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We narrowed to 928 results for: Gene

Showing: 261 - 270 of 928 results

  1. Improved Plasmid Maps Powered by SnapGene

    Type
    Blog Post
    Published
    June 29, 2017, 1:06 p.m.
    ...ampicillin resistance gene, you’ll find the full name of the gene, the name of its gene product, and a description... plasmid features such as antibiotic resistance genes, selectable markers, promoters, origins, tags, and... Overview of SnapGene powered plasmid maps at Addgene  Three factors make our SnapGene powered plasmid...powered by GSL Biotech's SnapGene Server Software. With the backing of SnapGene’s sequence viewer software...the features you need quickly. SnapGene’s Feature Library - SnapGene’s software seamlessly identifies ...scientists) for suggesting these changes, and the SnapGene and Addgene software development teams for working tirelessly...example below. Our old map is on the left while the SnapGene powered map is on the right (click here to see...

  2. K. phaffii: Rising to the Occasion

    Type
    Blog Post
    Published
    April 4, 2023, 1 p.m.
    ...for its powerful promoter of a gene important for methanol usage. This gene promoter, AOX1, was co-opted...experimental model. In fact, approximately 25% of yeast genes have a human counterpart, enabling study of human-relevant...Saccharomyces cerevisiae to human: The important gene co‑expression modules. Biomedical Reports 7.2 (2017...fast (doubling time of ~ 2 hours) Amenable to genetic manipulation The first eukaryote (S. cerevisiae... new at all, but it’s not as well-known in the general research community. Phillips Petroleum Company...release the K. phaffii expression system to the general research community in the 1990s when they abandoned...the strain tractable for peroxisomal studies on biogenesis, degradation, and proliferation. Genome manipulation...

  3. Multiplexed Overexpression of Regulatory Factors (MORF) collection

    Type
    Blog Post
    Published
    March 30, 2023, 1:15 p.m.
    ... for gene expression, turning transcription on and off by binding proximal to their target genes. Since...Since gene expression patterns determine everything from stem cell differentiation fate to tumor suppression...from the library to further investigate specific genes and TF isoforms. Due to the size of the collection...use to generate the relevant disease model. Accurate modeling of a disease depends on both genetic and expression... not yet available, just ask an Addgene scientist at help@addgene.org! References and Resources  References...scientific community. Recently, the Zhang lab generated a TF library (aka the MORF Collection) and an ...isoforms transcribed from these sites. The Zhang lab generated a comprehensive library of each unique TF open...

  4. How Addgene Creates Content

    Type
    Blog Post
    Published
    Nov. 17, 2022, 2:15 p.m.
    ...everything else we do! The goal of Addgene’s educational content Addgene truly believes that sharing speeds...you ever wondered how we choose topics for the Addgene blog? Or why we make certain videos? In this post...You probably won’t be surprised to learn that Addgene’s mission statement extends beyond plasmids, AAVs...topics. What better space to do that than in the Addgene blog?  Fig. 1: One of our favorite content...content collections is our Help Center, help.addgene.org, where you can easily search articles answering ...sure things are clear, accurate, and in line with Addgene style.        Fig. 2: Reference image (...already comes from you and the questions you ask Addgene and your research community! We work to provide...

  5. Delivery Methods for Generating iPSCs

    Type
    Blog Post
    Published
    April 17, 2018, 1:37 p.m.
    ...reprogramming transgenes and the up-regulation of corresponding endogenous pluripotency genes. Retroviral...disrupt the expression of tumour suppressor genes and/or oncogenes, potentially leading to the presentation... Shao, L., & Wu, W. (2010). Gene-delivery systems for iPS cell generation. Expert opinion on biological...a single polycistronic vector or multiple single-gene vectors can be used to package reprogramming factors... the transposase. The donor plasmid contains the gene(s) of interest flanked by terminal repeat sequences...demethylation and expression of endogenous pluripotency genes (i.e. Oct4) are other measures of reprogramming....pluripotency: history, mechanisms, and applications." Genes & Development 24, no. 20 (2010): 2239-263. PubMed...

  6. Podcast: Interview with Addgene's Founders

    Type
    Blog Post
    Published
    April 13, 2018, 12:51 p.m.
    ...Resources from the Addgene Blog Addgene's a nonprofit? Get an Inside Look at Addgene's NGS Quality Control... In this episode of the Addgene podcast, we sit down with Addgene’s Founders - Melina Fan, Ken Fan, and...interview with Addgene's founders Podcast breakdown Intro Chapter 1 - Who are Addgene's Founders? Chapter...Chapter 2 - The process of founding Addgene, where the idea for Addgene came from and how the idea got off...Chapter 6 - Developing Addgene’s Brand and culture Chapter 7 - Building Addgene as a global organization...current roles at Addgene and beyond - Ginkgo, Tufts Launchpad, and more Chapter 10 - Addgene's Future, the ...efforts at Addgene as the Chief Financial Officer and Chief Operating Officer. Prior to Addgene, Kenneth...

  7. Bringing Renewable Energy to Addgene

    Type
    Blog Post
    Published
    Aug. 27, 2019, 1:08 p.m.
    ....  Additional resources on the Addgene blog Subscribe to the Addgene blog Read our sustainability in...requires a significant energy expenditure here at Addgene. These are critical systems that cannot be removed...only sustainability effort we’ve implemented at Addgene. The kitchen and office areas provide multiple ... the lab as well. For a more in-depth look at Addgene’s current lab-oriented environmental efforts check... about conservation in the lab Resources on Addgene.org Sign up for our quarterly newsletter Help other...resources by sharing plasmids Find plasmids at Addgene ...

  8. It’s CRISPR Week at Addgene!

    Type
    Blog Post
    Published
    May 13, 2019, 12:01 p.m.
    ...social media. #AddgeneCRISPRweek on Twitter From May 13 (today!) to May 17, the Addgene Twitter feed will...CRISPR overview and history. Tuesday will focus on generating knockouts using CRISPR and Wednesday will highlight...CRISPR. Hope you will join us this week at #AddgeneCRISPRweek. We updated the CRISPR guide! If you’re not...

  9. 15 Years of Addgene: The Top 15 Plasmids

    Type
    Blog Post
    Published
    Jan. 8, 2019, 1:54 p.m.
    ...HIV gag, pol, rev, and tet genes. It can be used with 2nd or 3rd generation lentiviral vectors and an ...3rd generation lentiviral packaging plasmid from the Trono lab that contains the gag and pol genes. It...packaging plasmid from the Trono lab contains the rev gene. It’s meant to be used with pMDLg/pRRE and the envelope...doxycycline to the grown media resulting in target gene knockdown. Note: this plasmid was called pLKO-Tet-On... 15 plasmids requested from Addgene psPAX2  - psPAX2 is a 2nd generation lentiviral packaging plasmid...their own or through Addgene!) pX330-U6-Chimeric_BB-CBh-hSpCas9 - The S. pyogenes Cas9 (SpCas9) has been...plasmid predates Addgene’s founding. Find pCMV-VSV-G. pRSV-Rev - This 3rd generation lentiviral packaging...

  10. Viral Vectors 101: Chemogenetics

    Type
    Blog Post
    Published
    July 11, 2023, 1:15 p.m.
    ... also modify other properties such as endogenous gene expression, which can lead to changes in normal ...neuronal firing. What is chemogenetics? Chemogenetics relies on genetically introducing engineered receptors... overview of the chemogenetics system. Why use chemogenetics Because chemogenetic receptors are activated...neuroscience Resources on addgene.org Learn more about chemogenetics in our chemogenetics guide Find viral preps...equipped using fast-acting optogenetic tools. The advantages provided by chemogenetics are instead that it is...longer-lasting effects. Building your chemogenetic system To set up a chemogenetic system, you’ll need to choose...most common target for chemogenetic manipulation are neurons where chemogenetic receptors can be used ...
Showing: 261 - 270 of 928 results