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  1. Experimenting with New Careers while in Grad School

    Type
    Blog Post
    Published
    Feb. 25, 2016, 3:30 p.m.
    ...for CINSO. For instance, I tweeted to the Indiana State Museum and others when I was trying to find local...informal science educators, and ever since, the State Museum has been the best supporter and most productive...

  2. Light Sheet Fluorescence Microscopy

    Type
    Blog Post
    Published
    July 16, 2015, 2:30 p.m.
    ...reflected light by using a pinhole.  However, the excitation light excites all of the fluorophores as it passes...takes to image through a large z-stack (8).  One limitation of LSFMs using single side illumination, especially...

  3. Tips for Using BLAST to Verify Plasmids

    Type
    Blog Post
    Published
    May 29, 2014, 1:29 p.m.
    ...BLAST (blastx) search to check for expected ORFs, mutations or truncations. A primary advantage of blastx ...database. Another benefit is that a frameshift mutation present in the ORF is readily apparent when viewing...

  4. Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection

    Type
    Blog Post
    Published
    Dec. 12, 2013, 3:35 p.m.
    ...the central dogma. We have the beginnings of computational logic and data storage. What we don't have are...begin to imagine designing 10-gene systems with quantitative precision. There are few designers of biological...

  5. Simplify Cloning with in vivo Assembly

    Type
    Blog Post
    Published
    Oct. 18, 2018, 12:37 p.m.
    ...were more prohibitive at the time. With these limitations no longer an issue, bacterial in vivo cloning... N-terminus of a protein-coding sequence, or a mutation can be introduced in a gene, while adding a GFP-tag...

  6. INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

    Type
    Blog Post
    Published
    Dec. 7, 2020, 2:53 p.m.
    ...studies (e.g. using mariner), as a method of tagmentation for next-gen sequencing pipelines (e.g. using...the mini-transposon can occur in two different orientations, but is strongly biased towards T-RL, where ...

  7. Viral Vectors 101: The Retroviral Lifecycle

    Type
    Blog Post
    Published
    July 27, 2023, 1:15 p.m.
    ...Gene-Editing Systems Based on CRISPR/Cas: Current State and Perspectives. Viruses, 13(7), 1288. https://... the National Academy of Sciences of the United States of America, 54(1), 137–144. https://doi.org/10.1073...

  8. Deep Dive: Fixing and Permeabilizing for Immunofluorescence

    Type
    Blog Post
    Published
    Aug. 30, 2022, 1:15 p.m.
    ...Fixation allows you to freeze your samples in a given state or point in time. You want to halt degradative processes...methanol, or ethanol, work by dehydrating and precipitating cellular components. This method of fixation...

  10. CRISPR Antimicrobials

    Type
    Blog Post
    Published
    May 3, 2016, 2:30 p.m.
    ...the world is unprepared. Each year in the United States, two million people will be infected by antibiotic...defective phagemid; Bikard et al. did not observe any mutations at the target site. In a mouse skin colonization...

  11. Deciphering the Mysteries of Behavior with Viral Vectors

    Type
    Blog Post
    Published
    Sept. 17, 2019, 12:40 p.m.
    ...floating on the water surface. The latter reflects a state of behavioral despair, and the more time the animal...showed that the activity of a particular subset of excitatory neurons (Drd1) in mPFC is responsible for the...

  12. Save Time with Transient Plant Leaf Transformations

    Type
    Blog Post
    Published
    July 25, 2019, 12:03 p.m.
    ...cleaves a substrate, which will then form a blue precipitate. GUS is usually the first choice for establishing...transformation success. Luciferases are great quantitative reporters and use a substrate to produce bioluminescence...

  13. New Tools Enable CRISPRa for Neuroscience Applications

    Type
    Blog Post
    Published
    July 2, 2019, 12:51 p.m.
    ...calmodulin-dependent kinase II alpha (aCaMKII; excitatory neurons) and Synapsin-1 (pan-neuronal) promoters...cell types (microglia are a notable exception) necessitates such molecular gymnastics for sensitive applications...

  14. Plasmids 101: Codon usage bias

    Type
    Blog Post
    Published
    Sept. 27, 2018, 1:09 p.m.
    ...structures in 10 closely related yeast strains. Adaptation to changing conditions - Organisms often need...sometimes (but not always) be noted in in the “mutation” field on the plasmid page (see plasmid 87904 ...

  16. Transferable Skills Guide: Creativity

    Type
    Blog Post
    Published
    Jan. 18, 2022, 2:15 p.m.
    ...: creativity. I’ve heard quite a few scientists state that they’re not creative, usually saying that they...your break can be as simple as a few minutes of meditation or a walk to the break room for a sip of water...

  17. CRISPR-based Genome Editing Systems For Editing in E. coli

    Type
    Blog Post
    Published
    April 13, 2021, 1:15 p.m.
    ... E. coli B strains, 1 E. coli W strain, and 1 Tatumella citrea, with a total of 70 sites edited. We optimized...strain library with different gene dosages to facilitate swift optimization of strains. Multiple induction...

  18. Antibodies 101: Reading a Flow Plot

    Type
    Blog Post
    Published
    Feb. 6, 2024, 3:13 p.m.
    ...amount of marker that is present, which would be annotated like so: CD14high or CD14low.  Don’t be intimated...liver and kidney recipients. World Journal of Transplantation, 8, 23–37. https://doi.org/10.5500/wjt.v8.i1.23...

  19. 5 Reasons to Use Reddit for Science Communication

    Type
    Blog Post
    Published
    Oct. 20, 2016, 2:30 p.m.
    ...definitely glad we did - you can see some of the stats from the AMA to the right - the Reddit community...r/Science has a great team of moderators who facilitated scheduling, filtered out inflammatory questions...

  20. Getting the Most from Your Lentiviral Transduction

    Type
    Blog Post
    Published
    April 7, 2016, 2:30 p.m.
    ...storage may be a bit dubious due to lack of documentation and high turn-over of lab personnel. If you ...These binding events can be impaired by the electrostatic repulsion of the negatively charged cell with...
Showing: 501 - 520 of 705 results