...students focus on the application process, and simplify access to academic resources, we created STEAMid...worried about any eligibility provision, don’t guess. Simply reach out to the program coordinator with your ...Chemical Biology Program at Harvard. Roo wants to simplify access to academic resources among economically...

...reliable and reproducible. However, we do not use amplicons in ITR as we have found this to strongly bias ...somewhat more reliable quantitation. Differences in sample treatment across methods can affect reported titer...that users keep titering method consistent across samples within a project. Targeting Your AAV Q4: Are there...

...cool because they can transmit information, for example, from one of the 180 regions to another or from...the brain to the rest of the body. Therefore, by simply looking at how neurons are physically connected...even further defined population of neurons. For example, say I wanted to access all the neurons that signaled...

...applications, while electroporation of a human, for example, isn’t a reasonable option. Since viruses used...infect both non-dividing and dividing cells. They accomplish this through nuclear localization signals which... immunodeficiency virus type 1 preintegration complexes.” Proc. Natl. Acad. Sci. U.S.A. 89 (1992) 6580...

...comprised of 2 plasmids, pPSU1 and pPSU2, that can be amplified affordably in bacteria. They are compatible with...was also independent of another TTAGGG binding complex, shelterin. Swapping the DNA binding domain of ...that can be combined with your gRNA and repair templates of choice though modular cloning steps. The Voytas...that are sure to be enabled by this toolkit. For examples of the toolkit in action, see Cermak T, et al....

...show. After graduating with a master’s, Susan completed a science writing internship with the National...planning.” To beef up her resume, she decided to complete a certificate in Strategic Communications and ...the most satisfying parts of the job is having completed one of these difficult pieces: “It’s probably ...

...preserves RNA structure and naturally occurring complexes present in the cell, it can also introduce non-physiological...non-physiological RNA-protein interactions due to sample processing. Require prior knowledge that the protein...levels seen in HHCS patients. These are just three examples of how RaPID can be used to study RNA-protein ...

... practice to compare degradation rates between samples treated with or without an autophagy inhibitor....accumulates in the vacuole. See Figure 2 for an example of what fluorescence looks like for cytoplasmic...Fluorescent Protein Oligomerization Seeing Red: Simple GFP Photoconversion Light Sheet Fluorescence Microscopy...

...several different species are present. One common example is using pre-adsorbed secondary antibodies (which...antibodies are polyclonal, as their function is to amplify the signal from a primary antibody; the ability...target protein thereby greatly increasing signal amplification.  When to stay away from polyclonal antibodies...

...intensive to process a large number of microbiome samples. To enable large-scale, longitudinal studies of...and can approximate mRNA concentration within a sample. Using this system, the Collins lab was able to...lab validated their platform on clinical stool samples by comparison to qPCR and demonstrated that it ...

...necessary” detail after “necessary” detail and end up completely overwhelming your audience - like the professor...intuitive that actually wasn't. Maybe I needed to employ a visual aid instead of describing something verbally...group was just really tired and needed a slower, simpler presentation than I had planned; other times students...

... the LoxP sites, which is more difficult than a simple knock out. Looking for some permanent knock out...two varieties: tet-on and tet-off. As the names imply, tet-off turns off constitutively expressed proteins... tunable control These systems can be easily implemented for exogenously introduced proteins, as many ...

...the take rate of mouse xenografts after cell implantation. Their goal was to figure out how to optimize...barcodes in the population (all barcodes can be amplified using the same sets of forward and reverse primers...dynamics in response to cancer therapy using high-complexity barcoding." Nature medicine 21.5 (2015): 440-...

... the xanthine produced via complete demethylation of caffeine. For example, the Barrick lab was able to...living biosensor for measuring caffeine content in samples. The Barrick lab and the UT Austin undergraduate...

...treatments, but are not useful for genetic engineering. Examples of outbred strains include Swiss Webster and Harlan... are injected into a blastocyst which is then implanted into a female animal for gestation. Nucleases ...are microinjected into a zygote, which is then implanted into a female mouse for gestation. All three can...

...animal models of disease and cultured cells. For example, in 2013, Jinsong Li and colleagues chose to explore...trials is somewhat similar (Hussein et al). It employs Cas9 to cut at two sites within the viral genome...current delivery technologies are suited for. For example, lipid nanoparticle delivery tends to be trafficked...

...In order to understand the complex wiring of the brain and the underpinnings of neurological disease,...while resulting in minimal off target effects. For example, if your receptors are expressed in the brain, ...receptor acts through. The DREADD hM3D(Gq), for example, lowers the threshold for neuronal signaling and...

...loxP, black arrowheads) and one mutated pair (for example- lox511, white arrowheads). It is necessary to ...express GeneR only in the presence of Cre. In this example, Cre would first invert the reporter and GeneR ...the presence of Cre. A similar strategy can be employed for "Cre-off" (i.e. your gene of interest starts...

...predecessors. Changes were implemented and I had to adapt accordingly. For example, the quarterly and annual...volunteered for various tasks as things came up. For example, I updated some sections of the NPA’s website. ...

... an enhanced pU6cm promoter, viral replicon amplification of cargo, and heat treatment.  Figure...detection of activation triggered by endogenous GPCR. Example applications include large-scale pharmacological...recombinant antibody in human postmortem brain samples. Results show positive staining in tissues from...