We narrowed to 901 results for: IND

...your selection method kills the transgene free individual. For example, with CRISPR-Cas9 gene editing,...experiments can only be collected from heterozygous individuals. Only 1/4 of the descendants of the heterozygous...

...toxic in high amounts? It is always good to keep in mind that plasmids with low to medium copy numbers can... considerations are especially useful to keep in mind if you are planning to purify your plasmid DNA: ...

...stormy times. It feels like we’re being buffeted by wind and waves and sometimes wonder if we can take another...SARS-CoV-2 would be fully open and available to industry requestors as well. This is a time when we need...

...inherently unstable due to their secondary structure, palindromic nature, and high GC content. This instability...expression in retinal cells Resources on Addgene.org Find AAVs for your experiment Browse our AAV protocols...

...piece of equipment that is custom-built, hard to find, or inaccessible to you for another reason. Visiting...visiting position. What now? First, you’ll need to find someone who is willing to host you! If there is ...

...recombinant proteins by the pharmaceutical and biotech industries as a protein expression workhorse. This yeast...into methanol and peroxisomal autophagy can be induced by removing methanol from the media. This feature...

...little bit. You can just go to sessions that you find interesting and not go to the rest.” Having an on-site...blog Read all of our blog posts about conferences Find 25 tips for working from home ...

...journals and publishers. As journal editors, we often find ourselves having to ask authors for additional information...Press. Additional resources on the Addgene blog Find more ways to make your research more reproducible...

...get to annotate things as they come up in their minds. The final screen can also be saved and exported...PhDs: Swimming upstream a career from academia to industry”. To date, aside from doing science, he has assisted...

...free to play around with different strategies and find out what works for your organization. Ask for feedback...Advice from Addgene Executive Director Joanne Kamens FInd Tips on Picking a Research Project Read Other Inside...

...system or by filtering by fly as species of gene. Find Gal4 and UAS plasmids at Addgene Choosing a vector...experimentation. Drosophila are amenable to many different kinds of experimental assays depending on your interests...

...Resources at Addgene.org Find Virus Protocols Visit Our Viral Vector Pages Find Virus for Your Research...

... the only informal synthetic biology club of its kind, running from start to finish during the academic... Addgene.org Guide to SynBio Assembly Standards Find Plasmids for Creating and Controlling Gene Networks...

...Another similarity to Cas12a is that Cas14 also indiscriminately cleaves non-complementary ssDNA when engaged... the genome. Cas13, similar to Cas14, also indiscriminately cleaves ssRNA when engaged at its RNA target...

...method it is not possible to follow turnover of individual proteins in situ.  For the visualization of specific...Söderberg, Ola, et al. "Direct observation of individual endogenous protein complexes in situ by proximity...

...request from us but also by high school students and industry scientists as a universal resource for information...about Addgene Resources on the Addgene website Find plasmids for your research  Learn about our viral...

...the repressor because TALEs can be programmed to bind tightly to arbitrary DNA sequences and have been...100 fold repression in E. coli. In addition, TALEs bind non-cooperatively to the promoter region, which ...

...decontaminating liquid waste, remember that the percentage indicates the proportion of the decontaminate in the waste...things they work with, and that can understandably induce some nerves. Following safety procedures and practicing...

...research (e.g., AAV) are useful for labeling the individual neurons they infect. However, they generally ...Expression AAV for Rabies Glycoprotein (G) expression  Find Retrograde AAV ...

... question falls. Here are some things to keep in mind after you hit “send” or dial that last digit: An...why you haven’t received a response, just keep in mind that our customer support scientists wear many hats...

...and compliance for the FLSA changes, including finding money in already tight lab budgets and even simply... Learn to Advocate with the Perfect Science Talk Find a Good Mentor ...

...challenging to compete in today’s tough academic or industry job market for biology PhD’s. I was facing the...plants engineered to detect explosives and rapidly indicate their presence. They are genetically modified ...

...wavelengths (See this handy emission spectra chart to find your antibody's spectra). Your cytometer will therefore...diagonal population, this is a sign of spillover and indicates that it is necessary to apply a compensation value...

... Sybr Green is a fluorescent dye that works by binding to all double-stranded DNA, while Taqman uses an...variance in your variant options, you’re likely to find an application that matches your needs. Happy quantifying...

...allows CAPTURE 2.0 to be used with primary cells. Find plasmids for CAPTURE 2.0! When compared to the original... CREs Here Additional Resources on Addgene.org Find Other Cas9 Variants for Purifying Genomic Regions...

...the University of Miami Miller School of Medicine. Find plasmids from the Lee Lab here. Pantelis Tsoulfas...the University of Miami Miller School of Medicine. Find plasmids from the Tsoulfas lab here.     References...

... to build a gene drive in a model organism would find our work and consequently use these precautions ...geographic area where escaped organisms won't be able to find mates2. For example, ongoing experiments attempting...

...-expression by using a plasmid with multiple, individual expression cassettes, having the genes expressed... the ability to spark translation initiation independent of the 5’ cap.  IRES elements are very useful...

...science with BioBricks The BioBrick Foundation's Linda Kahl explains the Addgene collaboration this way...analyzing human language and observed that we humans find it useful to describe and interact with the world...

...2015). Total Virus and Bacteria Concentrations in Indoor and Outdoor Air. Environmental Science & Technology...RT and IN incorporated into HIV-1 particles independently of the Gag/Pol precursor protein. The EMBO Journal...