...in just 2 years and excitement about how much more there is left to do with the characterization and implementation...relatively isolated resorts creates a sense of camaraderie with fellow attendees from the moment you arrive...motto for scientists who contact us and want a guarantee that their experiments will work (yes, this happens...

...interferon gamma (IFNγ) - and eliminate cancer cells. 2. Equilibrium - In a process analogous to the development... 35. Academic Press, 2015. PubMed PMID: 25818339. 2. Curiel, Tyler J., et al. "Specific recruitment of...thanks to our guest blogger Subhadra Jayaraman! Subhadra Jayaraman is currently a doctoral candidate at...post was contributed by guest blogger Subhadra Jayaraman, a doctoral candidate at Binghamton University... level of progression, anatomical location, characteristic (solid localized tumors or systemic tumors)...

...establishing the dynamic range of your FRET assay (Figure 2). These labeling methods are freely interchangeable...PMID: 20161833. PubMed Central PMCID: PMC2802762. 2. Dumas, Anaëlle, et al. "Designing logical codon reassignment–Expanding...), quantifying antibody/antigen interactions (Saraheimo et al., 2013), and visualizing structural changes...fluorophores, it’s best to consider these cases separately when talking about the tools that are available...tools for labeling reagents and structurally characterizing large ion channels involved in skeletal muscle...40.3 (2006): 279-286. PubMed PMID: 16908183. 10. Saraheimo, Satu, et al. "Time-Resolved FRET-Based Approach...

...from a single Illumina MiSeq® run. As shown in Fig. 2, below, differences in input concentration across ...many labs. However, the high costs of sample preparation for high-throughput next generation (NGS) sequencing...Addgene to develop and apply our plexWell™ Library Preparation Technology for NGS-based sequencing and confirmation...of barcoded fragments is known as a *library*. Parallel sequencing of the fragments within a library allows...understated problem in multiplex NGS library preparation. The challenge here is that, as more samples ...minimize these issues, researchers usually include a separate normalization step where individual input DNA ...

...PMID: 25386923. PubMed Central PMCID: PMC4227841. 2. Young, Carissa L., Zachary T. Britton, and Anne S...number of disulfide bonds in your protein - two parameters that affect how well E. coli can produce your...

...access this tool, set up an IDT account. Figure 2: Addgene's Analyze Sequence tool.   DNA sequence...complement sequence.  Filter DNA tool: Removes non-DNA characters, such as numbers and blank spaces, from the text... primer dimer estimation, and other primer characteristics. NEB Data-optimized Assembly Design (DAD): ...

...development and therapy. Nat Rev Drug Discov (2017) 16(2):89-100. DOI: 10.1038/nrd.2016.238 Additional resources...drugs. While we often think of these two fields as separate, they are deeply intertwined. In this blog we ...Target identification Not all diseases are fully characterized or have a simple pathology. In these cases, ...

...motion videos is very time consuming; producing a 1-2 minute video takes an entire week of our free time...gotten easier now that I identify with the main character in the video. We’ve grown attached to the work...“WOW, now I have my own laboratory” (the main character is much more than a piece of plastic for us now...

...incorporation of biosensors, protein evolution lineages, 2-photon spectra, more elaborate spectrum analysis tools...complicated entities with a large range of characteristics (color, brightness, photostability, maturation...attach important references that serve to further characterize a protein of interest (and we very much appreciate...simplifies the process of building advanced multi-parametric search queries.  Looking for a bright, monomeric...emission peak around 600nm?  Simply input these parameters and you’ll find matching FP suggestions. If ...

...posts: Part 1: Genetically Engineered Mice and Part 2: Breeding and Crossing Mice. My first CRISPR mouse...is less predictable. In our best case, we saw that 2 out of 3 mice carried a 5 kb insertion in the ROSA... a mouse model, and its success is not always guaranteed. To learn more about this process check out our...