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  1. London Calling - Addgene’s New Europe Office in London

    Type
    Blog Post
    Published
    June 10, 2014, 3:20 p.m.
    ...collaboration. This is why she is excited to be working at Addgene, because it brings scientists together...others. This love of interacting with people and talking about science makes her the perfect Addgenie and...

  2. Lentiviral CRISPR Libraries Enable Genome-Scale, Knockout Screening

    Type
    Blog Post
    Published
    Feb. 20, 2014, 4:57 p.m.
    ...technology has really changed the game for anyone looking to quickly and easily manipulate specific genes...These pooled libraries include enrichment against kinases, cell cycle proteins, and more.  “In the past, ...

  3. An Addgene Summer Internship

    Type
    Blog Post
    Published
    Aug. 8, 2024, 7:22 p.m.
    ...her talk about Addgene’s internship opportunity taking place next summer.  I applied to Addgene and began...week at Addgene. This report shared my work by breaking down the process of each day, my background, observations...

  4. Plasmids 101: Gene, enhancer, and promoter trapping

    Type
    Blog Post
    Published
    May 2, 2023, 1 p.m.
    ...not be expressed in the tissue/cell line you are working with. Development-specific genes – if a gene...depends on your experiment and the organism you are working in. The spacing of genes and whether or not it’...

  5. Hot Plasmids - October 2022

    Type
    Blog Post
    Published
    Oct. 6, 2022, 1:15 p.m.
    ...selectivity for K+ over Na+ and rapid photocurrent kinetics. They demonstrate the use of HcKCR1 as an optogenetic...photocurrents, and is more selective for K+ than HcKCR2, making it more suitable for in vivo optogenetic use.   ...

  8. Addgene at the ASM Microbe 2016 Conference

    Type
    Blog Post
    Published
    July 7, 2016, 2:30 p.m.
    ... to improve our resources for this community. Networking with microbiologists Over the course of the conference... technology. Addgene got several shout-outs for making popular screening libraries like Brie and Brunello...

  9. Management for Scientists: Managing vs. Leading

    Type
    Blog Post
    Published
    March 31, 2015, 4:08 p.m.
    ... be. Managers must ensure that their teams are working well together and collaborating effectively with...ability to assess and initiate things independently. Taking initiative doesn’t mean going rogue, pointing out...

  10. Management for Scientists: Giving Feedback

    Type
    Blog Post
    Published
    Feb. 6, 2015, 2:46 p.m.
    ...some people find this hard so get used to it by making it a concrete goal to say “thank you, job well ... they are at risk of being fired and should be looking for a new position. One of the most common mistakes...

  11. Antibodies 101: Epitope Tags

    Type
    Blog Post
    Published
    May 11, 2023, 1:15 p.m.
    ...application. Depending on the tag, directly imaging or tracking your protein may also be possible, without the...also be removed by treating the protein with enterokinase, which recognizes the 5 amino acids on the C...

  12. Antibodies 101: Chimeric Antibodies

    Type
    Blog Post
    Published
    April 25, 2023, 1:15 p.m.
    ...You are a scientist looking to determine how Protein A and Protein B interact. You read extensive research...Robinson, M. K., Graham, E. T., Sabo, J. P., Owens, R., King, D. J., Norris, S. H., & Scher, D. S. (1994). Isotype...

  13. What the HEK?

    Type
    Blog Post
    Published
    April 18, 2023, 1:15 p.m.
    ... four. No Y chromosome is present in this line, making it genetically female. HEKs are generally cultured... line for you. Are HEKs for you?   If you are looking for a human cell line model, HEKs might be a great...

  14. Summer SciComm: Science Storytelling

    Type
    Blog Post
    Published
    Sept. 16, 2022, 1:15 p.m.
    ...by outright stating the big-picture problem, by asking your audience a simple question, like “Have you...ending of the story.  At this point you may be thinking  “I don’t want to bury my results all the way ...

  15. Identifying Sequence Elements with SnapGene's Feature Database

    Type
    Blog Post
    Published
    Dec. 21, 2017, 2:06 p.m.
    ... days or even weeks. Primer design was done painstakingly by hand. Records of plasmid construction were...newly sequenced bacterial or viral strains. We are working on a way for SnapGene users to apply features from...

  16. Why Add Sucrose? Improved Yields for Adeno-associated Virus Preparation

    Type
    Blog Post
    Published
    Dec. 13, 2018, 1:14 p.m.
    ...sugar may be a more attractive choice for labs looking to improve their AAV yields. Given the wide range...Clathrin-mediated endocytosis and subsequent endo-lysosomal trafficking of adeno-associated virus/phage." Journal of ...

  19. Celebrate Open Data Day with Addgene’s AAV Data Hub!

    Type
    Blog Post
    Published
    March 5, 2020, 2:15 p.m.
    ... much-needed community resource. Instead of painstakingly searching for experimental details in research...currently still in beta version, so we’re always looking for ways to improve the viewing or data deposition...
Showing: 501 - 520 of 779 results