We narrowed to 612 results for: Sis

1. GCE4All: Making Genetic Code Expansion Accessible

   Type

   Blog Post

   Published

   June 15, 2023, 1:15 p.m.

   ...essentially those needed to make site-directed mutagenesis, many researchers new to GCE aren’t able to successfully...

2. Viral Vectors 101: The AAV Retrograde Serotype

   Type

   Blog Post

   Published

   April 27, 2023, 1:15 p.m.

   ... for understanding how the brain functions. The basis for these connections in the brain are (drum roll...

3. How Addgene Creates Content

   Type

   Blog Post

   Published

   Nov. 17, 2022, 2:15 p.m.

   ...reviews on blog posts are done on a case-by-case basis, as many of our blog contributors primarily write...

4. Antibodies 101: Buffers, Storage, and Conjugates

   Type

   Blog Post

   Published

   Feb. 1, 2022, 2:15 p.m.

   ...data in a manner that demands high accuracy and consistency?  If the answer to any of these questions is...

5. Multiplex Genome Editing with CRISPR-Cpf1

   Type

   Blog Post

   Published

   May 9, 2017, 2:12 p.m.

   ...For cloning, Zetsche et al used four oligos that consist of direct repeats and crRNA.  Similar to a jigsaw...

6. 15 Years of Addgene: The Top 15 Plasmids

   Type

   Blog Post

   Published

   Jan. 8, 2019, 1:54 p.m.

   ...customizable sgRNA from the U6 promoter and puromycin resistance from the EF-1a promoter. It’s a 3rd generation...

7. Hot Plasmids December 2018 - C. elegans RNAi, TurboID, Detecting Viral Outbreaks, and Protein Binding Specificity Tools

   Type

   Blog Post

   Published

   Dec. 6, 2018, 1:34 p.m.

   ...involved in cytoskeletal control, cell growth, and endocytosis. Recently, the Sidhu group at the University ...

8. Plasmids 101: Inducible Promoters

   Type

   Blog Post

   Published

   Jan. 18, 2018, 2:34 p.m.

   ...present, YFI is inactive, preventing repressor cI synthesis and allowing reporter gene transcription to take...

9. A Novice's Perspective on Neuroscience - SfN2017

   Type

   Blog Post

   Published

   Dec. 15, 2017, 2:07 p.m.

   ...seem to require some mathematical know-how for analysis. If you’re looking to get into neuroscience and...

10. Synthesized by Ginkgo Bioworks, Shared by Addgene: SARS-CoV-2 Plasmids for Many Expression Systems

    Type

    Blog Post

    Published

    May 19, 2020, 1:15 p.m.

    ...2008) Pseudotyped Vesicular Stomatitis Virus for Analysis of Virus Entry Mediated by SARS Coronavirus Spike...

11. Why and how to start studying chromatin (with Addgene's help)

    Type

    Blog Post

    Published

    June 12, 2018, 11:40 a.m.

    ...chromatin-related plasmid in a collection that grows on a daily basis, the lists below provide a snapshot of relevant ...

12. A Quick Guide to a Career in Software Product Management

    Type

    Blog Post

    Published

    Nov. 24, 2020, 2:15 p.m.

    ...current and future features being built for the analysis software program, and meet with the automation...

13. Plasmids 101: Screens vs. Selections

    Type

    Blog Post

    Published

    Nov. 10, 2022, 2:15 p.m.

    ...trying to identify genetic conditions which convey resistance to a drug. You could culture cells in the drug...

14. Hot Plasmids - May 2022

    Type

    Blog Post

    Published

    May 26, 2022, 1:15 p.m.

    ... live imaging application. Each visual barcode consists of a fluorescent protein coupled to a specific...

15. Building and Validating Recombinant Antibodies: A Case Study

    Type

    Blog Post

    Published

    April 5, 2022, 1:20 p.m.

    ...validate your new construct by Sanger sequence analysis. But for this research, it was likely that the...

16. Protein Tagging with CRISPR/Cas9: A Conversation with Mendenhall and Myers

    Type

    Blog Post

    Published

    July 28, 2015, 2:30 p.m.

    ...transcription factor. It also gives cells antibiotic resistance to neomycin so that you can select the population...

17. How to Make Friends and Meet People at a Scientific Conference

    Type

    Blog Post

    Published

    Aug. 7, 2014, 1:58 p.m.

    ... Conference Dinner - by Julio Peironcely on the Thesis Whisperer "Not Networking - Building Relationships...

18. 3 Challenges in Plant Synthetic Biology

    Type

    Blog Post

    Published

    July 22, 2014, 5:46 p.m.

    ...plant synthetic biology In addition to public resistance, an overall limited understanding of plant biology...

19. Advice for Starting a Biotech Company from a Grad Student turned Entrepreneur

    Type

    Blog Post

    Published

    July 10, 2014, 7:17 p.m.

    ...company won’t go anywhere. The team should initially consist of 2-3 individuals that are aligned in vision and...

20. Form Your Own Peer Mentoring Group: A How-To Guide for Scientists

    Type

    Blog Post

    Published

    Feb. 18, 2014, 4:22 p.m.

    ...programs. The groups in these programs typically consist of 1-2 mentors and 3-5 mentees who meet together...