We narrowed to 546 results for: TIL;

1. Finding nucleic acids with SHERLOCK and DETECTR

   Type

   Blog Post

   Published

   April 16, 2020, 1 p.m.

   ...bottleneck. In the last few years, scientists have utilized CRISPR-Cas9 protein variants, Cas13, and Cas12a...

2. New Tools Enable CRISPRa for Neuroscience Applications

   Type

   Blog Post

   Published

   July 2, 2019, 12:51 p.m.

   ...CRISPRa? Although cDNA overexpression approaches may still be preferable for individual gene activation in ...

3. Sonic Hedgehog? Sleeping Beauty? Learn About the Genes Behind Addgene’s Conference Room Names

   Type

   Blog Post

   Published

   April 2, 2019, 1:44 p.m.

   ...is critical for processes such as cell movement/motility, development, cytokinesis, and muscle contractions...

4. Designing Your Chalk Talk for the Academic Job Interview

   Type

   Blog Post

   Published

   March 12, 2019, 1:37 p.m.

   ...laundry list of molecules to screen has unclear utility. You cannot predict what will happen. Without the...

5. Supporting Reproducibility with a Connected ELN

   Type

   Blog Post

   Published

   Jan. 17, 2019, 1:38 p.m.

   ...well as the DSpace repository. These integrations utilize an interface that enables users to conveniently...

6. CRISPR-mediated Plant Base Editors

   Type

   Blog Post

   Published

   Jan. 3, 2019, 1:35 p.m.

   ...be applied to fix the defective base precisely? Until 2016, precise single base changes were only possible...

7. Deciphering the Mysteries of Behavior with Viral Vectors

   Type

   Blog Post

   Published

   Sept. 17, 2019, 12:40 p.m.

   ...Viral vectors, in contrast, are considerably more versatile and specific in how they manipulate neuronal function...

8. Bacterial Toxin-antitoxin Systems as Molecular Biology Tools

   Type

   Blog Post

   Published

   Nov. 1, 2018, 12:35 p.m.

   ...plasmid due to improper plasmid partitioning, it will still inherit some toxin and antitoxin. However, the antitoxin...

9. Plasmids 101: Codon usage bias

   Type

   Blog Post

   Published

   Sept. 27, 2018, 1:09 p.m.

   ...in the past (Angov et al., 2008), but there are still many more features to consider when designing a ...

10. Controlling for Off-target Effects with a New Genome-wide CRISPR Screen Design

    Type

    Blog Post

    Published

    Sept. 13, 2018, 1:55 p.m.

    ...exist to control for these faults. However, it’s still unclear if similar pitfalls exist for CRISPR screens...

11. CRISPR 101: Targeting RNA with Cas13a (C2c2)

    Type

    Blog Post

    Published

    Sept. 21, 2017, 2:07 p.m.

    ... processing in live cells. Indeed, groups have utilized dCas13 fusion proteins for imaging, tracking, ...

12. Using Video to Share Your Science: We Share Science

    Type

    Blog Post

    Published

    Nov. 3, 2016, 2:30 p.m.

    ...attention, though in reality much of our research still fails to reach our primary target audience – colleagues...

13. Changing Labor Laws Bring Increased Postdoc Wages

    Type

    Blog Post

    Published

    Oct. 25, 2016, 2:30 p.m.

    ...their salaries raised to the new level:  We are still waiting for an indication of what (most of) Harvard...

14. Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs

    Type

    Blog Post

    Published

    Oct. 27, 2015, 3 p.m.

    ... increase in fluorescent signal achieved when utilizing an optimized moxFP as opposed to standard FPs....

15. Plasmids 101: The Promoter Region – Let's Go!

    Type

    Blog Post

    Published

    April 3, 2014, 8:05 p.m.

    ...the cell, promoters themselves are controlled by still other regulatory factors: enhancers, boundary elements...

16. The Advances Behind The World's First Personalized CRISPR Treatment

    Type

    Blog Post

    Published

    Sept. 16, 2025, 1:15 p.m.

    ... in different patients. While these gRNAs would still need to be optimized and assessed for off-target...

17. Hot Plasmids: Summer 2024

    Type

    Blog Post

    Published

    Aug. 27, 2024, 1:15 p.m.

    ...overcome with methods such as chemical crosslinking or tilted-stage imaging. A truncated form of a tardigrade...

18. Antibodies 101: Introduction to Gating in Flow Cytometry

    Type

    Blog Post

    Published

    April 4, 2024, 1:15 p.m.

    ...following gates, cells that are not of interest can still be gated out later. Based on personal preference...

19. New Optogenetic Tools for Cytoskeleton and Membrane Control

    Type

    Blog Post

    Published

    Sept. 28, 2023, 1:15 p.m.

    ...tools to control formation of lamellipodia and contractile stress fibers, and recently developed a full ...

20. CRISPR 101: Homology Directed Repair

    Type

    Blog Post

    Published

    Jan. 26, 2023, 2:15 p.m.

    ...will keep cutting and repairing your target DNA until a mutation is introduced disrupting one or both ...