Plasmid Cloning by PCR
Type
Blog Post
Published
March 29, 2016, 2:30 p.m.
... Various Addgenies...
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Another Pathway into Cells: iTOP
TypeBlog PostPublishedJune 23, 2015, 8:37 p.m....in a series of primary cell types using Cre and various loxP reporters. In both mouse and human embryonic... -
Mentoring for Scientists: I Have a Mentor, What Now?
TypeBlog PostPublishedMarch 6, 2014, 7:12 p.m....learning. Listen to our podcast interview with Harvard Medical School researcher Connie Cepko to learn... -
Interview: Ed Boyden on Optogenetics, Neuroscience, and the Future of Neuroengineering
TypeBlog PostPublishedMarch 4, 2014, 4:49 p.m.... other conditions. Addgene: You've contributed various plasmids to Addgene over the years, many of them... -
Reagent Repositories Are Speeding up Science During the Pandemic
TypeBlog PostPublishedAug. 4, 2020, 1:15 p.m....ATCC) is a private, nonprofit repository for a variety of materials for life science research, including... -
Scientists Map the SARS-CoV-2-Human Interaction Network
TypeBlog PostPublishedApril 9, 2020, 1:15 p.m....against SARS-CoV-2. As we’ve all been affected in various ways by the pandemic, it is clear that we have ... -
25 Work From Home Tips for Scientists
TypeBlog PostPublishedMarch 19, 2020, 1:15 p.m....Receive and provide support while being safe. @GHP_HarvardChan — Shekhar Saxena (@ssaxenageneva) March 16,... -
The Importance of High Titer for AAV Transductions
TypeBlog PostPublishedMarch 1, 2018, 2:11 p.m....the surrounding media. Nonetheless, there are a variety of ways you can optimize AAV production. We’ve ... -
Hot Plasmids - December 2019 - CRISPR pooled library, LipoGlo, CRISPRa, and Voltage Imaging Tools
TypeBlog PostPublishedDec. 10, 2019, 1:58 p.m.... Various Addgenies... -
Cultivating Community Science at BosLab
TypeBlog PostPublishedNov. 2, 2017, 1:35 p.m.... will involve getting the community to collect various soil samples, extract DNA, sequence it, and analyze... -
Plasmids 101: NGS Quality Control for Pooled Libraries
TypeBlog PostPublishedOct. 26, 2017, 1:59 p.m....optimized to maintain library diversity. If the various plasmids in the library are differently sized, ... -
How Dry Ice Affects Viral Vector Sample pH & How to Avoid It
TypeBlog PostPublishedJune 20, 2017, 2:30 p.m....then moved the tubes back to a -80°C freezer for various recovery periods between 0 and 96 hours. We compared... -
Improving Transduction Efficiency with Exosome AAVs
TypeBlog PostPublishedMarch 14, 2019, 1:54 p.m....colleagues from Massachusetts General Hospital and Harvard Medical School took a closer look at the AAV vectors... -
The MTA at Addgene
TypeBlog PostPublishedJuly 23, 2024, 2:17 p.m.... UBMTA. Because biological materials are widely varied with many properties, having well-understood standardized... -
Science Career Options
TypeBlog PostPublishedFeb. 23, 2017, 1:30 p.m....independently. Practical outdoor skills are needed in a variety of roles, including: Livestock Breeder Scientific... -
Plasmids 101: How to Verify Your Plasmid Using a Restriction Digest Analysis
TypeBlog PostPublishedAug. 28, 2014, 3:34 p.m....This method is time-intensive, so we recommend a variety of ways to screen and verify your plasmids. Here... -
Why and how to start studying chromatin (with Addgene's help)
TypeBlog PostPublishedJune 12, 2018, 11:40 a.m....that histone genes frequently have many different variant names, not all of which can be listed here. If ... -
Antibodies 101: ChIP
TypeBlog PostPublishedMarch 10, 2022, 3:25 p.m....DNA can then be isolated and studied through a variety of downstream methods, such as qPCR or next-gen... -
Developing a cold tolerant E. coli using specialized chaperones
TypeBlog PostPublishedJan. 28, 2020, 2:35 p.m....science, our team has continuously engaged with a variety of stakeholders from industrial and commercial ... -
Cas13d: Small RNA-targeting CRISPR enzymes for transcriptome engineering
TypeBlog PostPublishedMay 3, 2018, 1:48 p.m....displayed zero. Unlike Cas9, for which efficiency varies widely across guide RNAs, each guide tested with...