We narrowed to 610 results for: ACE;
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TypeBlog PostPublishedJan. 31, 2019, 1:32 p.m....2009). Then, scientists began focusing on the extracellular matrix and fibroblasts. With each cell mechanism...
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CRISPRainbow and Genome Visualization
TypeBlog PostPublishedFeb. 28, 2017, 3:30 p.m....ortholog, and the target sequence must be located adjacent to that PAM. To scale up fluorescent CRISPR labeling... -
Uncertainty about Labor Law Brings More Uncertainty to Postdoc Wages
TypeBlog PostPublishedJan. 11, 2017, 3:30 p.m....getting a raise. Meanwhile, in less densely populated places like the University of Kansas and the University... -
Plasmids 101: CcdB - The Toxic Key to Efficient Cloning
TypeBlog PostPublishedDec. 8, 2016, 3:30 p.m....contains ccdB. A successful insertion will completely replace ccdB with the investigator’s insert of interest... -
Transferable Skills Guide: Public Speaking
TypeBlog PostPublishedApril 20, 2023, 4 p.m....incredibly calm: “Excuse me, I seem to have lost my place. I need a minute.” I then pulled out my notebook... -
Addgene: The First Twenty Years
TypeBlog PostPublishedDec. 19, 2024, 2:15 p.m....hottest phone on the market, and two new websites, Facebook and Gmail, were launched. And Melina Fan, a cell... -
The time and cost required to make a plasmid
TypeBlog PostPublishedMarch 23, 2023, 1:15 p.m....resources, and, on the rare occasion things go wrong, replace them for free. Plasmids ship out 2-3 days after... -
All in a Twist: dsRNA
TypeBlog PostPublishedFeb. 2, 2023, 2:15 p.m.... RNA Mammalian cells have multiple receptors in place to sense dsRNA and respond accordingly. While not... -
Gendered Innovations: Why Does Sex of the Cell Matter?
TypeBlog PostPublishedJuly 21, 2016, 2:30 p.m....effects on the sexes come from, one clue or potential place to start research is by considering sex disparities... -
Addgene's Tips for Plasmid Quality Control
TypeBlog PostPublishedJan. 14, 2016, 3:30 p.m....when designing a sequencing primer, you need some space between your primer binding site and the first feature... -
Plasmids 101: Golden Gate Cloning
TypeBlog PostPublishedAug. 27, 2015, 2:30 p.m.... The destination vector and entry vector(s) are placed in a single tube containing the Type IIS enzyme... -
Writing Scientific Manuscripts: Literature Searching, Reading, & Organizing
TypeBlog PostPublishedMay 5, 2015, 3:54 p.m.... – F1000 just announced the launch of their F1000Workspace. Give your favorite searching, reading and... -
Career Coaching for Scientists: Why and Where Do I Find One?
TypeBlog PostPublishedJan. 29, 2015, 1:25 p.m....articles. Look for them on Twitter, LinkedIn and Facebook to see their online personas. Some have online... -
5 Steps to Writing Grant Proposals for Grad Students
TypeBlog PostPublishedFeb. 11, 2014, 12:13 p.m....research plan Most grant applications restrict the space available to describe the proposed work. Thus, every... -
CRISPR Activators: A Comparison Between dCas9-VP64, SAM, SunTag, VPR, and More!
TypeBlog PostPublishedOct. 6, 2020, 1:15 p.m....method, dCas9 is fused to CBP, an histone acetyltransferase domain capable of rearranging chromatin structure... -
CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems
TypeBlog PostPublishedJuly 23, 2020, 1:20 p.m.... viruses. For example, an Acr gene was used to replace genes in the difficult-to-engineer Sulfolobus islandicus... -
Corralling and Cornering the Goldilocks Antibody
TypeBlog PostPublishedAug. 9, 2022, 1:15 p.m.... contained all the goldilocks antibodies in one place... well, lucky for you one is in the making by Dr... -
Split Fluorescent Proteins for Studying Protein-Protein Interactions
TypeBlog PostPublishedMay 3, 2022, 1:15 p.m....fragment An enhanced recombinant amino-terminal acetylation system and novel in vivo high-throughput screen... -
Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation
TypeBlog PostPublishedDec. 14, 2021, 2:15 p.m.... godsends. Tethering dCas9 to the histone acetyltransferase p300 or the DNA methyltransferase DNMT3A allows... -
Adenoviral Vector Production and Troubleshooting
TypeBlog PostPublishedJune 14, 2018, 11:44 a.m....chloride (CsCl) density gradients combined with ultracentrifugation to separate rAdV from other cellular debris...