Skip to main content
Addgene
Showing: 521 - 540 of 779 results
  1. Antibodies 101: Chimeric Antibodies

    Type
    Blog Post
    Published
    April 25, 2023, 1:15 p.m.
    ...You are a scientist looking to determine how Protein A and Protein B interact. You read extensive research...Robinson, M. K., Graham, E. T., Sabo, J. P., Owens, R., King, D. J., Norris, S. H., & Scher, D. S. (1994). Isotype...
  2. What the HEK?

    Type
    Blog Post
    Published
    April 18, 2023, 1:15 p.m.
    ... four. No Y chromosome is present in this line, making it genetically female. HEKs are generally cultured... line for you. Are HEKs for you?   If you are looking for a human cell line model, HEKs might be a great...
  3. Summer SciComm: Science Storytelling

    Type
    Blog Post
    Published
    Sept. 16, 2022, 1:15 p.m.
    ...by outright stating the big-picture problem, by asking your audience a simple question, like “Have you...ending of the story.  At this point you may be thinking  “I don’t want to bury my results all the way ...
  4. Celebrate National Book Month with Addgene

    Type
    Blog Post
    Published
    Oct. 3, 2019, 12:47 p.m.
    ...Incredibly imaginative, funny, bittersweet, and shocking at times."     - Jason Snair, Product Designer...on today! Addgene Book Club (ABC) founder, Eric Perkins, is Addgene’s Director of Science Product Management...
  5. New and Upcoming Viral Vectors - June 2019

    Type
    Blog Post
    Published
    June 18, 2019, 1:15 p.m.
    ...contrast” variant provides improved detection for tracking large populations of neurons in wide-field imaging...fusion fluorescent proteins can affect DREADD trafficking and localization. The Sternson lab recently deposited...
  6. Plasmids 101: Gram Negative and Positive Bacteria

    Type
    Blog Post
    Published
    Aug. 2, 2022, 1:15 p.m.
    ...and how it can be used in a lab? Here we’ll be talking about the ins and outs of this classification system...negative (red/pink) staining. Image credit: Mark Perkins https://www.flickr.com/photos/occbio/7092385559...
  7. Using CRISPR/Cas9 to Edit Disease Out of the Genome

    Type
    Blog Post
    Published
    June 23, 2022, 1:15 p.m.
    ...effectiveness of CRISPR/Cas9, scientists are still looking for ways to diminish the possibility of those off-target...s Disease.” Journal of Alzheimer's disease & Parkinsonism. https://doi.org/10.4172/2161-0460.1000439 6...
  8. Early Career Researcher Toolbox: Free Tools for Science Writing

    Type
    Blog Post
    Published
    April 28, 2021, 1:15 p.m.
    .... There’s also tips for setting writing goals, tracking progress, and starting a writing group. Loop Habit...literally a tomato! Image from Gerlos.   Tools for checking grammar and jargon Hemingway Editor  This is a...
  9. Viral Vectors 101: Inverted Terminal Repeats

    Type
    Blog Post
    Published
    Oct. 19, 2023, 1:15 p.m.
    ...proteins and mutations in the trs site reduce Rep nicking. Since Rep proteins are important for viral genome...PMID: 28967288. Shitik, E. M., Shalik, I. K., & Yudkin, D. V. (2023). AAV- based vector improvements unrelated...
  10. Trading Spaces - Visiting Scientist Edition

    Type
    Blog Post
    Published
    Aug. 22, 2023, 1 p.m.
    ...you have one) to communicate your plan as well. Making logistical and funding arrangements Visiting scientist...really good time! If it sounds right for you, start thinking outside of your current research box, send out...
  11. The MTA at Addgene

    Type
    Blog Post
    Published
    July 23, 2024, 2:17 p.m.
    ...centralized tracking when biological reagents are shared — both at what time and with whom. Tracking these ...
  12. Predicting Adverse Reactions to Monoclonal Antibody Drugs

    Type
    Blog Post
    Published
    Feb. 13, 2024, 2:15 p.m.
    ...differentiated into dentritic cells or macrophages through cytokine induction (Groell et.al, 2018). Thus, microscopic...mimic the human lymphatic system or even the human skin and subcutaneous models (Groell et.al, 2018). Treating...
  13. Optimizing Donor DNA for Enhanced CRISPR Genome Editing

    Type
    Blog Post
    Published
    March 24, 2016, 2:30 p.m.
    ...diseases in which sequence replacement holds some kind of fitness advantage but error-prone repair of the... the gene would be disastrous (i.e. if breaking the gene is worse than leaving the mutation alone). CRISPR...
  14. Fluorescent Proteins 101: Luciferase

    Type
    Blog Post
    Published
    June 24, 2014, 3:59 p.m.
    ...Photons Chemical reaction High-energy photons Kinetics of Photon Generation Slower Faster Cofactors...been identified in various species with differing kinetics, substrate requirements and photon emission wavelengths...
  15. Screening for Successful Genome Editing with Digital PCR

    Type
    Blog Post
    Published
    March 30, 2017, 2:30 p.m.
    ...performed to amplify the target using PCR. Instead of tracking the reaction progress as in real time PCR, each...introduced how digital PCR works, let’s explain what kind of assays can be used to sniff out your desired ...
  16. Addgene’s Viral Service - Why Virus? Why Now?

    Type
    Blog Post
    Published
    March 23, 2017, 2:30 p.m.
    ... and careful Addgenies, but also a lot of good tracking. Our Software and IT Teams made major upgrades...plasmids in the repository. The Outreach Team has been making sure scientists know about the new initiative and...
Showing: 521 - 540 of 779 results