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We narrowed to 889 results for: IND

Showing: 501 - 520 of 889 results

  1. When is a Monomer not a Monomer? The Top Three Ways Your Favorite Fluorescent Protein Oligomerizes in Cells

    Type
    Blog Post
    Published
    April 19, 2016, 2:30 p.m.
    ...Fluorescent Protein Resources Find Other Plasmids that Glow (Luciferase Plasmids) Find Optogenetics Resources...protein. A dimeric fusion protein will have two FP binding domains forming a building block sufficient to ...your studies and greatly lower your chances of finding artifactual results. Many thanks to our guest ...Addgene Blog Read Professor Snapp's Post on mOX FPs Find the Best Fluorescent Protein for Your Needs Get ...

  2. Sharing Your Lab Protocols: Using Apps to Save Time & Track Your Experiments

    Type
    Blog Post
    Published
    Sept. 23, 2014, 3:05 p.m.
    ...month it’s been viewed by users in the US, China, India, Canada, the UK, South Korea, and beyond!   Check...use, and add your own comments. Currently you can find protocols from NEB, various academic labs, and now...classic protocols on Protocol Online that you might find useful for your research. LabGuru Website: http:...pricing options for academic labs, departments, industry, and more to fit every lab's needs. Plus there...offers software designed with scientists workflow in mind and can offer a one-stop solution to your lab's ...

  3. Which Fluorescent Protein Should I Use?

    Type
    Blog Post
    Published
    May 20, 2014, 2:06 p.m.
    ...available FP emission colors and 10 points to keep in mind when choosing an FP (or two) for your upcoming experiments... used are GECIs - genetically encoded calcium indicators (e.g. GCaMP). Others include: pHluorin & pHTomato...Fluorescence Complementation (BiFC). 8 points to keep in mind when choosing a fluorescent protein Excitation ...considered when labeling proteins that interact. Indeed, FRET is often used to determine if two proteins...isolated from the Unagi eel was shown to mature independently of oxygen, making suitble for use in anaerobic...

  4. Great Results Start with Great Standard Curves

    Type
    Blog Post
    Published
    Aug. 15, 2024, 1:15 p.m.
    ...rely on standard curves in their day-to-day work. Indeed, viral vector production facilities frequently ...diluted in a series ranging from 1:2 to 1:32. Each individual step of the series is consistent at 1:2. When... from 0 to 1 with 1 being a perfect fit and 0 indicating that there is no linear relationship between ...the %CV of the standard curve, one averages the individual %CVs for each point on the curve. ‌The smaller...complex trendlines. If you don’t have access to this kind of software, don’t give up! Rather than plotting...

  5. Getting to Know Expansion Microscopy

    Type
    Blog Post
    Published
    June 20, 2023, 1:15 p.m.
    ...the inspiration behind ExM, how it works, and some of the limitations to keep in mind whether you are ... within ~250 nm of each other. For reference, individual presynaptic terminals, which are jam packed with...the equipment needed for EM is not something you find in the average cell and molecular biology lab, and...their relative positions, allowing you to resolve individual points with a conventional, diffraction-limited...

  6. CRISPR 101: Making a Knock-In Cell Line

    Type
    Blog Post
    Published
    Dec. 22, 2022, 2:15 p.m.
    ...donor get used as a template for repair? Read on to find out! Homology-directed repair and genome engineering... to form ssDNA and uses the ends of the ssDNA to find a repair template. The highest HDR efficiency is...clones by reading up on validating genome edits to find screening strategies for your experiment. Also learn...homology-directed repair choice in response to CRISPR/Cas9 Induced-double strand breaks. Int J Mol Sci., 21(18): 6461...Resources on Addgene.org Browse All CRISPR Plasmids Find Validated gRNAs for Your Next Experiment Check out...

  7. Lentiviral Vector Uses and Overview

    Type
    Blog Post
    Published
    May 19, 2016, 2:30 p.m.
    ...Andre K, Stein D, Blick G, Greenberg RN, Kinder C, Zolopa A, Binder-Scholl G, Tebas P, June CH, Humeau LM...addition of a chimeric 5’ LTR containing a Tat-independent promoter. Although safer, this system may be ...839-48. PubMed PMID: 26758916. 7. Transfusion independence and HMGA2 activation after gene therapy of human...Our Lentivirus Guide Learn about Virus Biosafety Find Lentiviral Vectors for Your Research  ...

  8. Minigenomes - a Safe Way to Study Dangerous Viruses Like the Ebola Virus

    Type
    Blog Post
    Published
    March 17, 2016, 2:30 p.m.
    ...Therefore, the roles/functions of individual proteins can be studied independently of other viral proteins simplifying...this would prevent the study of the proteins independently from each other (see Uses of Minigenome System...luciferase can readily be measured to quantify (indirectly) transcription performed by the viral polymerase...easily studied as each component can be examined independently. In addition, experiments can be performed in...

  9. Plasmids 101: Protein tags

    Type
    Blog Post
    Published
    Dec. 11, 2014, 4:26 p.m.
    ...calmodulin binding peptide (CBP), a TEV cleavage site (more on that in a moment), and 2 ProtA IgG-binding domains...with folding and stabilization, as can maltose-binding protein (MBP). Overexpression can also lead to ...KRRWKKNFIAVSAANRFKKISSSGAL 4 Affinity and Purification Binding and elution steps use very moderate buffer conditions...proteases without requiring the addition of an independent cleavage recognition site. In fact, SUMO cannot...

  10. How to Write a Scientific Review Article

    Type
    Blog Post
    Published
    Feb. 16, 2017, 3:30 p.m.
    ...I mentioned, I wasn’t an expert in the field. To find reviews, I just searched online and found ones that...well-known researchers in the field. I made sure to find information from genome-wide studies, as well as...you need to read every paper from start to finish. Find the information that’s relevant to you, note it,...could see how big the studies were, and you could find the original paper if you wanted to learn more. ...look done, just keep working on it. Also bear in mind that this is just a review article and not your ...

  11. What's Your Organism? Expanding Genomic Tools via the NSF EDGE Program

    Type
    Blog Post
    Published
    May 2, 2019, 12:41 p.m.
    ... everyone. Addgene is certainly going to have to find ways to tag and curate reagents from these emerging...emerging organisms so scientists can easily find the useful new reagents that come out of this work as the...parenting strategies and make important ecological indicators since species live in water and on land. Current...nests so can be studied over time. They recognize individual faces (I guess of other wasps) so can be used... expensive. They are using a cool fluorescent indicator system to rapidly identify recombinants. Arthropods...

  12. Designing Your Chalk Talk for the Academic Job Interview

    Type
    Blog Post
    Published
    March 12, 2019, 1:37 p.m.
    ...committee to understand your goal, what you expect to find, and how you will interpret and use the information... correct or your data favor a different model. Finding evidence for or against a model is a standard scientific...knowledge gap? This includes the overview and the individual aims. Describe the question, the bare minimum...know they will work. Stabilize your blood sugar. Find an energy bar that you like and consider eating ...photographer.  Additional resources on the Addgene blog Find tips for presenting a science talk Check out our...

  13. To Codon Optimize or Not: That is the Question

    Type
    Blog Post
    Published
    Nov. 12, 2020, 2:15 p.m.
    ...synthesis relies on transfer RNAs (tRNAs). tRNAs bind to and deliver amino acids to the ribosome where...complementary to the mRNA sequence. The 3’ end of  the tRNA binds the amino acid that corresponds to the anticodon...1980s, scientists even created a codon adaptation index (CAI) that is based on the codon usage frequency...https://doi.org/10.1038/nsmb1031 Gustafsson C, Govindarajan S, Minshull J (2004) Codon bias and heterologous...nrg3051 Sharp PM, Li W-H (1987) The codon adaptation index-a measure of directional synonymous codon usage ...

  14. Twenty Years of Sharing: Addgene's Viral Vector Service

    Type
    Blog Post
    Published
    July 30, 2024, 1:15 p.m.
    ...Addgene’s 20th anniversary celebration, we’ve been finding and sharing stories unique to our repository and...biosensors were distributed in 2023 alone. You’ll also find a large number of requests for vectors in the “controls...available in the repository. GCaMP, a calcium indicator (biosensor, by Addgene’s categorization) was first...community they serve. As difficult as it may be to find answers to such questions, Addgene is deeply interested...will continue to do so, in the hopes that these findings can help others as much as they've helped us.  ...

  15. Viral Vectors 101: Pseudotyping

    Type
    Blog Post
    Published
    April 20, 2021, 2:30 p.m.
    ...different levels of cytotoxicity and the mechanisms behind the cytotoxicity can vary. For example, the popular...virus G protein. VSV-G is a trimeric protein that binds phosphatidylserine and low-density lipoprotein receptors...the potential for off-target infectivity as it can bind and transduce many cell types before reaching the...needs. Check out some of these review articles to find tables and information on different viral membranes... on Addgene.org: Check out the lentivirus guide Find ready-to-use lentiviral preps at Addgene ...

  16. Using Video to Share Your Science: We Share Science

    Type
    Blog Post
    Published
    Nov. 3, 2016, 2:30 p.m.
    ... to everyday obstacles for many of us, such as finding time to read all of these articles. Most of us ...research. So how can we fault others for failing to find our work?  I study processes for diagnosing and ...where you can see the yearly interdisciplinarity index of research for single or multiple disciplines –... more, you can then link to the journal article, find blogs about the study, or even review Facebook and...aquaculture/ The goal of the WeShareScience project is to find new and exciting ways to share ideas derived from...

  17. Changing Labor Laws Bring Increased Postdoc Wages

    Type
    Blog Post
    Published
    Oct. 25, 2016, 2:30 p.m.
    ...information for postdoctoral positions is very hard to find and this is partly due to the administration of ...paid. Institutional salary policies are one indicatator of how salaries may changes due to the FLSA. ...Current salary information that we have been able to find suggests that salaries may even double. The University...to the new level:  We are still waiting for an indication of what (most of) Harvard, Johns Hopkins, (most...make gradual adjustments towards this new level. Indeed some institutions did this. It doubtless took a...

  18. The Challenges of Cell Culture

    Type
    Blog Post
    Published
    May 5, 2016, 2:30 p.m.
    ...Proliferation and Invasion in Renal Cancer Cells, Marker-independent identification of glioma-initiating cells. The...that can impede collaborations, especially with industry. Today we also have the appropriate tools to verify... standards and has laboratories to carry our independent quality control for a range of testing, from ...forensics, DNA sequencing to drug testing. You can find Nick on LinkedIn. References 1. Skloot, Rebecca,... Protein Localization Resources on Addgene.org Find Plasmids for Mammalian Cells ...

  19. Getting the Most from Your Lentiviral Transduction

    Type
    Blog Post
    Published
    April 7, 2016, 2:30 p.m.
    ...other important binding events take place during the course of infection. These binding events can be impaired...infect and select, seems simple, in actuality, many find using lentivirus to be time consuming, difficult...possible. One problem that often plagues labs and hinders experiments is mycoplasma contamination. Mycoplasma...Lentiviral Vectors Additional Resources on Addgene.org Find Viral Vectors for Your Research Brush Up on All ...

  20. Important Considerations in Optogenetics Behavioral Experiments

    Type
    Blog Post
    Published
    Oct. 1, 2015, 2:30 p.m.
    ...nose-poke at a particular hole (the active hole) induces laser stimulation of the chosen brain circuit, .... If activation of the circuit is sufficient to induce reinforcement (instills a sense of “reward”), then...software such as EthoVision. Consult the literature to find all the applications of optogenetics relevant to...region may affect the animal; stimulation could induce seizures or some other unwanted phenomenon or it...learn optogenetics is straight through the source - find a lab within your own neuroscience sub-discipline...
Showing: 501 - 520 of 889 results