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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 714 results for: met

Showing: 501 - 520 of 714 results

  1. Viral Vectors 101: An Introduction to AAV

    Type
    Blog Post
    Published
    May 30, 2023, 1:15 p.m.
    ...the both ends and mutated ITR at the center of symmetry. After uncoating in the target cell nucleus, this...optimization of a real-time quantitative PCR-based method for the titration of AAV-2 vector stocks."Molecular...

  2. Choosing the B(right)est Fluorescent Protein: Photostability

    Type
    Blog Post
    Published
    June 8, 2017, 2:30 p.m.
    ...al, 2017) and cannot be described by a single parameter. Therefore, it is necessary to know how the fluorescence... (iii) attached to a substrate. These in vitro methods allow for a well-controlled environment, but they...

  3. Transferable Skills Guide: Cross-team Communication

    Type
    Blog Post
    Published
    Nov. 21, 2019, 1:53 p.m.
    ... a month or so of meetings, I began to notice something: even though our scientists and engineers were...likely will need to write protocols, SOPs, and methods sections. The next time you write or read one of...

  4. Developing Lab Management Software for Biology

    Type
    Blog Post
    Published
    Aug. 25, 2016, 2:30 p.m.
    ...are no explicit instructions on what to do when something fails. While we’re sure our techs could figure...the contents of a tube with the tube itself. If something happens to the worm that requires a change of ...

  5. Addgene's Tips for Plasmid Quality Control

    Type
    Blog Post
    Published
    Jan. 14, 2016, 3:30 p.m.
    ...incoming plasmids are sequenced at least twice, and sometimes more depending on the number of features we need...sequencing reactions. In the more complex cases, we sometimes have to design new primers to sequence the important...

  8. CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9

    Type
    Blog Post
    Published
    Nov. 14, 2024, 2:15 p.m.
    ...target ncRNA. CRISPR/Cas9 techniques and targeting methods Knock-out: Targeting short vs. long ncRNAs The ...transcription of most of the ncRNA (Figure 1D). While this method can be time-consuming, it is relatively effective...

  10. Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene

    Type
    Blog Post
    Published
    April 6, 2021, 12:30 p.m.
    ...will update information on the plasmid’s page. Sometimes we’re missing a piece of the plasmid There are...make available as part of our OpenBio Repository sometime soon. Stay posted!     References Guerin K, ...

  11. Antibodies 101: Choosing the Right Isotype

    Type
    Blog Post
    Published
    April 6, 2023, 3 p.m.
    .... L., & Partridge, T. A. (1998). A New Blocking Method for Application of Murine Monoclonal Antibody to...Techniques for Light and Electron Microscopy. In Methods in Cell Biology (pp. 283–310). Elsevier. https:...

  12. Plasmids for Endogenous Gene Tagging in Human Cells

    Type
    Blog Post
    Published
    April 6, 2017, 1:02 p.m.
    ... elucidate cellular structures. Overexpression methods flood the cell with protein, which can both interfere...with BioRender.com. A key feature of our methodology is the use of a donor plasmid that contains long...

  13. 6 Tips for Grant Writing

    Type
    Blog Post
    Published
    Oct. 18, 2016, 2:30 p.m.
    ...these findings will be worth knowing? This can sometimes be tricky, as most of our research builds on ideas... successful applications, I can't always find something that made them better than my unsuccessful ones...

  14. Tips for Technical Support Calls

    Type
    Blog Post
    Published
    Sept. 20, 2016, 2:39 p.m.
    ...answer several emails that start: “Hi! We ordered something from you and it’s wrong. Can you help us fix it...a very positive experience, also let us know! Sometimes those few “thank you”s are what get a customer...

  15. Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015

    Type
    Blog Post
    Published
    Dec. 1, 2015, 3:30 p.m.
    ...transcripts. I remember when small RNA was just something we ran off the bottom of the gel so it wouldn’...thought. High throughput transcriptome sequencing methods including work done by the ENCODE project consortium...

  16. Experimenting in an Open Source lab: from CRISPR to Cats

    Type
    Blog Post
    Published
    July 23, 2015, 2:30 p.m.
    ...bioinformatics pipeline. It’s possible that we’ll find something interesting and contribute it back to the kittybiome...printer, a laser cutter, woodworking tools, and a metalshop. After two summers in Silicon Valley as a student...

  17. Story of a SynBio Startup: RevBio's Epiphany (or Lack Thereof)

    Type
    Blog Post
    Published
    June 5, 2014, 3:32 p.m.
    ...on any challenge and quickly become experts in something, even if that thing is opening a pizza joint. ...analyze what we want out of our careers: to build something new and beautiful, to engage with the public in...

  19. Plasmids 101: Protein Expression

    Type
    Blog Post
    Published
    June 7, 2018, 1:17 p.m.
    ...from contaminants using a variety of purification methods. Deciding which expression system to use depends...transiently or through stable cell lines. Both methods produce high protein yields if transfection is ...

  20. The Scientific Conference Poster Session: Tips for Success

    Type
    Blog Post
    Published
    Aug. 29, 2019, 12:50 p.m.
    ...down the question you were trying to answer, the method you used, the main result, and the fundamental ...you’ve already set yourself up for success. But sometimes, attendees can get sucked in to thirty-minute-...
Showing: 501 - 520 of 714 results