We narrowed to 823 results for: lars;

...plasmids at Addgene Read our molecular biology reference Watch molecular biology protocol videos ...that have lost the sacB gene. SacB can be used similarly to CcdB. For example, in the gene expression plasmid...

...Anyone who works in a lab is familiar with the alarming amount of waste that is generated. This is a systemic...first post will be based on recycling! With the popularity of single-use items, plastic is a substantial... still be interested as it offsets the cost of regular waste services. Learn about how to start a glove...

...for human c-myc proto-oncogene product. Molecular and Cellular Biology, 5(12), 3610–3616. doi: https:/... and two-photon excitation around 1,040 nm, has larger photocurrents, and is more selective for K+ than...

...Robertson (far left) is a Senior Lecturer and a Plant Molecular Biology and Biotechnology expert. In order to ...engineer plants that are able to destroy DNA viruses, similar to the anti-phage CRISPR effect observed in bacteria...Nair, Associate Professor (Right), focuses on molecular biotechnology for improving expression of nutritionally...

... events are one of the main ways budding young scholars across Europe get introduced to the cultures and...collaborations and conferences for their students. Another large goal of the organisation is to encourage iGEM alumni...these biological societies engaging with young scholars. We believe it’s very important to develop a network...

...welcoming us. Then it was time to work! Set up similarly to the online class, we met as a group for sessions...colleagues’ manuscripts and discuss changes to particular sections. The focus groups allowed us to try ...try out techniques that were discussed in the larger sessions and reinforced our newfound knowledge with...

...but for viral vector core facilities producing a large number of viral preparations in parallel, there ...Single-stranded Virus Next-generation Sequencing. Molecular Therapy - Nucleic Acids 4:e260 . https://doi.org...Batch-to-Batch Consistency Evaluation of AAV Preparations. Molecular Therapy 25:1375–1386 . https://doi.org/10.1016...

...interested in exploring biological dark matter – large tracts of the microbial tree of life that remained...IgnaviCas9 is a type II-C Cas9. IgnaviCas9 is most similar to a type II-C Cas9 from Parvibaculum lavamentivorans... incredible thermostability makes the enzyme particularly useful. Additional resources on the Addgene...

...that cells with common ancestors tended to have similar reprogramming outcomes. For example, cells with...with shared CellTagD13 tags often had similar gene expression pattern changes and reprogramming outcomes...reprogramming is not stochastic, but rather there’s a particular cell state that’s permissive to reprogramming.In...

... including more secure archiving, unambiguous molecular definition, and potential for further engineering...is a critical unmet need for an open-access, molecularly defined, and well validated collection. I’m thrilled..., Wong J, Koh XY, Eng H-Y, Lane D, Lefranc M-P, Clark M, Dübel S (2018) When monoclonal antibodies are...

...low concentration or you are cloning an extremely large insert. Since the standard ligation reaction is...Central PMCID: PMC52876. 2. Novel approach to molecular cloning and polynucleotide synthesis using vaccinia...Plasmid Cloning Protocols Catch Up On Your Basic Molecular Biology Techniques Watch Our Protocol Videos ...

...problem, Gao et al. searched for Argonaute orthologs similar to TtAgo and PfAgo, another thermophilic Argonaute...variety of mammalian cell lines, with an efficiency similar to that of Cas9. Like Cas9, NgAgo is also able ...cleavage efficiency. If this finding is replicated in larger studies, it will represent a key advantage over...

...undeniably difficult connection to study on a molecular level. The brain is constantly influenced by signals...organisms, including the Anikeeva lab’s work on a similar neural fiber (Park et al., 2019). This provided...., Anikeeva, P., & Chandrakasan, A. P. (2020). Modular Optoelectronic System for Wireless, Programmable...

...of Addgene's top blog posts from 2015 Our most popular posts continue to be those from our Plasmids 101...regardless of their end goals and believe the popularity of our career posts shows that we are doing just...Ramsden from UNC Chapel Hill explain some of the molecular gymnastics behind DNA repair by non-homologous...

... Syndrome One of those teams is led by Claudio Aguilar (right) at Purdue University and is working to ...ago, but there is still no treatment available. Aguilar is a basic cell biologist. He got interested in...meet a few patients that suffered this disease,” Aguilar said. “Once you have that, there is no coming back...

...individuals in their 20’s who’ve built multibillion dollar tech companies using only laptops and some server...story altogether? The unfortunate answer is yes, largely due to a few limiting factors.  To start, the average...different than what was originally planned, due in large part to the feedback we received from our network...

...eight hours worth of hands-on time. Creating a modular, selection-based gene tagging approach Multiple...promoter-FP fusion for the gene of interest. L1/L2 larvae from these strains are then heat shocked to remove... this system Dickinson et al. have developed a modular system that requires very little hands-on labor...

...being used to study SARS-CoV-2. The resurgence in popularity of the hACE2 transgenic mouse strain, for example...murine ACE2 (Subbarao et al., 2006). Now that a similar phenomena is appearing with regards to SARS-CoV...SARS-CoV-2, this mouse strain is regaining popularity. The Jackson Laboratory, a nonprofit research institution...

...problem can be attributed to communication, and in particular communication about experimental methods and ... and funding, so institutions and funders, to a large extent, hold the keys to getting transparency and...journals including Trends in Cell Biology, Cell, Molecular Cell, Developmental Cell, and Cell Stem Cell.  ...

...insects, mammals, and plants, among others. I particularly enjoyed the emphasis that microbes are neither... "The Mistborn series is the first 3 books of a larger epic of fantasy novels. Set in a world where 1000...Control Jonathan Strange & Mr. Norrell by Susanna Clarke "A quasi-historical/fantasy fiction. Two dueling...