We narrowed to 893 results for: IND

...develop new working relationships with these individuals who often had different styles from their predecessors...laid out plan of action, others would act more independently and some others would need regular re-direction...organization speaks in “one voice” whilst among individual members, many opinions may exist. I learnt to...Becoming involved is not too difficult and you can find opportunities around you. You just need to be prepared...Twitter @JulietAdvocSci.        Further Reading Find more Career articles on the Addgene Blog Read Joanne...

... I’m not going to lie, AAV transfer plasmids are kind of a pain to work with. These plasmids contain ITRs...digests. I freeze the bacterial pellet for the remainder of the cultures and later maxiprep only the cultures...18265393. Additional resources on the Addgene blog Find out what makes AAV a versatile tool for gene expression...calculator for planning AAV packaging transfections Find tips for a first time CRISPR user Resources on ...and lentivirus Check out Addgene's virus protocols Find AAV for your research ...

...techniques Resources on Addgene.org Find basic molecular biology protocols Find plasmid cloning protocols Check...upon growth on LB/Cam/X-gal/IPTG agar plates, indicating successful reconstruction of the coding sequence...assembly that would result in a blue phenotype. Indeed, this was seen in all lacI/ lacZ assembly test ... efficiencies and accurate assembly levels were indeed possible, with correct, in-frame assembly proceeding...

... suits discussing multimillion dollar contracts behind closed doors. Fig. 1: The mythical land ...get what I want, that means you don’t. In this mindset, one of us wins, the other loses. Although this...examples depend on many specific details about the individuals, each example provides a scenario where both ...to you being remembered as a person who tries to find solutions that benefit everyone. Even if I “lose...positively if they tried to understand my position and to find a creative win-win solution. What does professional...

..., Ras proteins have stumped the pharmaceutical industry for years. They have proven very difficult to ... lack areas that are accessible, high-affinity binding targets for potential therapeutic agents. Nevertheless...demonstrated that a number of novel small molecules can bind directly, albeit weakly, to K-Ras and inhibit its... in human cancers, and synthesizing compounds to bind specifically to their mutated residues. For Ras ...Sun, Qi, et al. "Discovery of small molecules that bind to K‐Ras and inhibit Sos‐mediated activation." Angewandte...

...Fluorescent Protein Resources Find Other Plasmids that Glow (Luciferase Plasmids) Find Optogenetics Resources...protein. A dimeric fusion protein will have two FP binding domains forming a building block sufficient to ...your studies and greatly lower your chances of finding artifactual results. Many thanks to our guest ...Addgene Blog Read Professor Snapp's Post on mOX FPs Find the Best Fluorescent Protein for Your Needs Get ...

...month it’s been viewed by users in the US, China, India, Canada, the UK, South Korea, and beyond!   Check...use, and add your own comments. Currently you can find protocols from NEB, various academic labs, and now...classic protocols on Protocol Online that you might find useful for your research. LabGuru Website: http:...pricing options for academic labs, departments, industry, and more to fit every lab's needs. Plus there...offers software designed with scientists workflow in mind and can offer a one-stop solution to your lab's ...

...available FP emission colors and 10 points to keep in mind when choosing an FP (or two) for your upcoming experiments... used are GECIs - genetically encoded calcium indicators (e.g. GCaMP). Others include: pHluorin & pHTomato...Fluorescence Complementation (BiFC). 8 points to keep in mind when choosing a fluorescent protein Excitation ...considered when labeling proteins that interact. Indeed, FRET is often used to determine if two proteins...isolated from the Unagi eel was shown to mature independently of oxygen, making suitble for use in anaerobic...

...donor get used as a template for repair? Read on to find out! Homology-directed repair and genome engineering... to form ssDNA and uses the ends of the ssDNA to find a repair template. The highest HDR efficiency is...clones by reading up on validating genome edits to find screening strategies for your experiment. Also learn...homology-directed repair choice in response to CRISPR/Cas9 Induced-double strand breaks. Int J Mol Sci., 21(18): 6461...Resources on Addgene.org Browse All CRISPR Plasmids Find Validated gRNAs for Your Next Experiment Check out...

...rely on standard curves in their day-to-day work. Indeed, viral vector production facilities frequently ...diluted in a series ranging from 1:2 to 1:32. Each individual step of the series is consistent at 1:2. When... from 0 to 1 with 1 being a perfect fit and 0 indicating that there is no linear relationship between ...the %CV of the standard curve, one averages the individual %CVs for each point on the curve. ‌The smaller...complex trendlines. If you don’t have access to this kind of software, don’t give up! Rather than plotting...

...the inspiration behind ExM, how it works, and some of the limitations to keep in mind whether you are ... within ~250 nm of each other. For reference, individual presynaptic terminals, which are jam packed with...the equipment needed for EM is not something you find in the average cell and molecular biology lab, and...their relative positions, allowing you to resolve individual points with a conventional, diffraction-limited...

...Andre K, Stein D, Blick G, Greenberg RN, Kinder C, Zolopa A, Binder-Scholl G, Tebas P, June CH, Humeau LM...addition of a chimeric 5’ LTR containing a Tat-independent promoter. Although safer, this system may be ...839-48. PubMed PMID: 26758916. 7. Transfusion independence and HMGA2 activation after gene therapy of human...Our Lentivirus Guide Learn about Virus Biosafety Find Lentiviral Vectors for Your Research  ...

...Therefore, the roles/functions of individual proteins can be studied independently of other viral proteins simplifying...this would prevent the study of the proteins independently from each other (see Uses of Minigenome System...luciferase can readily be measured to quantify (indirectly) transcription performed by the viral polymerase...easily studied as each component can be examined independently. In addition, experiments can be performed in...

...calmodulin binding peptide (CBP), a TEV cleavage site (more on that in a moment), and 2 ProtA IgG-binding domains...with folding and stabilization, as can maltose-binding protein (MBP). Overexpression can also lead to ...KRRWKKNFIAVSAANRFKKISSSGAL 4 Affinity and Purification Binding and elution steps use very moderate buffer conditions...proteases without requiring the addition of an independent cleavage recognition site. In fact, SUMO cannot...

...synthesis relies on transfer RNAs (tRNAs). tRNAs bind to and deliver amino acids to the ribosome where...complementary to the mRNA sequence. The 3’ end of  the tRNA binds the amino acid that corresponds to the anticodon...1980s, scientists even created a codon adaptation index (CAI) that is based on the codon usage frequency...https://doi.org/10.1038/nsmb1031 Gustafsson C, Govindarajan S, Minshull J (2004) Codon bias and heterologous...nrg3051 Sharp PM, Li W-H (1987) The codon adaptation index-a measure of directional synonymous codon usage ...

...different levels of cytotoxicity and the mechanisms behind the cytotoxicity can vary. For example, the popular...virus G protein. VSV-G is a trimeric protein that binds phosphatidylserine and low-density lipoprotein receptors...the potential for off-target infectivity as it can bind and transduce many cell types before reaching the...needs. Check out some of these review articles to find tables and information on different viral membranes... on Addgene.org: Check out the lentivirus guide Find ready-to-use lentiviral preps at Addgene ...

...I mentioned, I wasn’t an expert in the field. To find reviews, I just searched online and found ones that...well-known researchers in the field. I made sure to find information from genome-wide studies, as well as...you need to read every paper from start to finish. Find the information that’s relevant to you, note it,...could see how big the studies were, and you could find the original paper if you wanted to learn more. ...look done, just keep working on it. Also bear in mind that this is just a review article and not your ...

... everyone. Addgene is certainly going to have to find ways to tag and curate reagents from these emerging...emerging organisms so scientists can easily find the useful new reagents that come out of this work as the...parenting strategies and make important ecological indicators since species live in water and on land. Current...nests so can be studied over time. They recognize individual faces (I guess of other wasps) so can be used... expensive. They are using a cool fluorescent indicator system to rapidly identify recombinants. Arthropods...

...committee to understand your goal, what you expect to find, and how you will interpret and use the information... correct or your data favor a different model. Finding evidence for or against a model is a standard scientific...knowledge gap? This includes the overview and the individual aims. Describe the question, the bare minimum...know they will work. Stabilize your blood sugar. Find an energy bar that you like and consider eating ...photographer.  Additional resources on the Addgene blog Find tips for presenting a science talk Check out our...

...Addgene’s 20th anniversary celebration, we’ve been finding and sharing stories unique to our repository and...biosensors were distributed in 2023 alone. You’ll also find a large number of requests for vectors in the “controls...available in the repository. GCaMP, a calcium indicator (biosensor, by Addgene’s categorization) was first...community they serve. As difficult as it may be to find answers to such questions, Addgene is deeply interested...will continue to do so, in the hopes that these findings can help others as much as they've helped us.  ...