We narrowed to 546 results for: TIL;

1. Plasmids 101: Codon usage bias

   Type

   Blog Post

   Published

   Sept. 27, 2018, 1:09 p.m.

   ...in the past (Angov et al., 2008), but there are still many more features to consider when designing a ...

2. Controlling for Off-target Effects with a New Genome-wide CRISPR Screen Design

   Type

   Blog Post

   Published

   Sept. 13, 2018, 1:55 p.m.

   ...exist to control for these faults. However, it’s still unclear if similar pitfalls exist for CRISPR screens...

3. Sonic Hedgehog? Sleeping Beauty? Learn About the Genes Behind Addgene’s Conference Room Names

   Type

   Blog Post

   Published

   April 2, 2019, 1:44 p.m.

   ...is critical for processes such as cell movement/motility, development, cytokinesis, and muscle contractions...

4. Designing Your Chalk Talk for the Academic Job Interview

   Type

   Blog Post

   Published

   March 12, 2019, 1:37 p.m.

   ...laundry list of molecules to screen has unclear utility. You cannot predict what will happen. Without the...

5. Supporting Reproducibility with a Connected ELN

   Type

   Blog Post

   Published

   Jan. 17, 2019, 1:38 p.m.

   ...well as the DSpace repository. These integrations utilize an interface that enables users to conveniently...

6. CRISPR-mediated Plant Base Editors

   Type

   Blog Post

   Published

   Jan. 3, 2019, 1:35 p.m.

   ...be applied to fix the defective base precisely? Until 2016, precise single base changes were only possible...

7. CRISPR 101: Targeting RNA with Cas13a (C2c2)

   Type

   Blog Post

   Published

   Sept. 21, 2017, 2:07 p.m.

   ... processing in live cells. Indeed, groups have utilized dCas13 fusion proteins for imaging, tracking, ...

8. Deciphering the Mysteries of Behavior with Viral Vectors

   Type

   Blog Post

   Published

   Sept. 17, 2019, 12:40 p.m.

   ...Viral vectors, in contrast, are considerably more versatile and specific in how they manipulate neuronal function...

9. New Tools Enable CRISPRa for Neuroscience Applications

   Type

   Blog Post

   Published

   July 2, 2019, 12:51 p.m.

   ...CRISPRa? Although cDNA overexpression approaches may still be preferable for individual gene activation in ...

10. Viral Vectors 101: Preparing Pooled Libraries

    Type

    Blog Post

    Published

    July 13, 2023, 1:15 p.m.

    ...pre-made libraries aren’t quite plug-and-play: you’ll still need to titer and optimize before you can transduce...

11. How to Write a Scientific Review Article

    Type

    Blog Post

    Published

    Feb. 16, 2017, 3:30 p.m.

    ...continuity. Also don’t worry if you feel like you don’t still know enough about the topic. Just get as many words...

12. Using Video to Share Your Science: We Share Science

    Type

    Blog Post

    Published

    Nov. 3, 2016, 2:30 p.m.

    ...attention, though in reality much of our research still fails to reach our primary target audience – colleagues...

13. Changing Labor Laws Bring Increased Postdoc Wages

    Type

    Blog Post

    Published

    Oct. 25, 2016, 2:30 p.m.

    ...their salaries raised to the new level:  We are still waiting for an indication of what (most of) Harvard...

14. Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs

    Type

    Blog Post

    Published

    Oct. 27, 2015, 3 p.m.

    ... increase in fluorescent signal achieved when utilizing an optimized moxFP as opposed to standard FPs....

15. Plasmids 101: The Promoter Region – Let's Go!

    Type

    Blog Post

    Published

    April 3, 2014, 8:05 p.m.

    ...the cell, promoters themselves are controlled by still other regulatory factors: enhancers, boundary elements...

16. The Advances Behind The World's First Personalized CRISPR Treatment

    Type

    Blog Post

    Published

    Sept. 16, 2025, 1:15 p.m.

    ... in different patients. While these gRNAs would still need to be optimized and assessed for off-target...

17. Hot Plasmids: Summer 2024

    Type

    Blog Post

    Published

    Aug. 27, 2024, 1:15 p.m.

    ...overcome with methods such as chemical crosslinking or tilted-stage imaging. A truncated form of a tardigrade...

18. Antibodies 101: Introduction to Gating in Flow Cytometry

    Type

    Blog Post

    Published

    April 4, 2024, 1:15 p.m.

    ...following gates, cells that are not of interest can still be gated out later. Based on personal preference...

19. Adeno-associated Viruses (AAVs) for Genome Editing

    Type

    Blog Post

    Published

    March 27, 2018, 1:32 p.m.

    ...Additional Resources on the Addgene Blog AAV: A Versatile Tool for Gene Expression in Mammals AAV Titers...

20. Transferable Skills Guide: Conflict Resolution

    Type

    Blog Post

    Published

    Jan. 4, 2022, 2:15 p.m.

    ...customer support, but there are eight other teams who still retain a customer support element amongst their ...