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  1. Open Resources and Plasmid Tools For Studying C. elegans

    Type
    Blog Post
    Published
    July 18, 2019, 12:55 p.m.
    ...again reminded of the extent that C. elegans researchers embrace open science and share resources and ... what’s new and popular! References Hammarlund, Marc, et al. "The CeNGEN Project: The Complete Gene Expression...developmental dynamics database." Nucleic acids research 41.D1 (2012): D732-D737. PubMed PMID: 23172286...23172286. PubMed Central PMCID: PMC3531189. Mangone, Marco, et al. "UTRome. org: a platform for 3′ UTR biology...biology in C. elegans." Nucleic acids research 36.suppl_1 (2007): D57-D62. PubMed PMID: 17986455. PubMed ...
  2. CRISPR Activators: A Comparison Between dCas9-VP64, SAM, SunTag, VPR, and More!

    Type
    Blog Post
    Published
    Oct. 6, 2020, 1:15 p.m.
    ..., expanding its practicality and popularity in research (Tanenbaum et al., 2014, Konermann et al., 2015...the most popular methods for CRISPR activation, research in the area has continued to develop. These newer...an engineered CRISPR-Cas system. Nucleic Acids Research 41:7429–7437 . https://doi.org/10.1093/nar/gkt520...Brigham MD, Trevino AE, Joung J, Abudayyeh OO, Barcena C, Hsu PD, Habib N, Gootenberg JS, Nishimasu H,...Addgene's CRISPR Guide Find CRISPR plasmids for your research ...
  3. Viral Vectors 101: Chemogenetics

    Type
    Blog Post
    Published
    July 11, 2023, 1:15 p.m.
    ...Find viral preps for your research Find chemogenetics plasmids for your research ...Jendryka et al., 2019), they have resulted in a search for alternative ligands, such as C21, CLZ, Perlapine.../10.1021/cn500325v Goutaudier, R., Coizet, V., Carcenac, C., & Carnicella, S. (2019). DREADDs: The Power...Sternson S (2019) Ultrapotent chemogenetics for research and potential clinical applications. Science 6436...
  4. A Guide to Designing a Scientific Poster: Content, Layout, and More

    Type
    Blog Post
    Published
    Oct. 22, 2019, 12:20 p.m.
    ...You’ve done great research and made interesting discoveries. You’ve analyzed the data and generated beautiful...generally contains the same sections as a primary research article, it’s important to understand that presenting...is more! Posters should not look like a primary research article condensed into one big page. Your sections...Michigan State University. The idea is that the main research finding is written across the center of the poster...figures flank the central region, and a scannable barcode is included that links readers to more information...
  5. 5 Tips for Troubleshooting Viral Transductions

    Type
    Blog Post
    Published
    Aug. 11, 2016, 2:23 p.m.
    ... technical challenges they can present. Many researchers find themselves plagued by troubleshooting a ...sensitive to freeze thaw cycles. Depending on the researcher and the type of virus, there have been varied...virus will be used in a couple of days, some researchers prefer to store freshly harvested virus at 4 ...transport and delivery." Japanese journal of cancer research 93.5 (2002): 598-603. PubMed PMID: 12036457. 6...transfected DNA into normal human cells."Nucleic acids research 18.9 (1990): 2733-2738. PubMed PMID: 2339059. ...
  6. AAV Vector Quality Control: Going the Extra Mile with NGS

    Type
    Blog Post
    Published
    Sept. 12, 2017, 1:44 p.m.
    ...continue our mission of accelerating scientific research. To make sure that adeno-associated viral (AAV...impurities are generally considered innocuous for research-grade vectors, we’d like to make sure they are...reference sequence. Second, we perform a megaBLAST search on all the reads that did not map to the reference...clinical-grade materials, and is not commonly performed for research-grade materials. Addgene is going the extra mile...that you receive the best AAV vectors for your research. Please use the comments section below to let ...
  7. Starter guide to induced pluripotent stem cells (iPSCs) part 1:  A renaissance in regenerative medicine

    Type
    Blog Post
    Published
    Oct. 30, 2018, 1:04 p.m.
    ...fertilization treatments and donated afterwards for ESC research purposes, with the informed consent of the donor...and adult stem cells can be used for different research and therapeutic purposes. Both types have their...skirts issues surrounding the use of embryos in research. With all these benefits, iPSCs have increasingly...increasingly become the ideal experimental system for research in regenerative medicine over the last decade....Richards, History and perspective of stem cell research. Best Pract Res Clin Obstet Gynaecol, 2004. 18...
  8. Delivery Methods for Generating iPSCs

    Type
    Blog Post
    Published
    April 17, 2018, 1:37 p.m.
    ...a strategy suited to their research. Find Plasmids for Stem Cell Research Delivery Method Mechanism...layers. Choosing a reprogramming method Most iPSC research falls into two categories: 1) studies focused ...at least one method that can help you with your research goals. Are you a new or experienced reprogrammer...The promise of induced pluripotent stem cells in research and therapy." Nature 481, no. 7381 (2012): 295... Cells Check Out Our Interview with Stem Cell Researcher Darrell Kotton Four Factors that Differentiate...
  9. An Introduction to Adenovirus

    Type
    Blog Post
    Published
    Oct. 3, 2023, 1:15 p.m.
    ...between early and late gene transcription) is demarcated by viral genome replication (Harrach, 2014). ... majority of adenoviral vector systems used in research, they are only expressed in the producer cells... to standard uses in gene therapy and in basic research, like other viral vectors, though significant ...inspires further use of existing vectors, as well as research into the development of new adenoviral vectors...., Bazinet, A. L., Bergman, N. H., Bull, R. L., Garcia, K. Y., Goodrich, J. S., Lovett, S. P., Parker,...
  10. Viral Vectors 101: The Retroviral Lifecycle

    Type
    Blog Post
    Published
    July 27, 2023, 1:15 p.m.
    ...non-living viral particle, and how we as dedicated researchers can exploit it. This blog was written by Addgenie...10.3390/v13071288. PMID: 34372494. Prussin, A. J., Garcia, E. B., & Marr, L. C. (2015). Total Virus and Bacteria.... The nucleic acid of the Bryan strain of Rous sarcoma virus: purification of the virus and isolation ... G. M., & Temin, H. M. (1974). Infectious rous sarcoma virus and reticuloendotheliosis virus DNAs. Journal...M. (1973). RNA-directed DNA polymerase of Rous sarcoma virus: Initiation of synthesis with 70 S viral ...
Showing: 261 - 270 of 795 results