...work with fluorescent proteins, synthetic biology, gene cloning and expression, as well as guide pages for...plasmids made in your lab, see www.addgene.org/deposit or email deposit@addgene.org for more information or ...Addgene will be at the American Society for Microbiology Microbe meeting here in Boston next week - be... and say hello! Just in time for this meeting, Addgene is launching a new resource page for the Microbiology...organisms we should include by emailing us at help@addgene.org. If you are looking for plasmids used in E. .... coli and yeast, you’re in luck! Addgene has so many of these resources that we created filters just ...microbial technology, sign up to write about it on the Addgene blog. ...

...reprogramming transgenes and the up-regulation of corresponding endogenous pluripotency genes. Retroviral...disrupt the expression of tumour suppressor genes and/or oncogenes, potentially leading to the presentation... Shao, L., & Wu, W. (2010). Gene-delivery systems for iPS cell generation. Expert opinion on biological...a single polycistronic vector or multiple single-gene vectors can be used to package reprogramming factors... the transposase. The donor plasmid contains the gene(s) of interest flanked by terminal repeat sequences...demethylation and expression of endogenous pluripotency genes (i.e. Oct4) are other measures of reprogramming....pluripotency: history, mechanisms, and applications." Genes & Development 24, no. 20 (2010): 2239-263. PubMed...

...Resources from the Addgene Blog Addgene's a nonprofit? Get an Inside Look at Addgene's NGS Quality Control... In this episode of the Addgene podcast, we sit down with Addgene’s Founders - Melina Fan, Ken Fan, and...interview with Addgene's founders Podcast breakdown Intro Chapter 1 - Who are Addgene's Founders? Chapter...Chapter 2 - The process of founding Addgene, where the idea for Addgene came from and how the idea got off...Chapter 6 - Developing Addgene’s Brand and culture Chapter 7 - Building Addgene as a global organization...current roles at Addgene and beyond - Ginkgo, Tufts Launchpad, and more Chapter 10 - Addgene's Future, the ...efforts at Addgene as the Chief Financial Officer and Chief Operating Officer. Prior to Addgene, Kenneth...

... also modify other properties such as endogenous gene expression, which can lead to changes in normal ...neuronal firing. What is chemogenetics? Chemogenetics relies on genetically introducing engineered receptors... overview of the chemogenetics system. Why use chemogenetics Because chemogenetic receptors are activated...neuroscience Resources on addgene.org Learn more about chemogenetics in our chemogenetics guide Find viral preps...equipped using fast-acting optogenetic tools. The advantages provided by chemogenetics are instead that it is...longer-lasting effects. Building your chemogenetic system To set up a chemogenetic system, you’ll need to choose...most common target for chemogenetic manipulation are neurons where chemogenetic receptors can be used ...

...Addgene is excited to announce the launch of our partnership with the Institute for Protein Innovation... of integrin antibodies, will be available on Addgene’s website this fall. IPI antibodies will come ... antibody sequences, in line with the rest of Addgene’s recombinant antibody collection, as well as access...developed to support use of their antibody tools. Addgene also offers a growing collection of educational...

...collaboration.  In that post, we talked about Addgene’s data processing needs, our criteria for selecting...Recently, Seqera published a case study on how Addgene has used Nextflow to improve and redesign our pipeline...

...utilizes grants to support our own initiatives, Addgene is deeply concerned about these developments. These... We Are Here to Help Despite these challenges, Addgene remains steadfast in our mission to accelerate ...vital during this period of constrained funding. Addgene is uniquely positioned to help research institutions...Universities and research institutions can turn to Addgene as a trusted partner for archiving their biological...scientific progress depends on our collective response. Addgene stands ready to collaborate with partners across...work and what support would be most valuable from Addgene during this time. Your input will directly shape...scientific community. Let us know by emailing help@addgene.org. We look forward to hearing from you!...

...allow people to add a gene to a repository others could request from — Addgene!  Addgene was established as...information across the globe.  “Read the Addgene guide: https://www.addgene.org/guides/crispr/” — SirHamhands, ... which continue to grow today. “I generally refer people to Addgene for the basics of molecular biology...celebrating Addgene's 10 year anniversary.   Expanding our work In March 2015, Addgene reached the...a resource like Addgene to all get the same virus.” — Grad student visiting Addgene’s booth at the Society...Addgene is ending 2024 with big plans for our future. And while we’ve been excited to share them with...thoughts from the community that makes us… us. “The Addgene not-for-profit is an amazing service to biology...

...perhaps are a little limited on time — check out Addgene’s new Quick Tip series on YouTube! In this series...

... page of the Addgene Data Hub.   Antibody data reports can be searched and sorted by gene/target, name... on addgene.org Addgene's Antibody Collection Addgene's AAV Data Hub More resources on the Addgene blog... request for your experiment!  Addgene’s Antibody Data Hub Addgene’s Antibody Data Hub can help with ...open-access Data Hub, researchers interested in the Addgene antibody collection can look at user-deposited ...experimental conditions in which the data was generated, you can scroll down the page to the materials...each data submission we receive is checked by Addgene's curation team for accuracy and completeness. The... citation. If you’re considering requesting an Addgene antibody, the Data Hub can help you decide if an...