... vector-free cells Genome integration Need to sequence cells to verify excision didn’t introduce mutations...integration Multiple transfections required Need to sequence to verify there’s no genomic integration Replicating...gene(s) of interest flanked by terminal repeat sequences, which the PB transposase recognizes. Following...can occur at the transposon insertion site, so sequence-verification is required. Non-integrating...

...editing for cleavage-free genomic knock-in of long sequences🎵On the 2nd day of CRISPR, Addgene gave to me:...

...signal sequence and to the mitochondria via citrate synthase’s mitochondrial targeting sequence. Under...

...diseases. Spontaneous mutations that change protein sequence affect arginine (Arg) amino acids more frequently...facilitated by CRISPR reagents and other specific gene sequences available from Addgene. Dr. McMurray hopes that...metabolic diseases with severe and life-threatening consequences. They result from defects in different steps...

...CRISPR and RNAi have helped researchers alter DNA sequence and RNA expression - but what if you just want...localization, binding to the appropriate promoter sequence and activation of gene expression. In addition...

...gene may have errors introduced to the nucleotide sequence during the DNA repair process. The addition of...recombinase catalyzes the recombination of DNA sequences located between two loxP sites. The loxP sites...

...approaches that involve modifying the antibody sequence or that rely on strong protein-protein interactions...Lysine residues are found throughout antibody sequences, up to 80 per antibody (Mueller, et al., 1988)...

...plasmid encoding the insert of interest as well as sequences necessary to encapsulate that insert into a viral...plasmid and the packaging plasmid caused the Psi sequence to be placed on the packaging plasmid forming ...composed of the wild-type 5’ and 3’ LTRs, the ψ sequence, a part of the env gene containing the rev response...

...domain can be engineered to target specific DNA sequences while FokI then induces a double strand break.... strategy of using a protein to recognize DNA sequences is not ideal. For the early generation gene editors...protein-based DNA-binding domain with a single-guide RNA sequence.  The Cas proteins, just like its predecessor ...

...[2]. Delivering dsRNA matching a specific mRNA sequence triggers a cascade of events that results in the...genes not in this collection, they may clone DNA sequences for creating riboprobes and dsRNAs into PR-T4P...