We narrowed to 723 results for: tat

... that was not “poised for cleavage”. Jacob computationally added the non-template strand to the Jinek ... this model also displays the several Lys/Ala mutations reported by Ian Slaymaker and colleagues to reduce...

..., tobacco, rice, sugarcane, and even tubers of potatoes have been used for protein expression. Plant ...well-defined media and can be easily adapted to fermentation allowing for large-scale, stable production ...

... Base editing, another method to create point mutations, also has a strict editing window about 15 nt ...mutagenesis plasmid (MP6) permits quick, large-scale mutation of the gene of interest. They created a selection...

...chance of a chromosomal inversion, or just random mutations if the chromosome is repaired with all the original...deleting a problematic copy of a gene that causes a debilitating disease! This is, of course, only hypothetical...

...eventually found a version of AsLOV2 with five mutations (C450P, L496V, Q513K, G528K, D540M) that shows... and ΔF/F of MagLOV, a variant of AsLOV2 with mutations C450P, L496V, Q513K, G528K, D540M, in response...

...However, many fluorescent proteins face similar limitations, and many labs are already using these chromoproteins...promoter. The resolution step leads to the desired mutation and excision of the vector backbone. To do this...

...partner; as I write this, there are close to 10,000 citations for anti-Myc 9E10 in CiteAb, many of which are...no antibody is that perfect. One of the known limitations was described by Schüchner, et al., who published...

...industry, you’re more likely to give an oral presentation to highlight any new data or ask for additional...industry with most team members giving oral presentations, you will also have to attend many more meetings...

...due to its endA1 recA1 relA1 genotype, as these mutations improve plasmid stability and yield. If a plasmid...using older plates could increase plasmid loss or mutation. Let it incubate Increasing the incubation time...

...for CINSO. For instance, I tweeted to the Indiana State Museum and others when I was trying to find local...informal science educators, and ever since, the State Museum has been the best supporter and most productive...

...reflected light by using a pinhole.  However, the excitation light excites all of the fluorophores as it passes...takes to image through a large z-stack (8).  One limitation of LSFMs using single side illumination, especially...

...BLAST (blastx) search to check for expected ORFs, mutations or truncations. A primary advantage of blastx ...database. Another benefit is that a frameshift mutation present in the ORF is readily apparent when viewing...

...the central dogma. We have the beginnings of computational logic and data storage. What we don't have are...begin to imagine designing 10-gene systems with quantitative precision. There are few designers of biological...

...Gene-Editing Systems Based on CRISPR/Cas: Current State and Perspectives. Viruses, 13(7), 1288. https://... the National Academy of Sciences of the United States of America, 54(1), 137–144. https://doi.org/10.1073...

...Fixation allows you to freeze your samples in a given state or point in time. You want to halt degradative processes...methanol, or ethanol, work by dehydrating and precipitating cellular components. This method of fixation...

...studies (e.g. using mariner), as a method of tagmentation for next-gen sequencing pipelines (e.g. using...the mini-transposon can occur in two different orientations, but is strongly biased towards T-RL, where ...

...were more prohibitive at the time. With these limitations no longer an issue, bacterial in vivo cloning... N-terminus of a protein-coding sequence, or a mutation can be introduced in a gene, while adding a GFP-tag...

...follows: (my_house + coffee) - starbucks See how the notation works? The + operator denotes intersection and...Through a bit of stochastic magic and Poisson statistics, each sub-population has, on average, one distinct...

...the world is unprepared. Each year in the United States, two million people will be infected by antibiotic...defective phagemid; Bikard et al. did not observe any mutations at the target site. In a mouse skin colonization...

...were designed to target genomic sites with no annotated function, i.e. sites that lack open chromatin ...safe-targeting guides, which target genomic sites with no annotated function. In the growth screen, safe-targeting...