
    June 23, 2016, 2:30 p.m.
    ...is important to determine how much phosphorserine has actually been incorporated into the full-length protein...protein. Up to 80% p-Ser incorporation into E17TAG GFP has been achieved with the Rinehart system (6); however...

  19. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

    Type
    Blog Post
    Published
    May 10, 2016, 2:30 p.m.
    ...tough job! Kamel Khalili’s lab at Temple University has demonstrated two potential strategies for CRISPR-...approach might be more successful. “Shock and kill” has the advantage of killing cells infected with HIV-...
Showing: 501 - 520 of 727 results