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We narrowed to 801 results for: Ran

Showing: 501 - 520 of 801 results

  1. Tips for Using BLAST to Verify Plasmids

    Type
    Blog Post
    Published
    May 29, 2014, 1:29 p.m.
    ...blastn), Standard Protein BLAST (blastp), and Translated BLAST (blastx). NCBI has a terrific getting-started...gaps in the nucleotide sequence, you can run a Translated BLAST (blastx) search to check for expected ORFs..., due to the additional searches involved in translating the nucleotide sequence into all six possible...choose for your sequencing result and can easily translate it, we recommend using blastp over blastx. The...

  2. The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data

    Type
    Blog Post
    Published
    July 16, 2020, 1:15 p.m.
    ...cases being reported (right, red), likely due to quarantining {% video_player "embed_player" overrideable...-examine our global data at a finer temporal granularity. For each paper, we observed that the demand ...imposed on scientists. Figure 6: Model fit. Orange line depicts observed data. Blue line is the sum...depending on when we believe the China COVID-19 date range is, they could be the most impacted or least impacted...

  3. Negative Can Be Positive: Open AAV Data with Addgene

    Type
    Blog Post
    Published
    Sept. 6, 2022, 1:15 p.m.
    ...expressing neurons and GFP (green) to label neurons transduced by each serotype. Working at Addgene has ...answers hundreds of AAV questions every year on a range of issues related to AAV vectors, many from people...S., & Klein, R. L. (2018). More expansive gene transfer to the rat CNS: AAV PHP.EB vector dose–response.../10.1038/s41434-018-0028-5 de Backer, M. W. A., Brans, M. A. D., Luijendijk, M. C., Garner, K. M., & Adan...

  4. MXS Chaining

    Type
    Blog Post
    Published
    Feb. 7, 2017, 3:30 p.m.
    ...visualize cell cycle dynamics, titrate inducible transgene expression, or a variety of other clever applications...expression) and a polyA signal (to terminate transcription) and the four constructs were then combined ...derived from the tyrosine-protein kinase Lyn) Membranes 3 mCherry 587nm/ 610 nm human β-Actin Actin ...their 6-mer sequences, are rarely found in the transcriptomes of these species and can often be used to clone...

  5. CRISPR Antimicrobials

    Type
    Blog Post
    Published
    May 3, 2016, 2:30 p.m.
    ...antibiotic resistance using multiple approaches. Major restaurant chains including Chipotle and Panera Bread have... joining machinery, so a CRISPR-induced double-stranded break (DSB) in the genome is lethal. If this DSB...diverse, and each phage has a narrow bacterial host range. To further potential clinical applications, Ando...Beisel, Chase L., Ahmed A. Gomaa, and Rodolphe Barrangou. “A CRISPR design for next-generation antimicrobials...

  6. The Importance of a Fun Workplace: Company Culture at Addgene

    Type
    Blog Post
    Published
    Sept. 3, 2015, 2:30 p.m.
    ... elaborately pranking each other, dressing in costume, and holding competitions (ranging from cook-offs...orderly and timely fashion. 9. Ask for help My grandma used the old adage “many hands make light work”..., evite, posters, megaphone, or your company’s intranet. Don’t forget about the power of simple word of...

  7. Supporting Reproducibility with a Connected ELN

    Type
    Blog Post
    Published
    Jan. 17, 2019, 1:38 p.m.
    ...that emerged from intensive engagement with a wide range of people at the University of Wisconsin in 2011...reproducibility an ELN should have integrations with a broad range of relevant generic and science-specific tools. ... however, in some cases it would be possible to grant those wanting to understand and/or attempt to reproduce...Massachusetts, and feels privileged to be part of the vibrant life sciences research and commercialization community...

  8. Antibodies 101: Immunoprecipitation

    Type
    Blog Post
    Published
    Dec. 7, 2021, 2:15 p.m.
    ... protein of interest, such as a sample transiently transfected or stably infected with the protein of ...downregulated, examine a protein’s stability or post-translational modifications, or study how a target protein...experiments using a tagged protein may not be translatable to the endogenous system and will need to be...

  9. Twenty Years of Sharing: Expanding Accessibility

    Type
    Blog Post
    Published
    Nov. 21, 2024, 10:47 p.m.
    ... package stays in transit for a certain number of days and tracking average transit time for each courier... often weren’t easily accessible, due to a wide range of logistical barriers. Addgene worked to identify...Protocols Agarose Gel Electrophoresis Bacterial Transformation How to Design a Primer Creating Bacterial Glycerol...

  10. AAV Titers: Where Do They Come From And What Do They Mean?

    Type
    Blog Post
    Published
    Nov. 15, 2017, 3 p.m.
    ...particles that can transduce cells. Infectious titers are typically quantified by cell transduction assays. Wild-type....qPCR...Digital droplet PCR...DNA dot blot...Transduction assay...SDS-PAGE...Electron microscopy… Any ...titer of a viral vector stock can vary based on transduction target and can also be altered by freeze-thaw...

  11. How to Write a Scientific Review Article

    Type
    Blog Post
    Published
    Feb. 16, 2017, 3:30 p.m.
    ...popular papers, but looked for papers from a wide range of authors. Just start writing When I first started...drafts of the manuscript. This is the time to transform the document into something cohesive-- change ...Thompson, and Thomas D. Gilmore. "Histone acetyltransferases and histone deacetylases in B-and T-cell ...Tips for Improving Your Next Manuscript 6 Tips for Grant Writing Resources on Addgene.org Check out Our...

  12. Plasmids 101: Gateway Cloning

    Type
    Blog Post
    Published
    Jan. 12, 2017, 3:30 p.m.
    ...sequencing or restriction digest! Then, you can transform or transfect the cells that you want to use for your... reactions are performed, the next step is to transform competent E. coli cells and select the positive...Cloning Technology: Advantages and Drawbacks. Clon Transgen 4:138. doi:10.4172/2168-9849.1000138 2. Hartley...

  13. Healthcare Consulting: A Door to the Business of Life Sciences

    Type
    Blog Post
    Published
    Oct. 13, 2016, 2:30 p.m.
    ...pharmaceutical companies, biotech start-ups, health insurance companies, and governments on the most effective...physicians and patients, shaping discussions with insurance companies about the pricing and coverage of drugs...therapeutic approach brings to patients, doctors, and insurance providers, and is crucial to how our clients’ ...When I was first considering preparing for and transitioning to a career in healthcare consulting, I found...

  14. Quick Guide to Near-Infrared Fluorescent Proteins

    Type
    Blog Post
    Published
    Oct. 5, 2021, 1:15 p.m.
    ...dDetermined as effective NIR fluorescence in transiently transfected live HeLa cells with no supply of exogenous...lamps are the best for working in this spectral range. You can also check that no filters and/or cold ...and after normalization to fluorescence of co-transfected EGFP. e Originally reported as a monomer16, IFP2.0...

  15. Filming Science Videos in the Age of Social Distancing

    Type
    Blog Post
    Published
    May 20, 2021, 1:15 p.m.
    ...looking, I found an inexpensive HDMI transmitter that could transmit a signal between a wall. There was ... things first was getting that wireless HDMI transmitter/receiver, to beam the camera’s image to my remote...then set up a monitor connected to the wireless transmitter and an iPad in the office next door (connected...

  16. Proximity Labeling: A Powerful Tool for Protein Complex Purification and Proteomic Mapping

    Type
    Blog Post
    Published
    Dec. 2, 2019, 2 p.m.
    ... avidin, proximity labelling can capture weak/transient protein interactions as well as be used for both...proximity labeling by promiscuous biotin ligases (Branon et al., 2018). BioID and BioID2 can take hours ...2019). This technique relies on an S. auerus transpeptidase sortase A variant (mgSrtA), which is capable...motif, give these methods a try.  References:  Branon, Tess C., et al. "Efficient proximity labeling ...

  17. Replacing Paper: Tips for Choosing an Electronic lab Notebook

    Type
    Blog Post
    Published
    July 10, 2018, 1:08 p.m.
    ...types of ELNs are available on the market today, ranging from general note taking tools, to more scientific....e. main priorities of different ELN features, ranging from whether respondents saw them as not important...requires time and knowledge. In a recent article, we translated our knowledge of electronic lab notebooks into...information. If an employee leaves the institution, the transition is smooth as all data is organized within an ...

  18. CRISPR 101: Off-Target Effects

    Type
    Blog Post
    Published
    Feb. 8, 2024, 2:15 p.m.
    ...While this may effectively serve as a transfection or transduction control, a different gRNA would have..., you’ll want to select a Cas enzyme with low tolerance for mismatches within the gRNA sequence. ...near each other, this will result in a DNA double strand break (DSB), the event required for frameshift ...

  19. A Guide to Getting Started in Undergrad Research

    Type
    Blog Post
    Published
    June 8, 2021, 1:15 p.m.
    ...investigator. In basic science (as opposed to translational, biomedical, or applied sciences), people conduct...don’t tell us everything there is to know is a strange and exciting revelation. Science in any field can...investigator (PI) Runs the lab Writes a lot of grants Varying degrees of engagement with what happens...1-2 years) Often a gap-year position taken to transition to the next career stage (ex: grad school, med...

  20. Custom CRISPR Screens & the Green Listed Software

    Type
    Blog Post
    Published
    July 11, 2017, 2:30 p.m.
    ... modified cells are transferred into an animal and the behavior of the transferred cells is followed. ...generating a massive amount of descriptive transcriptomics data. Custom CRISPR screens designed to target...pathway with many receptors, signaling molecules, transcription factors, and ligands. The presenter then showed...
Showing: 501 - 520 of 801 results