We narrowed to 546 results for: TIL;
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TypeBlog PostPublishedApril 16, 2020, 1 p.m....bottleneck. In the last few years, scientists have utilized CRISPR-Cas9 protein variants, Cas13, and Cas12a...
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New Tools Enable CRISPRa for Neuroscience Applications
TypeBlog PostPublishedJuly 2, 2019, 12:51 p.m....CRISPRa? Although cDNA overexpression approaches may still be preferable for individual gene activation in ... -
Sonic Hedgehog? Sleeping Beauty? Learn About the Genes Behind Addgene’s Conference Room Names
TypeBlog PostPublishedApril 2, 2019, 1:44 p.m....is critical for processes such as cell movement/motility, development, cytokinesis, and muscle contractions... -
Designing Your Chalk Talk for the Academic Job Interview
TypeBlog PostPublishedMarch 12, 2019, 1:37 p.m....laundry list of molecules to screen has unclear utility. You cannot predict what will happen. Without the... -
Supporting Reproducibility with a Connected ELN
TypeBlog PostPublishedJan. 17, 2019, 1:38 p.m....well as the DSpace repository. These integrations utilize an interface that enables users to conveniently... -
CRISPR-mediated Plant Base Editors
TypeBlog PostPublishedJan. 3, 2019, 1:35 p.m....be applied to fix the defective base precisely? Until 2016, precise single base changes were only possible... -
Deciphering the Mysteries of Behavior with Viral Vectors
TypeBlog PostPublishedSept. 17, 2019, 12:40 p.m....Viral vectors, in contrast, are considerably more versatile and specific in how they manipulate neuronal function... -
Bacterial Toxin-antitoxin Systems as Molecular Biology Tools
TypeBlog PostPublishedNov. 1, 2018, 12:35 p.m....plasmid due to improper plasmid partitioning, it will still inherit some toxin and antitoxin. However, the antitoxin... -
Plasmids 101: Codon usage bias
TypeBlog PostPublishedSept. 27, 2018, 1:09 p.m....in the past (Angov et al., 2008), but there are still many more features to consider when designing a ... -
Controlling for Off-target Effects with a New Genome-wide CRISPR Screen Design
TypeBlog PostPublishedSept. 13, 2018, 1:55 p.m....exist to control for these faults. However, it’s still unclear if similar pitfalls exist for CRISPR screens... -
CRISPR 101: Targeting RNA with Cas13a (C2c2)
TypeBlog PostPublishedSept. 21, 2017, 2:07 p.m.... processing in live cells. Indeed, groups have utilized dCas13 fusion proteins for imaging, tracking, ... -
Using Video to Share Your Science: We Share Science
TypeBlog PostPublishedNov. 3, 2016, 2:30 p.m....attention, though in reality much of our research still fails to reach our primary target audience – colleagues... -
Changing Labor Laws Bring Increased Postdoc Wages
TypeBlog PostPublishedOct. 25, 2016, 2:30 p.m....their salaries raised to the new level: We are still waiting for an indication of what (most of) Harvard... -
Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs
TypeBlog PostPublishedOct. 27, 2015, 3 p.m.... increase in fluorescent signal achieved when utilizing an optimized moxFP as opposed to standard FPs.... -
Plasmids 101: The Promoter Region – Let's Go!
TypeBlog PostPublishedApril 3, 2014, 8:05 p.m....the cell, promoters themselves are controlled by still other regulatory factors: enhancers, boundary elements... -
The Advances Behind The World's First Personalized CRISPR Treatment
TypeBlog PostPublishedSept. 16, 2025, 1:15 p.m.... in different patients. While these gRNAs would still need to be optimized and assessed for off-target... -
Hot Plasmids: Summer 2024
TypeBlog PostPublishedAug. 27, 2024, 1:15 p.m....overcome with methods such as chemical crosslinking or tilted-stage imaging. A truncated form of a tardigrade... -
Antibodies 101: Introduction to Gating in Flow Cytometry
TypeBlog PostPublishedApril 4, 2024, 1:15 p.m....following gates, cells that are not of interest can still be gated out later. Based on personal preference... -
New Optogenetic Tools for Cytoskeleton and Membrane Control
TypeBlog PostPublishedSept. 28, 2023, 1:15 p.m....tools to control formation of lamellipodia and contractile stress fibers, and recently developed a full ... -
CRISPR 101: Homology Directed Repair
TypeBlog PostPublishedJan. 26, 2023, 2:15 p.m....will keep cutting and repairing your target DNA until a mutation is introduced disrupting one or both ...