...stimulation. GPCR signaling GPCR signaling is a complicated process involving many downstream signaling mechanisms...applying optogenetic tools derived from direct comparative analysis of microbial opsins. Nature Methods,...

...Gene knockout with CRISPR technology is usually accomplished by Cas9-mediated dsDNA breaks: following a cut... accessibility due to chromatin status). No computational prediction is ever perfect, but this can decrease...to conduct genetic screens, however, a library composed of the latter would likely be more effective, ...

... a post-program self-assessment, participants completed follow-up self assessments and program evaluations...knowledge and skills had improved significantly when compared to their pre-program self-assesment. Achievements...other countries. In 2016, we expect the program to comprise over 2000 participants (mentors and mentees). ...

...transfer expert who understands the somewhat complicated Material Transfer Agreement process that kits...function. Project team A Project Team is assembled to complete a series of tasks with precisely defined goals...organization on the planet, in which case whoa! Mission accomplished! One example of a mission-based team in academia...

...In 1976, the world’s first genetic engineering company, Genentech, was founded.  This commercialization...instead of recognizing nucleotide triplets, the components recognize individual nucleotides. This imparts...intracellular pangenesis to the rediscovery of Mendel. Comptes Rendus de l’Académie des Sciences - Series III ...

...validation is so important. Antibodies are a critical component of the researcher toolkit due to their ability...will bind to - particularly when you start using complex samples such as whole cell lysate or tissue sections...BioRender.com.     Orthogonal Strategies This approach compares target expression of antibody-independent approaches...

...attached fluorophore. However, since tRNA suppression competes with natural chain termination at stop codons,...contrast can be improved using disulfide-containing compounds such as British anti-Lewisite (which was originally...Vilensky & Redman, 2003]). Most importantly, these compounds cross cell membranes, thus enabling FRET measurements...

... this segment Hot Plasmid 1: The Tol2 Gateway-Compatible Toolbox for quickly generating zebrafish transgenic.... Listen to Our Segement on the Tol 2 Gateway-Compatible Toolbox  Hot Plasmid 2: AAV Vectors for tageting...

...Get Tips on Verifying Your Plasmid Table 1: Comparing Typical Comericial Ladders with the PSU Ladders...1000 Lanes Cost $200-$500  <$10 $20-30 Cost Comparison: While convenient, commercial ladders cost between...

...experiment. Search engines let you easily find and compare available antibodies from many vendors, while data...Search engine May include validation data  Biocompare Any Any Search engine     Are we missing...

...multinational pharmaceutical company based in Switzerland. After completing her PhD in Chemistry and Chemical...

... 1. Lab B needs a redo :-( @SandTBioSci #CompetitiveStreaking @Addgene pic.twitter.com/mk44J4Gcj9 — Dave...species for the challenge, but one species always outcompeted the other species.  When he found the chromoprotein...

...genome usually for a research or applied goal. Components of CRISPR: gRNAs: Sequences of RNA that direct...single base changes in the DNA sequence without completely cutting the DNA. RNA Editors: CRISPR nucleases...

...decorated office! Below, they turned one of the lab computers into a fireplace scene.  Got our @EmoryChem ...all who participated in the 2018 #DeckTheLab competition. The four winners will receive a holiday care...

...neutralization or targeted destruction.  Antibodies are composed of two light chains and two heavy chains. The ...the use of less toxic adjuvants; 2) generating complex antibody chips that allow assessment of many protein...

...lab equipment on? This applies to water baths, computers, and more. Consolidate your autoclave runs. In...long-term goals to stay motivated and to feel accomplished. In addition to doing some of the quick things...

...measure will ever adequately capture a notion as complex as “scientific impact.” As we look ahead to the...Science - The Book Bollen J, et al. "A Principal Component Analysis of 39 Scientific Impact Measures" (2009...

...’s role in infection also makes it a critical component of vaccines and the target of neutralizing antibodies...Science Translational Medicine have made their complete collection of relevant papers freely available...

...optimal DNA cleavage at 48 °C, a temperature incompatible to genome editing in human cells. The Feng Zhang...efficiency can be controlled by manipulating complementarity between the gRNA and target. Read the paper...

...about careers that we’ve compiled our top career blog posts into one comprehensive, downloadable guide: the...