We narrowed to 846 results for: ins

...In the third installment in our podcast series, we chat with new Addgene Board Member, Michael Koeris... Koeris did his graduate work in Professor Jim Collins' lab (then at Boston University, now at MIT) where...Professor Timothy Lu (a graduate student in the Collins' lab at the time) got the idea to engineer bacteriophage...1:25 - 6:19 : Dr. Koeris' graduate work in the Collins' lab 6:19 - 8:26 : Dr. Koeris' reasons for wanting...

...Transformation grade DNA  Transformation is the process of inserting exogenous DNA in a microorganism. The amount of...cloning includes a set of techniques that are used to insert recombinant DNA from prokaryotes or eukaryotes ...from other nucleic acids (genomic DNA and RNA), proteins and chemical contaminants that have been used ... grade DNA. Transfection is the process of inserting an external plasmid in mammalian cells and it ...

... accomplished by using, for example, red light instead of blue to excite a molecule of GFP. Because red...a host of tools and opened doors to many great insights, it can be overwhelming to a novice microscopist...from a well-established microscopist at your institution when heading into a new set of experiments. ...Lightsheet Microscopy Choosing Your Fluorescent Proteins for Multi-Color Imaging Choose the Brightest Fluorescent... fluorescent proteins...

...wider community of users.  We also made scientific gains by adding long-read sequencing capabilities to complement...quality control. Through our partnership with the Institute for Protein Innovation (IPI), we expanded a growing...contributions to key research papers are already inspiring higher standards in antibody use (Ayoubi et al...R. A., Virk, H., Laflamme, C., Houston, D. W., Polinski, N. K., Meijers, R., Levey, A. I., Saper, C. B...

...Technique: Probe Phage Genomes for Host Binding Proteins & Running for Rare Disease, Running for FOP, Running...Technique: Probe Phage Genomes for Host Binding Proteins," was contributed by guest blogger Jessica Sacher...describes a technique you can use to find phage proteins that bind to your bacterium of interest. For those...an ingenious but simple technique to screen for proteins that could be useful for a wide variety of reasons...

... suffer any less acutely.  The Rare Genomics Institute works to help those who suffer from rare diseases...Through their BeHEARD Award, the Rare Genomics Institute partners with companies to provide technological...Elia Di Schiavi is a Research Scientist at the Institute of Biosciences and Bioresources in Naples, Italy...interactions with other genes. He will use fluorescent proteins to monitor the localization of these genes, CRISPR...

...capsid proteins that determine the AAV serotype. During transduction, the viral capsid proteins bind to...Education and Development workshop aimed at brainstorming strategies for using combinations of AAV-based... to the target cell’s surface proteins and this interaction determines the specificity and efficiency ...

...evaluate the impact of their work (H-index for instance), but these rely upon other researchers being ...plasmids that can be used for specific purposes. For instance, on our Optogenetics Collection page, you can ...containing plasmids for expressing different types of rhodopsins and sensors as well as references and links to... Blog Post on Our Citations Feature Browse More Inside Addgene Posts Additional Resources on Addgene....

...the presence of scientific heroines is always inspiring. I was especially excited to attend this event...genome. Their award acceptance addresses were inspiring! We also got to hear more from them during a panel...especially thanked Martin Jinek and Krzysztof Chylinski (who coincidentally speak the same Polish sub-...to generate mice with genes knocked out in the insulin signaling cascades in only 4 months compared to...

...does increase the chance of false-positives, as proteins may appear to be binding at particular genomic...Nuclei are then incubated with a primary antibody against the protein of interest. The Protein A fused MNase...G (IgG) thus targeting MNase to antibody bound proteins (Figure 1). Importantly, since Protein A has weaker...interactions Learn about other types of fusion proteins Study chromatin interactions with CAPTURE Additional...

...recycled. This service does cost money, but your institute may still be interested as it offsets the cost...how to start a glove recycling program at your institute! What should I do in the lab to help the environment...back. Start a glove recycling program at your institute! Universities and companies have the potential...posts and updates from Labconscious. If you’re inspired by this post then I challenge you to take to action...

...Microbiology (ASM) Scientific Writing and Publishing Institute. Writing is the cornerstone of any scientist’s...and the ASM Scientific Writing and Publishing Institute (SWPI) is an extremely beneficial guide to becoming...Di Rita, and, last, homework assignments. The instructors are leaders in the field of microbiology with...writing everyday Overall, the most important instruction the classes taught me was to write every day....

...and Leaders. I like her use of the term “cycles” instead of levels because it emphasizes the importance ...started and then step back to let others take the reins. They work alone much of the time, depending on ...it is the leader’s job to provide the driving inspiration, to create a productive culture and to correct... by Peter Fuda and Richard Badham Leadership Insights for Engineers, Scientists and Computer Professionals...

...molecular biology to isolate, multiply, or express the insert they carry in the target cell. These vectors allow...purify it – and you do not have a specific antibody against the protein available – you can always add an epitope...mammalian expression, a multiple cloning site to insert YGOI, a bacterial selection cassette for growth... collection of vectors carrying several fusion proteins. Pick your promoter, selection cassette and visualization...

...of the Ocular Genomics Institute, and an Associate Member at the Broad Institute of Harvard and MIT. ...In 11th grade she heard a talk about Z DNA that inspired her to major in molecular biology in college. ... of Oncology Target Discovery at the Novartis Institutes for Biomedical Research. Drug development was...

...communication graduates are often well-equipped. Many institutions are sincerely interested in connecting with ...the experimental aspect of my thesis: a survey instrument designed to measure how individuals respond to...science. Understanding just how the public utilizes insights gleaned from scientific research is, after all... this power and the tools to deploy its wisdom remains a challenge. Departments like ours reflect a growing...

...have recently received a grant from the National Institutes of Health that will address this problem.The ...produce a subset of this collection as ready-to-use-proteins to facilitate use. Dr. Trimmer’s lab will ensure... represent the official views of the National Institutes of Health.     References and Resources References...10.1038/520295b Bradbury ARM, Trinklein ND, Thie H, Wilkinson IC, Tandon AK, Anderson S, Bladen CL, Jones B,...

...locus or more commonly via cloning of recombinant proteins. Why use a tag? If you are fortunate enough to...may not need to read this - but for many other proteins, quality antibodies do not exist, or they don’...benefits – as discussed above – but there are a few instances when they should be used with caution or not at...protein may disrupt the biological function of some proteins. Protein folding issues, steric hindrances, and...

... use similar processes to add overhangs to your insert of interest for Gibson assembly. The steps following...to choose enzymes that: Do not cut within your insert Are in the desired location in your recipient...our cDNA into the recipient plasmid. Remember to insert your DNA in the correct orientation in the recipient...cloning Run the PCR reaction Run PCR to amplify your insert DNA. It is important to use a high fidelity polymerase...

...secondary structure that can bind specific proteins via aptamer domains. Combined with dCas9, this new scaffold... sites. With hundreds of characterized aptamer domains available, an unprecedented level of flexible tunability... Designing and using RNA scaffolds to assemble proteins in vivo. Nature protocols. 7(10) 1797-1807 (2012...