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Showing: 521 - 540 of 801 results

  1. Management for Scientists: Giving Feedback

    Type
    Blog Post
    Published
    Feb. 6, 2015, 2:46 p.m.
    ...respond to the employee’s level of discomfort appropriately to get the message across. Listen to our podcast...to you. What are We Learning: What would have happened if _________? What would have been a better outcome...

  3. Plasmids 101: Antibiotic Resistance Genes

    Type
    Blog Post
    Published
    Jan. 30, 2014, 3:29 p.m.
    ...and can be used in place of Ampicillin in most applications. Antibiotics vary in their sensitivitly to heat...hotter than about 55oC and store plates/stocks wrapped in foil if a light-sensitive antibiotic like Tetracycline...

  4. Single-cell tracking of lineage and identity with CellTag

    Type
    Blog Post
    Published
    May 9, 2019, 1:15 p.m.
    ...not silenced.    Find CellTag libraries here!  Applications of CellTag The Morris Lab developed CellTag ...References Biddy, Brent A., et al. "Single-cell mapping of lineage and identity in direct reprogramming...

  5. New and Upcoming Viral Vectors - Spring 2019

    Type
    Blog Post
    Published
    March 26, 2019, 1:40 p.m.
    ...kinetics) that makes them suitable for different applications. We recently released a new variant of jGCaMP7... been developed and are suited for different applications. The iGABASnFR GABA sensor (pAAV.hSyn-FLEX.iGABASnFR...

  6. Antibodies 101: Epitope Availability

    Type
    Blog Post
    Published
    Jan. 11, 2022, 2 p.m.
    ...categories: ones induced by the researcher and ones that happen naturally.  Sample Denaturing  First, let’s think...SDS-page gel. As the protein unfolds, new epitopes may appear, while others may get hidden. Monoclonal antibodies...

  8. Viral Vectors 101: What is a Virus (and a Viral Vector)?

    Type
    Blog Post
    Published
    Aug. 18, 2023, 4:15 p.m.
    ...into two cells. Viral vectors Viruses are quite appealing as molecular biology tools… if one isn’t too bothered...on the Addgene blog Viral Vectors 101: Viral Applications Viral Vectors 101: Viruses as Biological Tools...

  9. What Do I Do Now? Academic v. Non-Academic Career Decisions

    Type
    Blog Post
    Published
    March 9, 2017, 3:40 p.m.
    ...can do better, no grant writing Job changes can happen regularly (every 4-6 years is common), but this...issues can create interpersonal issues (one bad apple can change entire culture) Specific requirements...

  11. CRISPR Challenges: Standardization and Homology Directed Repair

    Type
    Blog Post
    Published
    Feb. 14, 2018, 2:36 p.m.
    ...the gRNA design process and rules for various applications is necessary going forward. The standardization...Alt-NHEJ), has been harnessed for CRISPR knock-in applications. CRISPR 101: Targeting RNA with Cas13a (C2c2...

  12. Identifying Sequence Elements with SnapGene's Feature Database

    Type
    Blog Post
    Published
    Dec. 21, 2017, 2:06 p.m.
    ...response to customer feedback. An example of this approach is SnapGene’s algorithm for detecting common features.... We are working on a way for SnapGene users to apply features from a reference strain to newly sequenced...

  13. Plasmids 101: TOPO Cloning

    Type
    Blog Post
    Published
    Oct. 27, 2016, 2:30 p.m.
    ...“A” overhang insert, the magic of TOPO cloning happens. Basic procedure Let’s break down the steps needed...1658796. PubMed Central PMCID: PMC52876. 2. Novel approach to molecular cloning and polynucleotide synthesis...

  14. Summer SciComm: Science Storytelling

    Type
    Blog Post
    Published
    Sept. 16, 2022, 1:15 p.m.
    ... work.  And while these pieces aren't always appropriate for your formal scientific communications, they...still get invested in the journey? The same thing applies for science storytelling. You can introduce your...

  18. Another Pathway into Cells: iTOP

    Type
    Blog Post
    Published
    June 23, 2015, 8:37 p.m.
    ...protein-only transduction method and its potential applications to CRISPR/Cas9 genome editing.   Protein-only...Diego D'Astolfo. CRISPR/Cas9: a potential application of iTOP Having tested iTOP using Cre-lox recombination...

  19. Extracurricular Activities for a Strong Science Career Path

    Type
    Blog Post
    Published
    Jan. 21, 2014, 3:08 p.m.
    ...multidisciplinary, collaborative, and translational approaches for scientific tool and technique development...leadership, management, and communication. A great approach to fully understand and acquire competitive skill...

  20. Reagent Repositories Are Speeding up Science During the Pandemic

    Type
    Blog Post
    Published
    Aug. 4, 2020, 1:15 p.m.
    ... Another example of reagents that have broad applications but have proven useful to the study of SARS-...et al., 2006). Now that a similar phenomena is appearing with regards to SARS-CoV-2, this mouse strain ...
Showing: 521 - 540 of 801 results