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TypeBlog PostPublishedApril 9, 2020, 1:15 p.m....takes time away from our research. For all these reasons, the QCRG is delighted to be partnering with Addgene...
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Hot Plasmids - December 2019 - CRISPR pooled library, LipoGlo, CRISPRa, and Voltage Imaging Tools
TypeBlog PostPublishedDec. 10, 2019, 1:58 p.m....the mouse CRISPR knockout library at Addgene. Henriksson et al., Cell, 2019. PubMed PMID: 30639098. LipoGlo... -
FlipGFP, a novel fluorescence protease reporter to study apoptosis
TypeBlog PostPublishedMay 21, 2019, 12:10 p.m....reporters have been developed, such as the Forster resonance energy transfer (FRET)-based executioner caspase... -
The Crispr Babies Saga Shows the Need for Action, Not More Delays
TypeBlog PostPublishedDec. 20, 2018, 1:15 p.m....the research has been adequately proven to be reasonably safe and effective. We do that through a standardized... -
New and Upcoming Viral Vectors - September 2019
TypeBlog PostPublishedSept. 12, 2019, 1 p.m.... neurons near the target site.” pAAV-CamKIIa-ChrimsonR-mScarlet-KV2.1 (124651-AAV9) Recombinase AAV... -
Addgene Welcomes John Doench to the Advisory Board
TypeBlog PostPublishedAug. 13, 2015, 2:30 p.m....Addgene has behind it is something that very much resonates with me.” About Addgene’s role in the future,... -
Plasmids 101: How to Verify Your Plasmid Using a Restriction Digest Analysis
TypeBlog PostPublishedAug. 28, 2014, 3:34 p.m.... Lianna Swanson... -
Fluorescent Proteins 101: Luciferase
TypeBlog PostPublishedJune 24, 2014, 3:59 p.m.... Jason Niehaus... -
Quick Guide to Working with Drosophila Part 3: Genome Engineering in Flies
TypeBlog PostPublishedJuly 28, 2017, 1:30 p.m....chromosome. Targeted Insertion allows for direct comparisons between lines expressing different variants of... -
Luminescent Imaging with Nano-lanterns
TypeBlog PostPublishedMay 25, 2017, 2:30 p.m.... is based on the principle of bioluminescence resonance energy transfer (BRET). Photons emitted by a Renilla... -
Flow Cytometry Readouts: Yes, No, and Everything in Between
TypeBlog PostPublishedAug. 20, 2024, 1:15 p.m....population cluster would be shifted to the right in comparison, say somewhere between a 104–105 signal intensity... -
Viral Vectors 101: Biosensors
TypeBlog PostPublishedJuly 6, 2023, 1 p.m....monitor with biosensors? You can keep tabs on my personal favorite (the caffeine of the cell!)—ATP. There... -
GCE4All: Making Genetic Code Expansion Accessible
TypeBlog PostPublishedJune 15, 2023, 1:15 p.m.... Rachel Leeson... -
Viral Vectors 101: The AAV Retrograde Serotype
TypeBlog PostPublishedApril 27, 2023, 1:15 p.m....Haery in 2017 and significantly updated in 2023 by Jason Nasse. References 1. Tervo, D. Gowanlock R., et... -
Viral Vectors 101: Viruses as Biological Tools
TypeBlog PostPublishedMarch 21, 2023, 1:15 p.m....electroporation of a human, for example, isn’t a reasonable option. Since viruses used in labs are derived... -
Communicating Your Science With Help From ComSciCon
TypeBlog PostPublishedJuly 19, 2018, 1:12 p.m....mediated by some kind of communication to another person. In an interview setting, this means persuading... -
AAV Q&A with Tim Miles
TypeBlog PostPublishedMay 17, 2022, 1:15 p.m....thanks to our panel of experts, Tim Miles, David Goertson, Xinhong Chen, and Miggy Chuapoco, and to all ... -
GPCRs: How Do They Work and How Do We Study Them?
TypeBlog PostPublishedFeb. 11, 2021, 2:15 p.m....detect G protein activation via bioluminescence resonance energy transfer 2 (BRET2). Check out our blog ... -
A Quick Guide to a Career in Software Product Management
TypeBlog PostPublishedNov. 24, 2020, 2:15 p.m....UK) to help solve those problems. What type of person goes into software product management? I always... -
Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs
TypeBlog PostPublishedJune 2, 2015, 6:06 p.m....in the graphic below - including some simple comparisons. The easy multiplexing afforded by PTGs will...