We narrowed to 904 results for: CAN

...so much for how bright you can make a reaction but for how sensitive you can make a reaction. NLuc requires...fluorescent proteins (FPs) can be problematic because excitation light can cause phototoxicity, autofluorescence...tools are fantastic examples of technologies that can accelerate your research. The creation of NanoLuc...by external light sources. These external sources can generate autofluorescence and have limited penetration...protein fusion (OgNLuc). Both fluorescent proteins can be excited by Nluc emission and the lab has dubbed...nm – a clear demonstration of why a BRET reporter can improve deep tissue imaging. The constructs for GpNLuc.... The technique of coupling FPs with Luciferases can overcome these problems but has been hampered by ...

...lab alumni as you can or schedule video/calls if they are not local. Alumni meetings can be the most informative...lab such a big deal? It’s actually something you CAN choose and it will make a huge difference for your...right and responsibility to choose a lab where you can thrive and do your best work. This post is focused...choosing a lab, but almost all of these guidelines can apply to any workplace or job. It amazes me when ...questions in the sections below. Mentoring relationships can help identify your priorities and needs, but sometimes...informative way of gathering data. Former lab members can give open, revealing information about the culture...opportunities with the lab head. Former lab members can give you a fuller picture. Consider talking to people...

...nuclease, can be used in conjunction with Cas13 to amplify the signal of a single target. Csm6 can cleave...laboratories. In the case of the COVID-19 pandemic, qPCR can be used to diagnose the presence of SARS-CoV-2 RNA...reporter. The detectable signal from this reporter can be measured and quantified to determine the presence...an RNA guided RNase (Abudayyeh et al 2016). Cas13 can be guided by a single CRISPR RNA (crRNA) to cleave...non-specific ssRNA cleavage. How does SHERLOCK work? Cas13 can be programmed with crRNA to target an ssRNA of interest...recognizes and binds to the programmed sequence, it can promiscuously cleave surrounding ssRNA molecules....speed, ease of use, and portability. This method can be used for an array of applications including clinical...

...not all cell types can be labeled, and that while multiple copies of the transgene can integrate into cells...recombination is required before fluorescence can occur. Cre recombinase can be used alongside common, existing ...label an organ or entire organism. Because each cell can have multiple copies of the transgene that will recombine...express the construct but do not undergo recombination can be detected by immunostaining of the truncated YFP...and fluoresce in the same color as the fused XFP can be used to amplify the signal. Imaging endogenous... extremely versatile technology as recombination can be induced in developing fish via Cre protein microinjection...mouse (Snippert et al., 2010), a reporter mouse that can be used to track any cell type when crossed with ...

...protein cannot bind. After an inducer binds to the activator protein, the activator protein can bind to...this post, we’ll discuss inducible promoters, which can be switched from an OFF to an ON state, and how you...inducible promoters regulated? Inducible promoters can be regulated by positive or negative control. Positive...temperature, and light are all examples of factors that can lead to the induction of a promoter. Below, you’ll...cAMP complexed with cAMP activator protein (CAP) can also stimulate AraC binding to I1 and I2 sites. Supplementing...near-zero expression at regular temperatures but can be induced by heat or cold exposure. Examples include... pBad is likely a better option since expression can be repressed by glucose. Temperature inducible promoters...

...If there’s a plasmid or AAV tool that you can’t find, you can always suggest a plasmid to us, request that...including a team that created a novel form of AI that can learn new tasks and beat humans at the ancient Chinese...I met one researcher studying how the microbiome can affect Alzheimer's - a connection I wouldn’t have... get into the field, there’s likely some way you can use the tools and skills you’ve developed in your...maybe your favorite FRET-based biosensing technique can be applied to an important neuronal signalling pathway...ve got a useful tool, there’s likely someway you can apply it in neuroscience. AAV tools from Addgene ...useful, these vectors require a bit of expertise and can be difficult for individual labs to make efficiently...

... with large inserts can make lentivirus production frustrating - a researcher can complete an entire lentiviral... SpCas9 and Cas9 nickase for genome editing. You can also enhance expression of your gene of interest ...EGFP, BRAF, and MAP3K4. The gRNAs targeting EGFP can be used as positive controls for gene editing or ...repository are CRISPR pooled gRNA libraries that can be used to screen for genes involved in a wide variety...Getting these quality-controlled reagents from Addgene can greatly expedite a researcher's ability to make discoveries...you’ll also receive transfer plasmid DNA that you can use along with lentiviral packaging plasmids to create...standard lentivirus production protocol here and you can find more tips and protocols for working with lentivirus...

...may not be clearly picked up by the sensors. You can adjust this by changing the voltage for the particular...1). Based on where the GFP- population ends, you can draw a gate which selects anything to the right of...expression, instead of its presence or absence, the plots can look quite different. You might have high expression...upregulated or downregulated based on your experiment. You can see from Figure 2 that determining where to place...quantify smeary plots For smeary expressions, you can quantify shifts in signal using the mean fluorescence...peak that shifts to the left. When using MFI, you can still continue gating populations of interest with...expression to the baseline/negative control. You can see how it is easier to visualize these four populations...

...modification systems, yet can still affect whether certain restriction enzymes can effectively cleave DNA...of mutated plasmids which can later be verified by Sanger sequencing. How can I tell if my enzyme will ... of the DNA surrounding your restriction site(s) can help you quickly predict which (if any) of those ...sequence visualization software (we used Snapgene here) can help you assess whether your restriction site will...in Lane 2. Controlling methylation Finally, you can control methylation by altering your choice of bacteria...that will be blocked by Dam or Dcm methylation, you can ensure this site remains unblocked by first cloning...

...undergo nick repair. This method can work well, but poor template digestion can result in a high number of ...Cloning, and SLIC can all be used to assemble multiple fragments, but these methods can be expensive because... causation in molecular biology and genetics. It can be used to characterize the function of certain regions...biomedical research, modeling patient mutations using SDM can help determine if a variant is causal for a given...strategies, only 3-4 phage proteins are needed and these can be provided via a separate plasmid or be inserted...machinery is induced using L-arabinose. These cells can be made and frozen in large batches suitable for ...